Monoclonal Antibodies to Identify Tomato Mosaic Tobamovirus (Tomv)

Duarte, Keila Maria Roncato; Gomes, Luiz Humberto; Gesztesi, J.-L.; Lopes, José Daniel; Tavares, Fernanda Silveira

doi:10.1590/s1517-83822001000300016

Cited by 6 publications

(2 citation statements)

References 12 publications

(11 reference statements)

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“…Monoclonal antibodies for ToMV detection are being developed with the aim to eliminate cross-reactivity with other tobamoviruses invading tomatoes [39]. However, even these may not always be able to completely eliminate cross-reactivity with other Tobamoviruses, e.g., with Tobacco Mosaic Virus (TMV).…”

Section: Discussionmentioning

confidence: 99%

Evaluation of New Polyclonal Antibody Developed for Serological Diagnostics of Tomato Mosaic Virus

Mrkvová

Hančinský

Grešíková

et al. 2022

Viruses

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Plant viruses threaten agricultural production by reducing the yield, quality, and economical benefits. Tomato mosaic virus (ToMV) from the genus Tobamovirus causes serious losses in the quantity and quality of tomato production. The management of plant protection is very difficult, mainly due to the vector-less transmission of ToMV. Resistant breeding generally has low effectiveness. The most practical approach is the use of a rapid diagnostic assay of the virus’ presence before the symptoms occur in plants, followed by the eradication of virus-infected plants. Such approaches also include serological detection methods (ELISA and Western immunoblotting), where antibodies need to be developed for an immunochemical reaction. The development and characterization of polyclonal antibodies for the detection of ToMV with appropriate parameters (sensitivity, specificity, and cross-reactivity) were the subjects of this study. A new polyclonal antibody, AB-1, was developed in immunized rabbits using the modified oligopeptides with antigenic potential (sequences are revealed) derived from the coat protein of ToMV SL-1. the developed polyclonal antibody. AB-1, showed higher sensitivity when compared with commercially available analogs. It also detected ToMV in infected pepper and eggplant plants, and detected another two tobamoviruses (TMV and PMMoV) and ToMV in soil rhizosphere samples and root residues, even two years after the cultivation of the infected tomato plant.

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Section: Discussionmentioning

confidence: 99%

Evaluation of New Polyclonal Antibody Developed for Serological Diagnostics of Tomato Mosaic Virus

Mrkvová

Hančinský

Grešíková

et al. 2022

Viruses

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show abstract

“…Since the protocol was optimised in the past, the improvement of the serological detection method with monoclonal antibodies was not considered. Duarte, Gomes, Gesztesi, Lopes, and Tavares (2001) who obtained monoclonal antibodies against ToMV reported the low efficiency of such antibody production where only four possible monoclonal antibodies could be produced in low concentrations. Our developed RT real-time PCR was shown to be five orders of magnitude more sensitive than the serological method.…”

Section: Discussionmentioning

confidence: 99%

Detection and quantification of Tomato mosaic virus in irrigation waters

et al. 2007

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A quantitative RT real-time PCR method was developed for the detection and quantification of Tomato mosaic virus (ToMV) in irrigation waters. These have rarely been monitored for the presence of plant pathogenic viruses, mostly due to the lack of efficient and sensitive detection methods. The newly developed method presented here offers a novel approach in monitoring the health status of environmental waters. ToMV was reliably detected at as low as 12 viral particles per real-time PCR reaction, which corresponds to the initial concentration of approximately 4.2 · 10 -10 mg (6,300 viral particles) of ToMV per ml of sample. The sensitivity of the method was further improved by including the Convective Interaction Media Ò (CIM) monolithic chromatographic columns for quick and efficient concentration of original water samples. Seven out of nine water sources from different locations in Slovenia tested positive for ToMV, after concentrating the sample. Four samples tested ToMV-positive without the concentrating procedure. The presence and integrity of infective ToMV particles in the original sample, as well as in the chromatographic fraction, was confirmed using different methods from test plants, DAS ELISA to electron microscopy and real-time PCR. In this study, we propose a unique and simple diagnostic scheme for rapid, efficient, and sensitive monitoring of irrigation waters that could also be adopted for other plant, human or animal viruses.

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Responses of Helicoverpa armigera to Tomato Plants Previously Infected by ToMV or Damaged by H. armigera

Shen

Chen

et al. 2008

J Chem Ecol

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We report the comparative inducing effects of a phytopathogen and a herbivorous arthropod on the performance of an herbivore. Tomato, Lycopersicon esculentum Mill., was used as the test plant, and tomato mosaic virus (ToMV) and corn earworm, Helicoverpa armigera Hübner, were used as the phytopathogen and herbivore, respectively. There were decreases in the efficiency of conversion of ingested food and efficiency of conversion of digested food when H. armigera was reared on tomato plants that had been previously inoculated with ToMV. However, virus inoculation did not affect feeding or oviposition preferences by H. armigera. In contrast, approximate digestibility, total consumption, relative growth rate, and relative consumption rate were lower for fourth-instar H. armigera that fed on plants previously damaged by the same herbivore. Feeding and oviposition were both deterred for H. armigera that fed on previously damaged plants. The duration of development of H. armigera was also prolonged under this treatment. Infection by ToMV and feeding damage by H. armigera increased the host plant's peroxidase and polyphenol oxidase activity, respectively, suggesting that the performance of H. armigera may be affected by the induced phytochemistry of the host plant. Overall, this study indicated that, in general, insect damage has a stronger effect than ToMV infection on plant chemistry and, subsequently, on the performance of H. armigera.

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Monoclonal Antibodies to Identify Tomato Mosaic Tobamovirus (Tomv)

Cited by 6 publications

References 12 publications

Evaluation of New Polyclonal Antibody Developed for Serological Diagnostics of Tomato Mosaic Virus

Evaluation of New Polyclonal Antibody Developed for Serological Diagnostics of Tomato Mosaic Virus

Detection and quantification of Tomato mosaic virus in irrigation waters

Responses of Helicoverpa armigera to Tomato Plants Previously Infected by ToMV or Damaged by H. armigera

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