2001
DOI: 10.1590/s1517-83822001000200015
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Abstract: The genomic DNA of thirty strains of Aujeszky's disease virus (ADV) isolated in the South and Southeast regions of Brazil from 1982 to 1996 were characterized by restriction endonuclease analysis with BamHI. Twenty seven strains were isolated from pigs, 1 from cattle, 1 from cat and 1 from dog. Using a systematization previously described, the 30 ADV strains could be classified as genomic types I (n = 2) and II (n = 28). Genomic type III was not observed. In this first study of genomic type characterization of… Show more

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Cited by 13 publications
(13 citation statements)
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“…Moreover, the virus isolated in 1989 showed a genomic type never found before in that State (genomic arrangement type I), once PRV isolates from the recent outbreak (2003) showed a genomic arrangement type II. In Brazil, PRV isolates showing a genomic arrangement type I had only been identified in pigs, from São Paulo State, in 1986 and 1990 (14). In our work, two other samples isolated in Toledo, Paraná State in 1984 were characterized as genomic arrangement type Ii.…”
Section: Resultsmentioning
confidence: 47%
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“…Moreover, the virus isolated in 1989 showed a genomic type never found before in that State (genomic arrangement type I), once PRV isolates from the recent outbreak (2003) showed a genomic arrangement type II. In Brazil, PRV isolates showing a genomic arrangement type I had only been identified in pigs, from São Paulo State, in 1986 and 1990 (14). In our work, two other samples isolated in Toledo, Paraná State in 1984 were characterized as genomic arrangement type Ii.…”
Section: Resultsmentioning
confidence: 47%
“…In Brazil, previous work has identified genomic arrangement type II as the most predominant in 28 out of 30 PRV isolates analysed (14). In that work, all 19 PRV isolates from Santa Catarina State showed a genomic arrangement type II.…”
Section: Resultsmentioning
confidence: 84%
“…A nested-PCR foi 100 vezes mais sensível do que uma PCR convencional descrita na literatura (BASCUÑANA et al (1997). A caracterização genotípica do PrV por análise de restrição enzimática foi utilizada por vários autores em estudos epidemiológicos, sendo o método baseado na restrição do genoma completo do PrV com BamHI, com variações no método de purificação do DNA viral, o mais utilizado (CHRISTENSEN, 1987;CHRISTENSEN, 1988;NISHIMORI et al, 1987, YAMADA et al, 1992CHRISTENSEN, 1995;PIATTI et al, 2001;SILVA et al, 2005;SCHAEFER et al, 2006). Isolados de campo foram identificados por esse mesmo método como derivados de amostras vacinais na Polônia e Hungria (CHRISTENSEN et al, 1992).…”
Section: Discussionunclassified
“…Vinte amostras do PrV isoladas nas Regiões Sul e Sudeste do Brasil previamente genotipadas (PIATTI et al, 2001;SILVA et al, 2005) e a estirpe padrão Shope foram multiplicadas em células PK-15 (Tabela 1). A estirpe padrão Shope, genótipo I, foi utilizada como controle positivo em todos os testes descritos neste trabalho.…”
Section: Amostrasunclassified
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