2014
DOI: 10.1590/s1516-89132014000100004
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Abstract: Stevia rebaudiana Bertoni is a medicinal plants and commercially use as non-caloric sweetener for diabetic patient. In the present study, a protocol was developed for in vitro micropropagation using 6-benzylamino purine (BAP) and Kinetin (Kn) for the formation of multiple shoot proliferation and Indole-3-acetic acid (IAA), Indole-3-butyric acid (IBA) and 1-Naphthaleneacetic acid (NAA) for the induction of roots. Maximum shoot formation (7.82 ± 0.7 shoots per explants) was observed on a Murashige and Skoog (MS)… Show more

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Cited by 31 publications
(16 citation statements)
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“…68 -72 BAP 2 mg L −1 in combination with IAA 1.13 mg −1 showed 39 shoots per explant, 73 compared with BAP 0.5 mg L −1 in combination with kinetin 0.25 mg L −1 , with 7.82 shoots per explant. 74 Khalil et al 66 observed the best callus induction (84.6%) of stevia plantlet production on MS medium augmented with BA and 2,4-D (2.0 mg L −1 ). Highest frequency of rooting (96%) was noted on half-strength MS medium augmented with 0.4 mg L −1 NAA.…”
Section: Production Of Stevia Plants Through Tissue Culturementioning
confidence: 98%
See 1 more Smart Citation
“…68 -72 BAP 2 mg L −1 in combination with IAA 1.13 mg −1 showed 39 shoots per explant, 73 compared with BAP 0.5 mg L −1 in combination with kinetin 0.25 mg L −1 , with 7.82 shoots per explant. 74 Khalil et al 66 observed the best callus induction (84.6%) of stevia plantlet production on MS medium augmented with BA and 2,4-D (2.0 mg L −1 ). Highest frequency of rooting (96%) was noted on half-strength MS medium augmented with 0.4 mg L −1 NAA.…”
Section: Production Of Stevia Plants Through Tissue Culturementioning
confidence: 98%
“…A lot of work has been reported on in vitro propagation of stevia on media such as benzylaminopurine (BAP; 3 mg L −1 ), 2,4‐dichlorophenoxyacetic acid (2,4‐D; 1 mg L −1 ), NAA (10.74 µmol L −1 ), 6‐benzyladenine (BA; 1 mg L −1 ) and IAA (1 mg L −1 ), showing maximum shoots/explant . BAP 2 mg L −1 in combination with IAA 1.13 mg −1 showed 39 shoots per explant, compared with BAP 0.5 mg L −1 in combination with kinetin 0.25 mg L −1 , with 7.82 shoots per explant . Khalil et al observed the best callus induction (84.6%) of stevia plantlet production on MS medium augmented with BA and 2,4‐D (2.0 mg L −1 ).…”
Section: Agrotechniquesmentioning
confidence: 99%
“…Berdasarkan hasil penelitian menunjukan bahwa penggunaan zat pengatur tumbuh Laribi et al (2012) menyatakan bahwa induksi perakaran mencapai 97%, jumlah akar 8,90 dan panjang akar 1,70 cm dengan menggunakan IAA 0,5 mg/L. Razak et al (2014) menambahkan bahwa panjang akar terbaik diperoleh pada media dasar MS dengan penambahan IAA 0,5 mg/L dengan panjang akar 5,40 cm. Auksin sangat diperlukan dalam pembentukan akar yaitu untuk memacu terjadinya pembelahan sel.…”
Section: Induksi Perakaranunclassified
“…Stevia is usually propagated by the conventional method, but this method was inefficient due to the low viability of its seeds due to self incompatibility (Ramírez and Iglesias, 2016), large variability in levels and composition of stevioside (Anbazhagan et al, 2010), and less than 10 % germination rates (Razak et al, 2014). In vitro method provides an efficient way to tackle these problems and this method is capable of growing a large number of stevia plants and less time consuming compared with the conventional methods.…”
Section: Introductionmentioning
confidence: 99%