“…Biological Sciences Maringá, v. 38, n. 3, p. 283-289, July-Sept., 2016 The results obtained in the present study are similar or superior to those obtained in studies where defined substrates, such as sucrose (Kalil, Suzan, Maugeri, & Rodrigues, 2001;SilvaSantisteban & Maugeri, 2005) or commercial inulin (Kumar, Kunamneni, Prabhakar, & Ellaiah, 2005;Singh, Sooch, & Puri, 2007;Singh & Lotey, 2010) were used, which are more expensive than agave syrup. According to Silva-Santisteban, Converti, &Chen et al (2011), inulinase production can vary widely, because its biosynthesis depends on the used sources of carbon and nitrogen, on their medium concentration, as well as on the used microorganisms and strains.…”