Production and characterization of galactose oxidase produced by four isolates of Fusarium graminearum

Abstract

A screening aimed to find new galactose oxidase producer isolates and to evaluate the production among Fusarium graminearum strains was conducted. Thirty-five isolates out of 39 analysed produced the enzyme at several levels. The data indicated a wide distribution of galactose oxidase within F. graminearum and also revealed new producer isolates. The enzyme produced by different isolates showed similar thermal activity and stability and were active on same substrates. However, the optimum pH ranged from 7.0 to 7.5. Thus, all evaluated isolates were suitable for the production of galactose oxidase.

Expression, purification, and characterization of galactose oxidase of Fusarium sambucinum in E. coli

“…Below pH 5 GalOx shows no activity. This is in good agreement with data reported previously for native GalOx from different sources [44,48,49] .…”

“…The enzyme is secreted by a number of fungal species, of which Fusarium graminearum (formerly classified as Dactylium dendroides ) is the most extensively studied [35–43] . The production and purification of GalOx has been reported from its natural fungal source [26,39,44–49] , furthermore, various GalOx genes were cloned and successfully expressed in the filamentous fungi Aspergillus nidulans [50,51] , Aspergillus oryzae and Fusarium venenatum [52] , which have no endogenous GalOx, in the methylotrophic yeast Pichia pastoris [4,10,33,36,53–56] and in the bacterium Escherichia coli [55,57–59] . Typically, wild-type fungal GalOx is produced as a preproform carrying an N-terminal signal sequence, which is removed upon secretion, yielding the immature proform.…”

Galactose Oxidase from Fusarium oxysporum - Expression in E. coli and P. pastoris and Biochemical Characterization

“…At pH values below 5.0 the enzyme lost its activity. The optimum pH of recombinant GalOx from F. oxysporum described here is in good agreement with data reported previously for the native enzyme purified from other sources [5] , [7] , [41] .…”

Oxidation with galactose oxidase: Multifunctional enzymatic catalysis

“…subglutinans, and F. acuminatum [18]. Gasparetto et al subsequently studied 39 Fusarium graminearum strains and found that 35 secreted GAO where four strains were considered particularly good producers [19]. Cordeiro subglutinans [20].…”