Selection of cellulolytic fungi isolated from diverse substrates

Damaso, M. C. T.; Terzi, Selma da Costa; Farias, A. X. de; Oliveira, Andréa C.; Fraga, Marcelo Elias; Couri, Sônia

doi:10.1590/s1516-89132012000400005

Cited by 19 publications

(14 citation statements)

References 23 publications

(15 reference statements)

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“…There have been abundant reports during the last decade about biological fungicides by solid substrates and optimizing production procedures (Pascual et al 1999;Budge and Whipps 2001;Schuster and Schmoll 2010;Damaso et al 2012;Sargin et al 2013). For instance, Pascual et al (1999) showed the solid biological fungicide containing the fungus Epicoccum nigrum on wheat.…”

Section: Introductionmentioning

confidence: 99%

Effectiveness of the chemical stabilizers of Talaromyces flavus in biological control of tomato and greenhouse cucumber vascular wilt disease

Bahramian¹,

Naraghi²,

Heydari³

2016

Journal of Plant Protection Research

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Fungal antagonist, Talaromyces flavus, is one of the most important biological agents of soil-borne fungal diseases including Verticillium and Fusarium wilt. In this study, to increase the effectiveness of T. flavus isolates obtained from greenhouse cucumbers and field grown tomatoes five chemical stabilizers were evaluated. Based on the results of previous studies, the most effective substrate for the growth, sporulation and stability of T. flavus isolates related to the above-mentioned plants was a mix of rice bran and peatmoss. Different chemical stabilizers were mixed with the above-mentioned substrate containing spore suspensions of various T. flavus isolates. For each plant, a completely randomized experiment was conducted under greenhouse conditions with seven treatments and three replications. The results of this study indicated that treatments containing sodium nitrate and D-cycloserine were more effective than those containing other stabilizers. The overall results of this study suggest that the use of some chemical stabilizers may enhance the biocontrol potential of fungal antagonists in controlling different plant diseases including Verticillium and Fusarium wilt.

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Section: Introductionmentioning

confidence: 99%

Effectiveness of the chemical stabilizers of Talaromyces flavus in biological control of tomato and greenhouse cucumber vascular wilt disease

Bahramian¹,

Naraghi²,

Heydari³

2016

Journal of Plant Protection Research

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“…A large number of carboxymethyl functional groups (the degree of substitution: 0.65-0.90) suppresses the exoglucanase (EXG) activity which could The mean values given in columns with different letter index are significantly different (α < 0.05); Group 1 -selected isolates which had the highest RS values while grown under submerged fermentation (SmF) with 1.0% CMC; Group 2 -selected isolates which had the highest EI values while grown on solid state media with CMC; Significant differences were determined separately for group 1 and group 2; X -unidentified fungus affect either the RS amount or the hydrolysis degree (Zhang et al, 2006). For EI determination, Damaso et al (2012) and Goldbeck et al (2012) used celluloses other than CMC, e.g., microcrystalline cellulose: Avicel or Servacel. For cellulase activity determination, at the next selection stages, various cellulose types were applied: Servacel (Goldbeck et al, 2012); Whatman No.…”

Section: Application Of Cmc As a Substratementioning

confidence: 99%

“…For cellulase activity determination, at the next selection stages, various cellulose types were applied: Servacel (Goldbeck et al, 2012); Whatman No. 1 filter paper (Ruegger and Tauk-Tornisielo, 2004;Jahangeer et al, 2005;Florencio et al, 2012); and wheat bran (Ruegger and Tauk-Tornisielo, 2004;Damaso et al, 2012;Florencio et al, 2012). Application of cellulose with different physicochemical properties can affect the activity of cellulases.…”

Section: Application Of Cmc As a Substratementioning

confidence: 99%

“…At this stage, only few samples are subjected to a quantitative evaluation. Sazci et al (1986) and Florencio et al (2012) showed a correlation between the results obtained by semiquantitative and quantitative methods, but Goldbeck et al (2012) and Damaso et al (2012) pointed out that EI could not discern between isolates with different cellulase activities.…”

Section: Introductionmentioning

confidence: 96%

“…At the second stage, the isolates with no cellulose hydrolysis zone around the colony (then EI values cannot be calculated) (Jahangeer et al, 2005;Goldbeck et al, 2012) as well as those whose EI is below a predefined value are rejected. Damaso et al (2012) set the threshold to 1.0, Florencio et al (2012) to 1.5, and Lopes et al (2011) to 2.5. At this stage of semiquantitative appraisal, some Trichoderma species with no EI determined but with the highest EG activity can be omitted (Ruegger and Tauk-Tornisielo, 2004).…”

