Characterization of Colletotrichum lindemuthianum isolates using differential cultivars of common bean in Santa Catarina State, Brazil

Abstract

In 2003 and 2004, 32 isolates of Colletotrichum lindemuthianum obtained from the infected plants of field-grown common bean (Phaseolus vulgaris L.) in Santa Catarina state, Brazil were analyzed based on the virulence to 12 differential cultivars of Phaseolus vulgaris L.. Thirteen distinct races were identified, six of which had not been reported previously in Santa Catarina. This is the first report of the occurrence of 67, 83,101,103,105, and 581 races of C. lindemuthianum. Race 65 was most common (34%). All the isolates were compatible to the cultivars Michelite and Mexico 222. Some isolates infected not only differential cultivar of Mesoamerican origin, but also the ones of Andean origin.<br>Em 2003 e 2004, isolados de Colletotrichum lindemuthianum obtidas de plantas infectadas de feijoeiro comum (Phaseolus vulgaris L.) no Estado de Santa Catarina, Brasil foram analisadas baseando-se na virulência em 12 cultivares diferenciadoras de Phaseolus vulgaris L.. Treze raças distintas foram identificadas, sendo que seis delas não haviam sido reportadas anteriormente em Santa Catarina. Este é o primeiro registro de ocorrência das raças 67, 83, 101,103,105, e 581 do C. lindemuthianum. A raça mais comum foi a 65 (34%). Todos os isolados foram compatíveis com as cultivares Michelite e México 222. Alguns dos isolados infectaram tanto cultivares diferenciadoras de origem Mesoamericana como de origem Andina

Colletotrichum isolates related to Anthracnose of cashew trees in Brazil: morphological and molecular description using LSU rDNA sequences

“…In general, such symptoms are similar in all cashew hosts from different localities, but the isolates of the pathogen may vary in its degree of aggressiveness as well as in its cultural and morphological characters. Species concepts based on morphological criteria are generally broad for the genus Colletotrichum, and wide variations for cultural and morphological characters, pathogenicity and host range have been reported among isolates of C. gloeosporioides (Alahakoon and Brown, 1994;Gonçalves-Vidigal et al 2008;Jeffries et al, 1990;Skipp et al, 1995;Menezes and Hanlin, 1996a, b;Sera et al 2007). Sequence analyses of the intergenic transcribed spacers (ITS 1, ITS 2) and Domains (D1,D2) of large subunit (LSU) ribosomal DNA (rDNA) have been valuable for delineating isolates and species from this genus (Adaskaveg and Hartin, 1997;Forseille, 2007;Freeman et al, 2001;Johnston, 2000;Johnston and Jones, 1997;Moriwaki et al, 2002;Sherriff et al, 1994;Vinnere et al, 2002).…”

<b>Characterization of race 65 of <i>Colletotrichum lindemuthianum</i> by sequencing ITS regions

“…In turn, Group II consists of twelve isolates of race 65, the isolates of race 17, race 2, MAFF 305390, R23.19, Rec15, the subgroup with isolate 11 from the State of Santa Catarina belonging to race 65, and races 23, 31, 73 and 1096. Gonçalves-Vidigal and Kelly (2006) used an isolate of race 65 that overcame resistance in cultivar BAT93 that differed from the one used by Alzate-Marin et al (2007) for which BAT93 showed resistance. In the present study, two groups within race 65 were observed in the phylogenetic tree.…”

“…Race 65 has been reported as one of the most frequent and widely distributed races in several countries, including Brazil, the United States, Mexico, Argentina, Equator, Africa and India, with increased prominence in the past 30 years anos (Pastor-Corrales, Erazo, Estrada, & Singh, 1994;Rava et al, 1994;Balardin, Jarosz, & Kelly, 1997;Balardin & Kelly, 1998;Sharma, Kumar, Sharma, Sud, & Tyagi, 1999;Thomazella et al, 2002;AlzateMarin & Sartorato, 2004;Mahuku & Riascos, 2004;Talamini et al, 2004;Bonett, Schewe, & Silva, 2008;Gonçalves-Vidigal, Thomazella, Vidigal Filho, Kvitschal, & Elias, 2008;Gonçalves-Vidigal et al, 2009). Consequently, breeding programs have focused their attention on this race with the goal of controlling anthracnose (Davide & Souza, 2009).…”

Transfer of Co-1 Gene Locus for Anthracnose Disease Resistance to Fresh Bean (Phaseolus vulgaris L.) Through Hybridization and Molecular Marker-Assisted Selection (MAS)

“…Dwarf fresh bean lines T23, TK57, TK1, Ç31, Ç28, T7, T26, TK15 and T21 with Ayşe Kadın properties and anthracnose resistant MDRK, PM, Kaboon, Widusa, Machinac, Isles, Chinook varieties were inoculated with strains, 2175, 4071, 3303, 3321 and 3071 (Madakbaş et al, 2006). Evaluations were performed on the scale of 0 to 9 (0-3 resistant; 4-9 sensitive) (Goncalves-Vidigal, Thomazella, Elias, & Vidigal-Filho, 2004b). …”