Evidenciadores de biofilme em prótese total: avaliação clínica e antimicrobiana

Silva‐Lovato, Cláudia Helena; Paranhos, Helena de Freitas Oliveira; Ito, Isabel Yoko

doi:10.1590/s1517-74912002000300015

Cited by 29 publications

(21 citation statements)

References 16 publications

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“…The disclosing agent method is the most widely applied to complete dentures. Its association to quantitative methods 3,6,9,15,19,23,27,29 is less common. However, few studies discuss the application and reliability of these methods 4,8,18,23,24 .…”

Section: Discussionmentioning

confidence: 99%

“…Both methods presented sensitivity for the detection of biofilm levels in complete dentures 8 . The paper-weighing method was previously applied to natural teeth 12 and complete dentures 22,29 and its reproducibility was tested 23 . The use of the computerized method is relatively new for complete dentures.…”

Section: Imagetoolmentioning

confidence: 99%

“…The computerized of good coloration and easy removal from the denture. It is important to note that when biofilm is to be measured on denture bases made of clear acrylic resin, a colored background to contrast with the disclosing agent should be used 11,29 . In the present study, clear-base dentures were not selected, in order to eliminate this variable.…”

Section: Imagetoolmentioning

confidence: 99%

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Comparative study of methods for the quantification of biofilm on complete dentures

Paranhos

Silva‐Lovato

2004

Braz. oral res.

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This study compared the application and reliability of 4 methods for biofilm quantification (computerized, paper-weighing, point-counting, and planimetric) in complete dentures, verifying the correlation between them. The internal surfaces of 62 complete dentures were stained (5% erythrosine) and photographed. The slides were projected on paper, and the areas (total and biofilm-covered) were outlined with a pencil. These areas were measured with an equidistant point grid (point-counting method), a digital planimeter (planimetric method), and for the paper weighing method they were cut and weighed with a precision scale. For the computerized method, ImageTool software was used. In order to perform a validation test of the methods, all of them were applied to slide projections of geometric figures with known dimensions. The correlation tests showed high correlation values (r = 0.82 to 0.99) among the methods. The validation test (ANOVA) showed no statistically significant differences among the values obtained from the measurement of figures using all four quantitative methods and the real dimensions of these geometric figures. Quantitative methods were efficient and reliable for measuring quantity of biofilm in complete dentures, and may be useful in experimental studies on the efficacy of hygiene products. The computerized method was fast and easy to perform. DESCRIPTORS: Denture, complete; Biofilms. RESUMO:Este estudo comparou a aplicação e a confiabilidade de quatro métodos de quantificação de biofilme (computadorizado, pesagem de papel, contagem de pontos e planimétrico) em próteses totais, verificando a correlação entre eles. As superfícies internas de 62 próteses totais foram coradas (eritrosina a 5%) e fotografadas. Os diapositivos foram projetados em papel, e as áreas (total e com biofilme corado) foram contornadas com grafite. Estas áreas foram medidas com uma grade de pontos eqüidistantes (método de contagem de pontos), um planí-metro digital (método planimétrico) e, para o método de pesagem de papel, foram recortadas e pesadas em balança de precisão. No método computadorizado, foram medidas com um software (ImageTool). Com o objetivo de realizar um teste de validação dos quatro métodos, estes foram aplicados também em diapositivos de figuras geométricas de dimensões conhecidas. Os testes de correlação mostraram altos valores de correlação (r = 0,82 a 0,99) entre os métodos. O teste de validação (ANOVA) não mostrou diferença estatisticamente significante entre as medidas reais dos desenhos geométricos e aquelas obtidas pelos quatro métodos quantitativos. Os métodos quantitativos mostraram-se eficazes e confiáveis na mensuração dos níveis de biofilme em próteses totais, podendo ser úteis em estudos experimentais da eficiência de produtos de higiene. O método computadorizado mostrou-se rápido e de fácil aplicação.

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Section: Discussionmentioning

confidence: 99%

Section: Imagetoolmentioning

confidence: 99%

Section: Imagetoolmentioning

confidence: 99%

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Comparative study of methods for the quantification of biofilm on complete dentures

Paranhos

Silva‐Lovato

2004

Braz. oral res.

