Detection of GBV-C/HGV RNA in cervico-vaginal smears from healthy individuals

Watanabe, Maria Angélica Ehara; Miquelão, Ana Karina M. Benthien; Oliveira, Carlos Eduardo Coral de; Oliveira, Karen Brajão de; Nasser, Thiago Franco; Aoki, Mateus Nóbrega; Suzuki, Patrícia Sayuri; Amarante, Marla Karine; Venâncio, Emerson José; Azevedo, Elbens Marcos Minoreli de

doi:10.1590/s1516-89132008000500007

Cited by 2 publications

(2 citation statements)

References 30 publications

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“…A study performed in Brazil supports the importance of condoms for preventing sexual transmission of HPgV . Cervicovaginal smears were collected from 73 healthy women who had undergone routine screening for cervical cancer; a high prevalence of HPgV (17.6%) was found in the genital mucosa of this population .…”

Section: Discussionmentioning

confidence: 87%

“…24 Cervicovaginal smears were collected from 73 healthy women who had undergone routine screening for cervical cancer; a high prevalence of HPgV (17.6%) was found in the genital mucosa of this population. 24 Serum extravasation could not be ruled out by the researchers as no blood samples were tested; however, the high prevalence reported suggested that the genital mucosa was the first line of defense for sexually transmitted infections.…”

Section: Discussionmentioning

confidence: 98%

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Prevalence and vertical transmission of human pegivirus among pregnant women infected with HIV

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Lobato

Barral

et al. 2017

Intl J Gynecology & Obste

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Section: Discussionmentioning

confidence: 87%

Section: Discussionmentioning

confidence: 98%

Prevalence and vertical transmission of human pegivirus among pregnant women infected with HIV

Santos

Lobato

Barral

et al. 2017

Intl J Gynecology & Obste

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Current molecular methods for the detection of GB virus C

Valinčiūtė-Jankauskienė

Mauricas

2017

Reviews in Medical Microbiology

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The GB virus C (GBV-C), also referred to as hepatitis G virus, is a RNA hepatitis virus, which infects humans naturally and can cause high levels of viremia, which, however, in most cases, does not lead to serious illness. It is not usually routinely screened in clinical laboratories, though its presence in patients infected with other viruses such as hepatitis C virus and HIV has been commonly reported. The virus can be detected in the blood of infected individuals using reverse-transcription polymerase chain reaction (RT-PCR) mainly. It is a technique that is commonly used for different RNA material detection and other screening such as gene expression and testing. The method includes several steps, that is, transcription of the RNA into complementary DNA (cDNA) by reverse transcriptase, amplification of the cDNA by PCR using well defined primers that target a particular region of the gene, and detection of the PCR products by various means such as agarose gel electrophoresis or hybridization and immunoassay using, for example, automated detection systems. Several primers targeting different regions of the genome, that is, NS3, NS5, and 5’ NTR, have been developed for the amplification of the specific region and the detection of the virus. Moreover, variants of the RT-PCR have been designed to maximize the identification potential. These include, for example, RT-PCR-ELISA, RT digital-PCR, and SYBR green real-time multiplex RT-PCR. Another type of method used to screen the virus is the detection of the presence of antibodies E2 (anti-E2) produced against the viral glycoprotein E2 in individuals who have been infected with the virus and have recovered or are in the process of recovering. The anti-E2s are detected by radio-immunoprecipitation assay and ELISA. Use of both antibody and RNA detection methods is necessary for a better epidemiological investigation of the prevalence of GBV-C.

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Detection of GBV-C/HGV RNA in cervico-vaginal smears from healthy individuals

Cited by 2 publications

References 30 publications

Prevalence and vertical transmission of human pegivirus among pregnant women infected with HIV

Prevalence and vertical transmission of human pegivirus among pregnant women infected with HIV

Current molecular methods for the detection of GB virus C

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