Properties of a biosurfactant produced by Bacillus pumilus using vinasse and waste frying oil as alternative carbon sources

The use of efficient green cleaning agents, such as biosurfactants, is important in oil sludge treatment.Enhanced oil recovery from oily sludge by different rhamnolipids was comparatively evaluated. Using crude glycerol, the wild-type strain Pseudomonas aeruginosa SG and the recombinant strains P.aeruginosa PrhlAB and P. stutzeri Rhl produced 1.98 g L À1 , 2.87 g L À1 and 0.87 g L À1 of rhamnolipids, respectively. The three bacterial strains produced different rhamnolipid mixtures under the same conditions. The proportions of mono-rhamnolipids in the three rhamnolipid products were 55.92%, 94.92% and 100%, respectively. These rhamnolipid products also possessed different bioactivities.Emulsifying activity became higher as the proportion of mono-rhamnolipids increased. The three rhamnolipid products were stable at temperatures lower than 121 C, pH values from 5-11 and NaCl concentrations from 0-15%. All three rhamnolipid products could recover oil from oily sludge, but oil recovery efficiency was positively related to the proportion of mono-rhamnolipids. Mono-rhamnolipids produced by the recombinant strain Rhl exhibited the best oil recovery efficiency (53.81%). The results reveal that mono-rhamnolipids are the most promising for oil recovery from oily sludge.

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“…The CMC of SDS is 2100 mg L À1 . 32,33 Compared with synthetic surfactants, the three rhamnolipid products are highly excellent.…”

Section: Emulsifying Activity Of the Three Rhamnolipid Productsmentioning

confidence: 99%

Production of rhamnolipids with different proportions of mono-rhamnolipids using crude glycerol and a comparison of their application potential for oil recovery from oily sludge

et al. 2019

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“…This demonstrates the excellent stability of the microorganism, which is one of the desirable requirements for microorganisms used in industry (Schmidell et al, ). In addition, B. pumilus produced greater efficiency in the reduction of surface tension when compared to other studies that used the same bacterium (Calvo et al, ; Fooladi et al, ; Oliveira and Garcia‐Cruz, ; Sharma et al, ).…”

Section: Resultsmentioning

confidence: 61%

Biosurfactants Production Using Permeate from Whey Ultrafiltration and Bioproduct Recovery by Membrane Separation Process

Decesaro

Machado

Cappellaro

et al. 2020

J Surfact & Detergents

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The management of whey is a challenge for dairy products where the volume produced is remarkable. This problem is minimized through membrane separation processes (MSP) to obtain whey protein concentrate, which has high added value. However, a permeate effluent stream is still generated that is composed of lactose, vitamins, and minerals, which can serve as raw material for the production of biotechnological compounds. Thus, this study aimed to produce biosurfactants using the permeate from whey ultrafiltration as part of the culture media of the bioprocess, to recover the biosurfactant produced using MSP, and to identify the biocompound. The production was carried out using Bacillus methylotrophicus and Bacillus pumilus. The variables nitrogen source (urea or ammonium sulfate), nitrogen source concentration (0.5% or 1.0%), inducer (soybean oil or biodiesel), inducer concentration (1% or 2%), and the addition of micronutrients (with our without) were studied using a fractional factorial experimental design 2 5-1 IV . In the fermentation processes, it was possible to verify the biosurfactant production through the reduction of surface tension, obtaining a minimum value of 35.07 mN/m for B. methylotrophicus and 26.02 mN/m for B. pumilus. Recovery via MSP was an efficient strategy for biosurfactant purification, which was concentrated in the fraction of the retentate. We produced a high-value-added biocompound identified as surfactin, valuing the permeate residue from whey ultrafiltration.Results of mean AE standard deviation. Equal letters in the same column indicate that they did not present a significant difference at the 95% confidence level. RBC, relation between final and initial biomass concentration; RST, relation between initial and final surface tension; T, treatment. J Surfact DetergJ Surfact Deterg (2020) 23: 539-551Equal letters in the same column and for each microorganism indicate that they did not present a significant difference at the 95% confidence level. MSP, membrane separation processes. 547 J Surfact Deterg J Surfact Deterg (2020) 23: 539-551

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“…Haddad et al [37] found that biosurfactat production by B. subtilis HOB2 was growthassociated, since the strain produced surfactant within 24 h of incubation. However, in the study of Oliveira and Garcia-Cruz [38] with B. pumilus, the biosurfactant biosynthesis in medium containing vinasse or frying oil as carbon source, was not growth-associated.…”

Section: Discussionmentioning

confidence: 87%

Biosurfactant Production by Bacillus strains isolated from sugar cane mill wastewaters

Santos

Miranda²,

Silva

et al. 2019

Braz. arch. biol. technol.

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Biosurfactants possess diverse chemical properties and provide important characteristics to the producing microorganisms, which can act as surface-actives and emulsifiers of hydrocarbon and others water insoluble substances. Most of them are lipopeptides synthetized by Bacillus. This study evaluated the biosurfactant production by strains of Bacillus previously obtained from liquid residues of sugar-alcohol industry. The bacterial isolates LBPMA: BSC, BSD, J1, J2 and L1 were cultivated in medium that induces production of biosurfactants (Landy medium). During 48 h of incubation, at intervals of 12 h, the total contents of proteins, reducing carbohydrates and surfactant activity of the filtrated growth media free of cells were evaluated. The results showed that these strains use glucose as a source of carbon, energy and for synthesis of surfactant. In this medium (24 h), the best producer of biosurfactant was the strain LBPMA-J2, molecularly identified HIGHLIGHTS • Interest in biosurfactants has increased in the last two decades. • Surfactin from Bacillus is one of the most effective biosurfactants. • Bacillus species are known as the main producers of extracellular enzymes. • Isolation of new strains is strategic for several fields of biotechnology. Santos, E.C.L.; et al.

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Properties of a biosurfactant produced by Bacillus pumilus using vinasse and waste frying oil as alternative carbon sources

Abstract: ABSTRACT

Cited by 49 publications

References 25 publications

Production of rhamnolipids with different proportions of mono-rhamnolipids using crude glycerol and a comparison of their application potential for oil recovery from oily sludge

Production of rhamnolipids with different proportions of mono-rhamnolipids using crude glycerol and a comparison of their application potential for oil recovery from oily sludge

Biosurfactants Production Using Permeate from Whey Ultrafiltration and Bioproduct Recovery by Membrane Separation Process

Biosurfactant Production by Bacillus strains isolated from sugar cane mill wastewaters

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