Detection of Lsr2 gene of Mycobacterium leprae in nasal mucus

Custodio, Luiz Antonio; Saito, Alexandre Yukio; Amarante, Marla Karine; Fujita, Tomoo; Perim, Aparecida de Lourdes; Costa, Ivete Conchon; Felipe, Ionice; Jankevicius, Shiduca Itow

doi:10.1590/s1516-89132012000300007

Cited by 3 publications

(3 citation statements)

References 22 publications

(6 reference statements)

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“…1 ). Despite the lower PCR positivity in EG, levels reported are higher than published previously ( de Almeida et al 2004 , Nagao-Dias et al 2007 , Banerjee et al 2010 , Custodio et al 2012 ). Moreover, studies indicate that contacts of leprosy cases that are nasal PCR positive have a higher risk of developing the disease ( Douglas et al 2004 , Bakker et al 2006 ).…”

Section: Discussioncontrasting

confidence: 73%

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Widespread nasal carriage of Mycobacterium lepraeamong a healthy population in a hyperendemic region of northeastern Brazil

Lima

Frota

Mota

et al. 2015

Mem. Inst. Oswaldo Cruz

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A case-control study was conducted to determine the presence of Mycobacterium leprae DNA in nasal secretions of leprosy cases and nonleprosy individuals in Fortaleza, Brazil. It included 185 cases identified by physicians at the Dona Libânia National Reference Centre for Sanitary Dermatology (CDERM). A control group (Co) (n = 136) was identified among individuals from CDERM not diagnosed as leprosy cases. To augment the spatial analysis of M. leprae specific repetitive element (RLEP) positive prevalence, an external group (EG) (n = 121), a convenience sample of healthy students, were included. Polymerase chain reaction for the RLEP sequence was conducted for all participants. Prevalence of RLEP positivity for cases and Co were 69.2% and 66.9%, respectively, significantly higher than for EG (28.1%), and reported elsewhere. Male sex, belonging to a lower socioeconomic status (D/E), history of a previous contact with a case and being older, were associated with being a leprosy case. Our geographical analysis demonstrated that the bacillus is widespread among the healthy population, with clusters of RLEP positive multibacillary cases concentrated in distinct areas of the city. Our results suggest that in endemic areas, as in Fortaleza, surveillance for both nonhousehold leprosy contacts and members of the general population living in cluster areas should be implemented.

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Section: Discussioncontrasting

confidence: 73%

“…Detection of the bacilli may be demonstrated in studies among contacts showing rates of positivity ranging from 1.7-23% ( de Almeida et al 2004 , Nagao-Dias et al. 2007 , Banerjee et al 2010 , Custodio et al 2012 ).…”

Section: Discussionmentioning

confidence: 99%

Widespread nasal carriage of Mycobacterium lepraeamong a healthy population in a hyperendemic region of northeastern Brazil

Lima

Frota

Mota

et al. 2015

Mem. Inst. Oswaldo Cruz

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“…for detecting a very small number of genomic templates in a clinical sample. Several specific genomic regions have been targeted such as 16S rRNA (7) rpoT and sodA (18,19) 36-kDa antigen (20), Complex 85 (21) and the repetitive sequences (LEPRPT, LEPREP, REPLEP and RLEP) ( 22) among other M. leprae genes (23)(24)(25) of which the most sensitive and specific sequence was found to be RLEP region (26). Other repeated loci can also be targeted that are present in fewer copies.…”

Section: Diagnostic Tests Based On Nucleic Acid Amplification Tests (...mentioning

confidence: 99%

Advances in the Diagnosis of Leprosy

Sharma¹,

Singh²

2022

Front. Trop. Dis

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Leprosy is a public health issue, and early detection is critical to avert disability. Despite the global attempt to eradicate this disease as a public health problem, it remains an important cause of global neurological disability. India, Brazil and Indonesia share more than 70% of the cases. The reduction of new cases is a priority in the WHO global strategy 2021-2030 which aims to reduce disease transmission in the community by diagnosing cases and identifying subclinical infection. The clinical manifestations of leprosy range from a few to several lesions. The identification remains difficult due to the limited sensitivity of traditional approaches based on bacillary counts of skin smears and histology. To aid in the diagnosis of this disease, molecular biology, and biotechnological technologies have been applied, each with its own set of benefits and downsides despite providing an essential tool to validate the clinical diagnosis of leprosy. Because of this, it is strongly recognized that specific, inexpensive point of care technologies should be developed, particularly to identify asymptomatic M. leprae infections or leprosy nearer to the suspected cases seeking medical attention. Thus, this review will provide an overview of the advancements in leprosy diagnosis over the world. The purpose of this review is to improve our understanding of the outcomes of current tests and technologies used in leprosy diagnosis and to emphasize critical aspects concerning the detection of leprosy bacilli.

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