A study of ion-exchange chromatography in an expanded bed for bovine albumin recovery

Severo, João Baptista; Souza, Roberto Rodrigues de; Santana, José Carlos Curvelo; Tambourgi, Elias Basile

doi:10.1590/s1516-89132009000200021

Cited by 4 publications

(2 citation statements)

References 14 publications

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“…Monobasic potassium phosphate (24.36, 12.72 and 2.2g) and dibasic potassium phosphate (1.18, 6.2 and 10.68g) were dissolved in 1000 mL of distilled water, respectively for pH 5.7, 6.7 and 7.7. Its pH was adjusted with phosphoric acid or potassium hydroxide (Morita and Assumpção 1995;Ferreira et al 2011;Ferreira et al 2007;Severo Júnior et al 2007;Padilha et al 2012;Severo Jr et al 2009).…”

Section: Preparation Of Buffer Solutionsmentioning

confidence: 99%

Determination of optimal condition to obtain the bromelain from pineapple plants produced by micropropagation

Leite

Lima

Santana

et al. 2012

Braz. arch. biol. technol.

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This study aimed to obtain the condition of maximum bromalein activity in different parts of pineapple plants produced in vitro, by micropropagation. The sStems and leaves of Pérola and Imperial cultivar plants were evaluated after three and eight months of in vitro cultivation in Murashige and Skoog medium without growth phytoregulator, macerated in potassium phosphate buffer at different pH values (5.7, 6.7 and 7.7). Total protein and proteolytic activity were determined in the samples after three- and eight-month cultivation periods. For both the cultivars, the best results were obtained at pH 5.7 in extraction media. Pérola cultivar, showed higher bromelain activity in the leaves cultivated in vitro for three months (0.0194U/mL) while in the Imperial cultivar, it was higher in the stem after eight months (0.0179 U/mL). Imperial cultivar showed higher bromelain activity than the Pérola's

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Section: Preparation Of Buffer Solutionsmentioning

confidence: 99%

Determination of optimal condition to obtain the bromelain from pineapple plants produced by micropropagation

Leite

Lima

Santana

et al. 2012

Braz. arch. biol. technol.

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“…This technology combines solid–liquid separation, concentration and primary purification into one unit, which can certainly reduce the processing times and improve the process efficiency. − In addition, EBA has high productivity due to relatively high operation velocity and low back-pressure. During the past two decades, many kinds of EBA processes have been developed for primary protein separation, such as ion exchange, , hydrophobic interaction, − affinity, − etc. In recent years, mixed-mode chromatography (MMC) was introduced as a novel technology for protein separation, which combines multiple binding modes, like ionic exchange, hydrophobic interaction, hydrogen bonding, etc.…”

Section: Introductionmentioning

confidence: 99%

Integration of Expanded Bed Adsorption and Hydrophobic Charge-Induction Chromatography for Monoclonal Antibody Separation

Shi

Dong

Yao

et al. 2017

Ind. Eng. Chem. Res.

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The integration of expanded bed adsorption (EBA) with hydrophobic charge-induction chromatography (HCIC) affords a promising new technology to capture antibody from the complex feedstock. New EBA resin T-ABI was prepared with hydrophobic charge-induction ligand 5-aminobenzimidazole (ABI) and used to separate monoclonal antibody (mAb) from CHO cell culture broth. The static and dynamic adsorption behaviors of hIgG and mAb were investigated, and the typical properties of pH dependence and salt tolerance were found. High dynamic binding capacities at 10% breakthrough (18.1–21.4 mg/mL resin) were obtained even for high operation velocities (711–1203 cm/h) in an expanded bed. By optimization of loading pH, elution pH, and expansion factor, hIgG could be separated from the protein mixture (2 mg/mL hIgG and 10 mg/mL bovine serum albumin) with high efficiency. Finally, mAb was separated directly from the CHO cell culture broth with T-ABI EBA under optimized conditions (loading at pH 7.0, elution at pH 4.0 or 4.5, expansion factor of 2.0), and the purity reached 93.7–97.7% with the recovery of 72.6–79.4%. The results indicated that T-ABI resin was suitable to capture hIgG from the complicated feedstock. New separation process with T-ABI EBA showed a promising potential for antibody purification with high productivity and process efficiency, which combined the advantages of HCIC and EBA.

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Modelling water quality and quantity with the influence of inter-basin water diversion projects and cascade reservoirs in the Middle-lower Hanjiang River

Wang

Zhang

Zhao

et al. 2016

Journal of Hydrology

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A study of ion-exchange chromatography in an expanded bed for bovine albumin recovery

Abstract: ABSTRACT

Cited by 4 publications

References 14 publications

Determination of optimal condition to obtain the bromelain from pineapple plants produced by micropropagation

Determination of optimal condition to obtain the bromelain from pineapple plants produced by micropropagation

Integration of Expanded Bed Adsorption and Hydrophobic Charge-Induction Chromatography for Monoclonal Antibody Separation

Modelling water quality and quantity with the influence of inter-basin water diversion projects and cascade reservoirs in the Middle-lower Hanjiang River

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