2010
DOI: 10.1590/s1516-89132010000300024
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Abstract: This paper describes a protocol for the efficient vegetative propagation of

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Cited by 22 publications
(21 citation statements)
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“…This species has been studied using plant cell culture strategies (Simões et al 2004(Simões et al , 2009a, in relation to its medicinal potential (Simões et al 2006). More recently, an anthocyanin-producing callus culture line has also been obtained (Simões et al 2009b).…”
mentioning
confidence: 99%
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“…This species has been studied using plant cell culture strategies (Simões et al 2004(Simões et al , 2009a, in relation to its medicinal potential (Simões et al 2006). More recently, an anthocyanin-producing callus culture line has also been obtained (Simões et al 2009b).…”
mentioning
confidence: 99%
“…Stem segments (0.5 cm) from in vitro-grown plants (Simões et al 2004) were incubated on solidified Murashige and Skoog (MS) (1962) medium containing 30 g l -1 sucrose and supplemented with 0.90 lM 2,4-dichlorophenoxyacetic acid (2,4-D). Calli were subcultured using the same medium composition by transferring samples of approximately 4 g at 20-day intervals.…”
mentioning
confidence: 99%
“…Cleome rosea Vahl (Capparaceae) is a Brazilian herbaceous annual species frequently found in coastal sandy plains, known as ''restingas'', which are ecosystems intensely affected by human impact. This species can be used as garden ornamental, due to its inflorescences with beautiful and attractive pink flowers and has recently been studied in regard to biotechnological (Simões et al 2004) and medicinal (Simões et al 2006) aspects.…”
Section: Introductionmentioning
confidence: 99%
“…In vitro clonal propagation is only the alternative way for the regeneration and expedites release of large number of C. viscosa plants under aseptic culture conditions. Earlier, there have been few reports on establishment of in vitro propagation through callus culture of C. viscosa [8][9], micropropagation and shoot organogenesis from different explants of Cleome spinosa [10] - [13] and Cleome gynanadra [14], somatic embryogenesis [15] cell suspension and root culture with cryopreservation of C. rosea [16]. The present study was carried out to standardize a protocol for in vitro propagation of C. gynandra from nodal explants.…”
Section: Introductionmentioning
confidence: 99%