Characterization of diesel degrading bacterial species from contaminated tropical ecosystem

Nwinyi, Obinna; Kanu, Ijeoma Akudo; Tunde, Ayano; Ajanaku, K. O.

doi:10.1590/s1516-8913201402250

Cited by 28 publications

(18 citation statements)

References 28 publications

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“…Regarding the diesel biodegradation, bacteria exhibited growth, monitored by optical density with maximum values between 0.2 and 0.6, as depicted in Figure 2. This result was similar to that described by Nwinyi et al (2014), using bacteria belonging to the same genus displayed the ability to degrade diesel. However, the bacterial species growth decrease in diesel compared to the use of petroleum the carbon source can be attributed to the hydrocarbon stress on cell membranes, affecting the permeability and, consequently, the energy production.…”

Section: Resultssupporting

confidence: 91%

Petroleum hydrocarbon degradation by isolated mangrove bacteria

et al. 2018

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Los hidrocarburos de petróleo representan un problema mundial, pues su acumulación promueve un serio impacto ambiental. Así, el uso de microorganismos, por ejemplo los de la microbiota de manglares, como agentes degradadores de diversas fuentes de carbono, es poco explotado en procesos de remediación ambiental. Así, este estudio evaluó in vitro el potencial degradador de bacterias aisladas de sedimento de manglar en la degradación de hidrocarburos. El análisis genético usando el marcador 16S rRNA reveló secuencias íntimamente relacionadas (99%) con Proteobacterium, Pseudomonas y Exiguobacterium. Los resultados mostraron el crecimiento de bacterias en medio salino mineral (MSM) conteniendo petróleo o diesel al 1%, como fuentes de carbono. Este crecimiento, determinado por densidad óptica (DO) a 595 nm durante 15 días, con toma de muestras a cada 48 h, indicó la matabolización de hidrocarburos. Sin embargo, las bacterias fueron más eficientes en degradarlos. Por lo tanto, los resultados muestran la potencial aplicación de las bacterias en procesos de biorremediación por su capacidad metabólica y adaptativa de crecimiento usando hidrocarburos.

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Section: Resultssupporting

confidence: 91%

Petroleum hydrocarbon degradation by isolated mangrove bacteria

et al. 2018

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“…A single colony of each of the isolates was inoculated into 10 ml of nutrient broth incubated at 30 Cº for 24 h, centrifuged for 15 minutes at 3500 rpm, the cell pellet was washed twice with Bushnell-Hass medium (containing of g/l: magnesium sulfate: 0.2, calcium chloride: 0.02, monopotassium phosphate: 1, diammonium hydrogen phosphate: 1, potassium nitrate: 1, ferric chloride: 0.05, pH 7.0 ± 0.2) until optical density at 600 nm (OD600) was equivalent to 0.4 by using Spectrophotometer (Vis-7220. UK) [3,[34][35][36].…”

Section: Inoculum Preparationmentioning

confidence: 99%

“…Two ml of bacterial inoculum (OD600 = 0.4) were transferred into tubes containing Bushnell-Hass medium (8 mL) supplemented with crude oil (1, 2 and 3% v/v) as the only carbon source [36]. They were incubated at 30°C at 150 rpm for 15 days [3,24].…”

Section: Detection Of Ability Strains Of Micrococcus To Bioremediatiomentioning

confidence: 99%

“…This can be explained by the composition of organic acids [34,47]. However, the pH value showed a slight rise in concentration of 3%, and this was mentioned in some reference research [36,52]. This can be explained by the ability of bacteria to develop different methods of metabolism of hydrocarbons, or as a result of an exceptional reaction by the bacteria when used high concentration of crude oil [36].…”

Section: Biodegradation Of Crude Oilmentioning

confidence: 99%

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Evaluation Ability of Different Barada River Micrococcus Spp. Strain to Bioremediation of Hydrocarbons

Alsulaiman¹,

Nizam²

2018

J. CleanWAS

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The importance of this study is that it is deals with the Barada River pollution with hydrocarbons (crude oil and its derivatives), as well as evaluating the ability of different types of Micrococcus sp. to bioremediation of hydrocarbons. Ten strains of Micrococcus sp. were isolated from the water samples from Barada River in Damascus, and these isolated were classified depending on Bergey's manual to four species: M. luteus, M. lylae, M. flavus, and M. antarcticus. The crude oil dependent growths of these isolates were assessed for 15 days at 30°C by monitoring the gradient fluxes in pH, optical density OD and total viable count TVC in the medium. The result was statistically supported by applying the One-way ANOVA test and the Paired sample t-test. The results showed that activity of species was best at the concentration of 2% of crude oil, and the species of M. lylae was best in dealing with crude oil, although this superiority between species was not supported at the statistical studying. In general, the different species of Micrococcus sp. have a good ability to deal with the different concentrations (1, 2 and 3%) of crude oil. And this supports research which encourages the use of this genus in the bioremediation. This research was indicated to the ability of M. lylae to deal with different concentrations of crude oil, which was not mentioned in previous research KEYWORDS

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“…They are gram-negative, facultative, non-motile, usually encapsulated rod-shaped bacteria belonging to the family Enterobacteriaceae (Niemelä and Väätänen, 1982). These are often found in pristine and hydrocarbon polluted sites, such that they can degrade the heavy metals tolerable to them and the hydrocarbons as well (Kumaran et al, 2011;Nwinyi et al, 2014;Rodrigues et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Heavy metals bioremediation potential of Klebsiella species isolated from diesel polluted soil

Aransiola¹,

Ige²,

Ehinmitola³

et al. 2017

Afr. J. Biotechnol.

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Characterization of diesel degrading bacterial species from contaminated tropical ecosystem

Abstract: The bacterial diversity in a diesel contaminated tropical soil was investigated using diesel oxidation in gradient cultures dynamics (pH and OD)

Cited by 28 publications

References 28 publications

Petroleum hydrocarbon degradation by isolated mangrove bacteria

Petroleum hydrocarbon degradation by isolated mangrove bacteria

Evaluation Ability of Different Barada River Micrococcus Spp. Strain to Bioremediation of Hydrocarbons

Heavy metals bioremediation potential of Klebsiella species isolated from diesel polluted soil

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