Section: Introductionmentioning

confidence: 99%

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Application of semi-quantitative and quantitative methods for the selection of cellulolytic filamentous fungi isolated from pulp mill materials

Miklaszewska¹,

Macko²,

Kłosowski³

et al. 2016

bta

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A semi-quantitative method (the enzymatic activity index, EI) and a quantitative method (the amount of reducing sugars, RS) have been used for selecting cellulolytic microorganisms. The analysis was performed with 90 isolates of filamentous fungi selected from pulp mill materials. In both methods 1% low viscosity carboxymethylcellulose (1% CMC l.v.) was used as a substrate. For n = 90, no correlation between the parameters has been found.A further analysis was carried out for 12 selected isolates: 6 with the highest RS values and 6 with the highest EI values. For the chosen fungi, also cultivated on 1% CMC l.v. medium, the following parameters have been determined: the total cellulase activity (filter paper assay, FPase), endoglucanase (CMCase) activity, β -glucosidase (CBase) activity, and the changes in the culture medium viscosity (DV). The statistical analysis revealed a negative, moderately marked correlation between EI and RS and clearly marked positive correlations for two pairs: RS-FPase and DV-FPase. A high positive correlation between RS and DV has also been observed. Correlations between EI, RS, DV, CMCase, and CBase activities were weak. It was shown that the quantitative parameter RS is more suitable than EI for the evaluation of the total cellulolytic activity of filamentous fungi. The application of CMC as the only source of carbon in the culture media and the subsequent determination of EI or RS has failed to provide the means for selecting fungi with a high endoglucanase (CMCase) activity.

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Isolation and cultivation of xylanolytic and cellulolytic Sarocladium kiliense and Trichoderma virens from the gut of the termite Reticulitermes santonensis

Tarayre

Bauwens

Brasseur

et al. 2014

Environ Sci Pollut Res

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The purpose of this work was the isolation and cultivation of cellulolytic and xylanolytic microorganisms extracted from the gut of the lower termite Reticulitermes santonensis. Microcrystalline cellulose (with and without lignin) and beech wood xylan were used as diets instead of poplar wood in order to select cellulose and hemicellulose-degrading fungi. The strain Sarocladium kiliense (Acremonium kiliense) CTGxxyl was isolated from the termites fed on xylan, while the strain Trichoderma virens CTGxAviL was isolated from the termites fed on cellulose (with and without lignin). Both molds were cultivated in liquid media containing different substrates: agro-residues or purified polymers. S. kiliense produced maximal β-glucosidase, endo-1,4-β-D-glucanase, exo-1,4-β-D-glucanase and endo-1,4-β-D-xylanase activities of 0.103, 3.99, 0.53, and 40.8 IU/ml, respectively. T. virens produced maximal β-xylosidase, endo-1,4-β-D-glucanase, exo-1,4-β-D-glucanase, and endo-1,4-β-D-xylanase activities of 0.38, 1.48, 0.69, and 426 IU/ml. The cellulase and the xylanase of S. kiliense, less common than T. virens, were further investigated. The optimal activity of the xylanase was observed at pH 9-10 at 60 °C. The cellulase showed its maximal activity at pH 10, 70 °C. Zymography identified different xylanases produced by both molds, and some fragment sizes were highlighted: 35, 100, and 170 kDa for S. kiliense and 20, 40, 80, and 170 kDa for T. virens. In both cases, endo-1,4-β-D-xylanase activities were confirmed through mass spectrometry.

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Selection of cellulolytic fungi isolated from diverse substrates

Abstract: ABSTRACT

Cited by 19 publications

References 23 publications

Effectiveness of the chemical stabilizers of Talaromyces flavus in biological control of tomato and greenhouse cucumber vascular wilt disease

Effectiveness of the chemical stabilizers of Talaromyces flavus in biological control of tomato and greenhouse cucumber vascular wilt disease

Application of semi-quantitative and quantitative methods for the selection of cellulolytic filamentous fungi isolated from pulp mill materials

Isolation and cultivation of xylanolytic and cellulolytic Sarocladium kiliense and Trichoderma virens from the gut of the termite Reticulitermes santonensis

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