Solid State production of manganese peroxidases using arecanut husk as substrate

Rajan, A. R.; Kurup, Jayalakshmi Gopinatha; Abraham, T. Emilia

doi:10.1590/s1516-89132010000300008

Cited by 20 publications

(6 citation statements)

References 14 publications

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“…5b). (Yasmeen et al, 2011) have observed 4.5 optimum pH, (Asalam and Asgher 2011) 4, and (Rajan et al 2010) the optimum pH of 5 for MnP activity. An optimal pH range from 4 to 7 has been observed by (de Oliveira et al 2009, Baborova et al 2006, Bermek et al 2004.…”

Section: Optimization Of Temperature Of Ligninolytic Enzymesmentioning

confidence: 97%

Archives of Environmental Protection

Khan¹,

Asad²,

Mahmood³

et al. 2020

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Ligninolytic enzymes are employed for the production of second-generation biofuel to minimize fuel crisis. Additionally, they play a crucial role in global carbon cycle and a variety of applications in food, agriculture, paper and textile industries. On a large scale production of ligninolytic enzymes, microorganisms can be cultured on lignocellulosic wastes. In the present study, proximate analysis including acid detergent lignin (ADL), acid detergent cellulose (ADC), acid detergent fi ber (ADF) and acid insoluble ash (AIA) were performed for Platanus orientalis (chinar), Bauhinia variegata (orchid tree), Pinus roxburghii (chir pine), wheat straw and wheat husk. Platanus orientalis was selected as substrate because of higher lignin contents for the production of ligninolytic enzymes by Aspergillus fl avus. Solid State Fermentation was used and Response Surface Methodology was employed for optimizing various parameters and enzymes production. Maximum production was achieved at temperature 32°C, fermentation period 120 hours, pH 4.5, inoculums size 3.5 mL, substrate mesh size 80 mm, substrate size 7 g. Maximum purifi cation of laccase, manganese peroxidase (MnP) and lignin peroxidase (LiP) was achieved with 50%, 60% and 40% ammonium sulfate respectively and it was enhanced by gel fi ltration chromatography. Characterization of enzymes shows that Laccase has 35°C optimum temperature, 4.5 pH, 0.289 mM Km and 227.27 μM/ml Vmax. Manganese peroxidase has 30°C optimum temperature, 5.5 pH, 0.538 mM Km and 203.08 μM/ml Vmax. Lignin peroxidase has 30°C optimum temperature, 3 pH, 2 mM Km and 2000 uM/ml Vmax. Protein concentrations found in crude extracts and partially purifi ed enzymes are respectively: laccase 1.78 and 0.71 mg/ml, MnP 1.59 and 0.68 mg/ml. LiP, 1.70 and 0.69 mg/ml.

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Section: Optimization Of Temperature Of Ligninolytic Enzymesmentioning

confidence: 97%

Archives of Environmental Protection

Khan¹,

Asad²,

Mahmood³

et al. 2020

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“…Manganese dependent peroxidase is produced from P. pulmonarius, which can oxidize both non-phenolic and phenolic compounds for xenobiotic compound degradation. Kuhar and co-workers [264] have reported that MnCl 2 can induce MnP activity and has a high specificity for Mn 2+ binding sites.…”

Section: Manganese Peroxidasementioning

confidence: 99%

Cultivation of Mushrooms and Their Lignocellulolytic Enzyme Production Through the Utilization of Agro-Industrial Waste

et al. 2020

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A large amount of agro-industrial waste is produced worldwide in various agricultural sectors and by different food industries. The disposal and burning of this waste have created major global environmental problems. Agro-industrial waste mainly consists of cellulose, hemicellulose and lignin, all of which are collectively defined as lignocellulosic materials. This waste can serve as a suitable substrate in the solid-state fermentation process involving mushrooms. Mushrooms degrade lignocellulosic substrates through lignocellulosic enzyme production and utilize the degraded products to produce their fruiting bodies. Therefore, mushroom cultivation can be considered a prominent biotechnological process for the reduction and valorization of agro-industrial waste. Such waste is generated as a result of the eco-friendly conversion of low-value by-products into new resources that can be used to produce value-added products. Here, we have produced a brief review of the current findings through an overview of recently published literature. This overview has focused on the use of agro-industrial waste as a growth substrate for mushroom cultivation and lignocellulolytic enzyme production.

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“…Penambahan induser pada mikroorganisme berperan untuk mengaktifkan dan meningkatkan kerja enzim (Vrsanska et al, 2015). Hal ini didukung oleh hasil penelitian Rajan et al (2010), penambahan 0,1 mM MnSO4 pada P. chrysosporium mampu meningkatkan aktivitas enzim MnP hingga 54.03 IU/L. Jika dibandingkan, aktivitas enzim MnP pada penelitian ini lebih tinggi.…”

Section: Uji Kadar Lignoselulosaunclassified

PENGARUH PENAMBAHAN MnSO4 TERHADAP AKTIVITAS ENZIM MANGAN PEROKSIDASE PADA DELIGNIFIKASI LIMBAH BAGASSE OLEH PHLEBIA SP. MG-60

Nurika

Majid

Suprayogi

2019

JTP

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Kandungan lignin pada bagasse dapat dimanfaatkan sebagai bahan kimia bernilai tinggi melalui proses degradasi lignoselulosa. Phlebia sp. MG-60 merupakan salah satu jenis jamur pelapuk yang menghasilkan enzim mangan peroksidase (MnP) untuk mendegradasi lignin. Akan tetapi, untuk meningkatkan aktivitas enzim MnP dibutuhkan induser seperti MnSO4. Tujuan penelitian ini adalah untuk mengetahui pengaruh penambahan MnSO4 terhadap aktivitas enzim MnP Phlebia sp. MG-60 dalam proses delignifikasi selama 28 hari inkubasi. Mangan dengan konsentrasi 0 mM; 0,1 mM; dan 0.5 mM ditambahkan pada substrat yang telah diinokulasi dengan Phlebia sp. MG-60 dan diinkubasi selama 28 hari. Hasil kemudian diekstrak selanjutnya diuji kadar lignin dan aktivitas enzim MnP yang dihasilkan menggunakan spektofotometri. Hasil menunjukkan aktivitas enzim MnP bagasse yang paling besar dengan penambahan 0,1 mM MnSO4 0.605 IU/mL dengan lignin removal mencapai 31.60%.

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Solid State production of manganese peroxidases using arecanut husk as substrate

Abstract: ABSTRACT

Cited by 20 publications

References 14 publications

Archives of Environmental Protection

Archives of Environmental Protection

Cultivation of Mushrooms and Their Lignocellulolytic Enzyme Production Through the Utilization of Agro-Industrial Waste

PENGARUH PENAMBAHAN MnSO4 TERHADAP AKTIVITAS ENZIM MANGAN PEROKSIDASE PADA DELIGNIFIKASI LIMBAH BAGASSE OLEH PHLEBIA SP. MG-60

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