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Cited by 11 publications
(2 citation statements)
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“…Traditional studies on bacterial diversity in foods have relied on cultivation methods, whereas only about 1% of microorganisms in the natural environment can be cultivated through pure culture methods [ 9 ]. Culture-independent technologies that involve molecular biology approaches, such as gradient gel electrophoresis (DGGE) and polymerase chain reaction (PCR), have been applied to the evaluation of microbial diversity in a variety of environments, including foods, avoiding the limitations of traditional microbial analyses [ 10 , 11 , 12 , 13 ]. These measurement methods have been expanded using high-throughput sequencing (HTS) to analyze microbial community structures [ 9 ].…”
Section: Introductionmentioning
confidence: 99%
“…Traditional studies on bacterial diversity in foods have relied on cultivation methods, whereas only about 1% of microorganisms in the natural environment can be cultivated through pure culture methods [ 9 ]. Culture-independent technologies that involve molecular biology approaches, such as gradient gel electrophoresis (DGGE) and polymerase chain reaction (PCR), have been applied to the evaluation of microbial diversity in a variety of environments, including foods, avoiding the limitations of traditional microbial analyses [ 10 , 11 , 12 , 13 ]. These measurement methods have been expanded using high-throughput sequencing (HTS) to analyze microbial community structures [ 9 ].…”
Section: Introductionmentioning
confidence: 99%
“…Fungi identification and classification lie on the traditional analyses of macroscopic and microscopic structures (Larone 2002, Watanabe 2002, and their characteristics may change considerably depending on the environment and the conditions they are exposed to. Therefore, molecular methods are tools that help to differentiate the microorganisms (Jimenez et al 1999, Mirhosseini et al 2011. Ribosomal DNA (rDNA) is an interesting target to fungal phylogeny studies as it encodes the highly polymorphic 600-800 bp ITS region (Internal Transcribed Spacers), being used for the differentiation of many yeast and fungal species (Larena et al 1999, Anderson and Cairney 2004, Pinheiro 2004, Anderson and Parkin 2007.…”
Section: Introductionmentioning
confidence: 99%