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“…In this study, the staining of bacterial plaque was made by using an aqueous solution of 2% eosin. We opted for the eosin in this study because it does not present antimicrobial activity and is easily removed from the tooth surface (Silva et al 2002). These features make eosin an advantageous option for assessing specific products with antiplaque action.…”

Section: Discussionmentioning

confidence: 99%

Efficacy of a Kalanchoe gastonis-bonnieri extract to control bacterial biofilms and dental calculus in dogs

et al. 2017

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RESUMO.-[Eficácia do extrato de Kalanchoe gastonis--bonnieri no controle do biofilme bacteriano e cálculo dentário em cães.] Um extrato aquoso de folhas da espé-cie medicinal Kalanchoe gastonis-bonnieri (aqui denominado como KGB) foi efetivo como um agente antimicrobiano contra as bactérias da cavidade oral de cães em testes in vitro. Neste estudo, investigou-se o efeito da administração oral tópica de KGB sobre o desenvolvimento do biofilme dental em cães da raça Beagle. Os experimentos foram realizados com um grupo experimental (0,2% de extrato de KGB), um grupo controle negativo (0,9% de solução salina) e um grupo controle positivo (0,12% de gluconato de clorexidina). Cada tratamento foi aplicado no interior da ca- An aqueous leaf extract of the medicinal species Kalanchoe gastonis-bonnieri (here denominated KGB) has been found to be effective as an antimicrobial agent against canine oral cavity bacteria in in vitro assays. In this study, we investigated the effect of topic oral administration of KGB on the development of dental biofilm in Beagle dogs. The experiments were performed with an experimental group (0.2% of KGB extract), a negative control group (0.9% of saline solution) and a positive control group (0.12% chlorhexidine). Each treatment was sprayed into the oral cavity daily for 28 days. Thirty Beagle dogs with similar characteristics and kept under the same management and diet were used. The measurement of dental plaque and calculus was performed using a computerized analytical method. The phenolic profile of KGB extract was analyzed by HPLC-DAD. KGB extract at 0.2% showed efficacy in controlling the formation of plaque compared to the negative control group, and dental calculus in relation to the negative and positive control groups. A significant difference was observed among these three groups. Peaks attributed to flavonoids and phenolic acids were identified in the HPLC-DAD chromatogram of the KGB extract. The presence of these substances could be related to the activity observed. Our findings demonstrate that treatment with KGB is effective in controlling periodontal disease in dogs, providing new insights into the medicinal properties of this plant. KGB extract has a potential use as a supplemental agent in pharmaceutical products for the prevention of periodontal disease. Efficacy of aINDEX TERMS: Kalanchoe gastonis-bonnieri, plant extract, bacterial biofilm, dental calculus, dogs, periodontal disease, dental plaque, flavonoids. TERMOS DE INDEXAÇÃO:Kalanchoe gastonis-bonnieri, extrato de plantas, biofilme bacteriano, cálculo dentário, cães, doença periodontal, cálculos dentários, placa bacteriana, flavonoides.

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“…Muitos estudos comprovam que os pacientes recebem pouca ou nenhuma informação quanto à INTRODUÇÃO higiene de suas próteses ou, muitas vezes, quando a recebem, estas informações são incompletas ou incorretas 7,8,9,10 . Desse modo, a atuação do profissional é decisiva tanto no tratamento protético propriamente dito bem como na instrução e motivação do paciente quanto aos seus deveres e cuidados em relação à sua prótese.…”

Section: Abstract: Oral Rehabilitation Removable Dentures Biofi Lmunclassified

Materiais E Métodos De Controle Do Biofilme Em Próteses Removíveis - Revisão De Literatura

Carvalho

Dantas²,

Lima³

2016

RevBahianaOdonto

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A atuação do dentista é decisiva tanto no tratamento reabilitador bem como na instrução e motivação do paciente quanto à higienização das próteses no momento de sua instalação e durante todo o seu uso. Cabe ao cirurgião-dentista conhecer os materiais e métodos empregados na manutenção da higiene bucal, selecioná-los e, de acordo com as peculiaridades de cada caso, motivar e orientar sua aplicação. Entretanto, o que se observa é uma difi culdade dos profi ssionais em utilizar um protocolo de controle de biofi lme na reabilitação bucal com próteses removíveis. O objetivo deste trabalho foi revisar a literatura acerca dos materiais e métodos disponíveis para a correta higiene das próteses removíveis. PALAVRAS-CHAVE A B S T R A C TThe role of the dentist is crucial both in rehabilitation treatment as well as in education and motivation of the patient regarding the hygiene of the prosthesis at the time of installation and throughout its use. It is important for the dentist to know the materials and methods used in maintaining oral hygiene, select them and motivate and guide their implementation according to the peculiarities of each case. However, it has been observed a diffi culty of professionals to use a biofi lm control protocol in oral rehabilitation with removable dentures. The objective of this study was to review the literature about the materials and methods available for the proper care of dentures.

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Evidenciadores de biofilme em prótese total: avaliação clínica e antimicrobiana

Cited by 29 publications

References 16 publications

Comparative study of methods for the quantification of biofilm on complete dentures

Comparative study of methods for the quantification of biofilm on complete dentures

Efficacy of a Kalanchoe gastonis-bonnieri extract to control bacterial biofilms and dental calculus in dogs

Materiais E Métodos De Controle Do Biofilme Em Próteses Removíveis - Revisão De Literatura

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