Changes in diapause related gene expression pattern during early embryonic development in HCl-treated eggs of bivoltine silkworm Bombyx mori (Lepidoptera: Bombycidae)

Sasibhushan, Sirigineedi; Ponnuvel, Kangayam M.; Vijayaprakash, N. B.

doi:10.1590/s1516-89132013000100001

Cited by 6 publications

(4 citation statements)

References 32 publications

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“…Compared with the diapause egg, non-diapause eggs displayed higher sorbitol dehydrogenase activity during early embryonic development [24]. Temperature stress and HCl treatment can increase the expression of sorbitol dehydrogenase [13,25]. In the present study, two sorbitol dehydrogenases (BGIBMGA012399 and BGIBMGA012400) were significantly upregulated at 12 h after the O 2 treatment (Table S8), but not after the HCl treatment, compared with the control.…”

Section: Discussionmentioning

confidence: 48%

“…In order to investigate the mechanism of embryonic diapause in silkworm, large-scale screenings were performed, including genome-wide microarray and shotgun liquid chromatography–tandem mass spectrometry, combined with bioinformatics, to identify differentially expressed genes (DEGs) in diapause and non-diapause eggs [13,14,15]. Our goal was to study the underlying mechanism of diapause and diapause blocking by treating diapause-destined eggs with O 2 or HCl.…”

Section: Introductionmentioning

confidence: 99%

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Transcriptional Response of Silkworm (Bombyx mori) Eggs to O2 or HCl Treatment

Gong

Tian

Zhou

et al. 2016

IJMS

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Diapause is a common biological phenomenon that occurs in many organisms, including fish, insects, and nematodes. In the silkworm (Bombyx mori), diapause generally occurs in the egg stage. Treatment with O2, HCl, or other compounds can prevent egg diapause. Here, we characterized the transcriptomic responses of newly laid eggs treated with O2 or HCl. Digital gene expression analysis showed that 610 genes in O2-treated eggs and 656 in HCl-treated eggs were differentially expressed. Of these, 343 genes were differentially expressed in both treatments. In addition to trehalases, sorbic acid dehydrogenases, and some enzymes involved in the carbohydrate metabolism, we also identified heat shock proteins, cytochrome P450, and GADD45, which are related to stress tolerance. Gene ontology enrichment analysis showed differentially expressed genes in O2-treated eggs were involved in oxidoreductase activity as well as in binding, catalytic, and metabolic processes. The Kyoto Encyclopedia of Genes and Genomes analysis showed that the pathways for ribosome biogenesis, spliceosome, and circadian rhythm were significantly enriched in HCl-treated eggs. The reliability of the data was confirmed by qRT-PCR analysis. Our results improved the understanding of the mechanism of diapause blocking in silkworm eggs treated with O2 or HCl and identified novel molecular targets for future studies.

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Section: Discussionmentioning

confidence: 48%

Section: Introductionmentioning

confidence: 99%

Transcriptional Response of Silkworm (Bombyx mori) Eggs to O2 or HCl Treatment

Gong

Tian

Zhou

et al. 2016

IJMS

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“…Hsps have been found to be involved in many developmental processes, including insect diapause, metamorphosis, embryogenesis, apoptosis, and autophagy ( Chu et al, 2020 ). Hsp synthesis in insects is differently regulated not only at series of developmental stages but also during the diapause process ( Sasibhushan et al, 2013 ). The expression levels of Hsp70 were greatly increased in diapausing pupae of the blueberry maggot, Rhagoletis mendax ( Teixeira and Polavarapu, 2005 ).…”

Section: Introductionmentioning

confidence: 99%

Characterization, and Functional Analysis of Hsp70 and Hsp90 Gene Families in Glyphodes pyloalis Walker (Lepidoptera: Pyralidae)

et al. 2021

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Glyphodes pyloalis Walker (Lepidoptera: Pyralidae) is a destructive mulberry pest, causing great damage to mulberry in China. Heat shock proteins (Hsps) are involved in various signal pathways and regulate lots of physiological processes in insects. The function of Hsps in G. pyloalis, however, has still received less attention. Here, we identified five Hsp genes from G. pyloalis transcriptome dataset including two Hsp70 family genes (GpHsp71.3 and GpHsp74.9) and three Hsp90 family genes (GpHsp82.4, GpHsp89, and GpHsp93.4). Quantitative Real-time PCR validation revealed that all Hsps of G. pyloalis have significant expression in pupal and diapause stage, at which the larvae arrest the development. Expressions of GpHsp71.3 and GpHsp82.4 were increased significantly after thermal treatment at 40°C, and this upregulation depended on heat treatment duration. Furthermore, silencing GpHsp82.4 by RNA interference led to a significant increase in mortality of G. pyloalis larvae under the heat stress compared to the control group. After starvation stress, the expression levels of GpHsp82.4 and GpHsp93.4 were significantly increased. At last, after being parasitized by the parasitoid wasp Aulacocentrum confusum, Hsp70 and Hsp90 genes of G. pyloalis were decreased significantly in the early stage of parasitization and this moderation was affected by time post-parasitization. This study highlights the function of G. pyloalis Hsps in response to environmental stress and provides a perspective for the control of this pest.

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“…As widely known that the continuous division and proliferation of embryonic cells is the key to break silkworm diapause, however,the molecular mechanisms of diapause is unclear. With the next-generation sequencing technology, researchers began to use high-throughput screening approach to determine the differentially expressed genes of diapause and non-diapause eggs on the transcriptomics [ 9 , 10 ] and proteomic levels[ 11 , 12 ] to study bombyx embryonic development.…”

Section: Introductionmentioning

confidence: 99%

Differentially expressed microRNAs in diapausing versus HCl-treated Bombyx embryos

et al. 2017

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Differentially expressed microRNAs were detected to explore the molecular mechanisms of diapause termination. The total small RNA of diapause-destined silkworm eggs and HCl-treated eggs was extracted and then sequenced using HiSeq high-throughput method. 44 novel miRNAs were discovered. Compared to those in the diapause-destined eggs, 61 miRNAs showed significant changes in the acid-treated eggs, with 23 being up-regulated and 38 being down-regulated. The potential target genes of differentially expressed miRNAs were predicted by miRanda. Gene Ontology and KEGG pathway enrichment analysis of these potential target genes revealed that they were mainly located within cells and organelles, involved in cellular and metabolic processes, and participated in protein production, processing and transportation. Two differentially expressed genes, Bombyx mori SDH and Bmo-miR-2761-3p, were further analyzed with qRT-PCR. BmSDH was significantly up-regulated in the HCl-treated eggs, while Bmo-miR-2761-3p was down-regulated. These results suggested that these two genes were well coordinated in silkworm eggs. Dual luciferase reporter assay demonstrated that Bmo-miR-2761-3p inhibited the expression of BmSDH.

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Changes in diapause related gene expression pattern during early embryonic development in HCl-treated eggs of bivoltine silkworm Bombyx mori (Lepidoptera: Bombycidae)

Abstract: Investigation of differential expression of diapause related genes (five metabolic, five heat shock protein and one translational regulatory) in HCl-treated (non-diapause)

Cited by 6 publications

References 32 publications

Transcriptional Response of Silkworm (Bombyx mori) Eggs to O2 or HCl Treatment

Transcriptional Response of Silkworm (Bombyx mori) Eggs to O2 or HCl Treatment

Characterization, and Functional Analysis of Hsp70 and Hsp90 Gene Families in Glyphodes pyloalis Walker (Lepidoptera: Pyralidae)

Differentially expressed microRNAs in diapausing versus HCl-treated Bombyx embryos

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