Leaf surface characters applied to Lauraceae taxonomy in a Seasonal Forest of Southern Brazil

Abstract

The goal of this work was to test if the macro and micro morphological analysis of the leaf surface could provide vegetative diagnostic characters for some of the most common Lauraceae species in Southern Brazilian Seasonal Forests. The leaf printing technique with universal instantaneous adhesive was used for the epidermical microscopical analysis and external macroscospical analyses of leave were made. Microscopic evaluation revealed the visibility and contours of anticlinal walls of epidermical cell and stomata and shape of guard-cells. Macroscopic evaluation showed the absence or presence of characters such as hairiness, domatia and scents.The results showed that analyzed characters, together with other diagnostic characteristics, could contribute in taxonomic delimitation of some common Lauraceae species in the Southern Brazil.

Use of Frozen Leaves for Morpho-anatomical Characterization of Nectandra megapotamica (Spreng.) Mez., Lauraceae

“…2). Similar pattern was reported by another research with the same species [11], in which the epidermal cells exhibit straight anticlinal walls, stomata and guard cells almost imperceptible (100x). The cuticle and the cutinized portion of epidermal cells are thin, and the cutinization of the epidermal cells occurs only in the external periclinal walls.…”

Microscopic investigations of Ocotea paranaensis―A Brazilian endemic and endangered species

“…Simple non‐glandular trichomes were found in O. paranaensis , as also observed in some other Ocotea species (Brustulim et al, 2020; Ceolin et al, 2009; Coutinho, Agra, Barbosa‐Filho, & Basilio, 2006; Farago et al, 2005; Gonçalves et al, 2018). However, these trichomes were not observed in O. nutans (Nees) Mez (Betim et al, 2020).…”

“…Paracytic type and hypostomatic leaves are frequently observed in Ocotea species (Betim et al, 2020;Brustulim et al, 2020;Farago et al, 2005;Gomes-Bezerra et al, 2018;Gonçalves et al, 2018). Trofimov and Rohwer (2018) Simple non-glandular trichomes were found in O. paranaensis, as also observed in some other Ocotea species (Brustulim et al, 2020;Ceolin et al, 2009;Farago et al, 2005;Gonçalves et al, 2018). However, these trichomes were not observed in O. nutans (Nees) Mez (Betim et al, 2020).…”

La arquitectura foliar de las especies de Lauraceae Nativas de Misiones, Argentina.

“…Las características de la epidermis foliar observadas en N. megapotamica, N. lanceolata, O. puberula y O. pulchella son coincidentes con lo descripto por Ceolin et al (2009). Esta característica, puede ser un carácter sumamente importante para poder separar en grupos las especies en estudios más detallados para la identificación de las mismas, en el caso en que los caracteres macroscópicos no permitan separar las especies.…”