In Vitro study of Vellozia pusilla pohl (Velloziaceae), a Brazilian plant species: antitumoral activity and labeling of blood elements

Dantas, Ana Letícia Almeida; Valente, Lígia M. M.; Morais, L. A. S. de; Feliciano, Gláucio Diré; Bernardo-Filho, Mário

doi:10.1590/s1516-89132005000700008

Cited by 4 publications

(3 citation statements)

References 7 publications

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“…The technetium has been the most utilized radionuclide in the nuclear medicine because of its acceptable physical characteristics: six hours of plasma half-life and despicable environment impact (Saha, 1998). It is used in the study of the natural or synthetic drugs influence in the labeling of the RBC and the plasma proteins Dantas et al, 2005;Holanda, 2004;Oliveira et al, 2000;Souza et al, 2005). The aim of this study was to evaluate the capability of different concentrations of the non-oily fraction of R. communis to modify the physiological properties (osmotic fragility), labeling of the RBC and plasma proteins with the technetium-99m and morphology of the cells.…”

Section: Introductionmentioning

confidence: 99%

Effect of the extract of Ricinus communis L. on the osmotic fragility, labeling of red blood cells with Technetium-99m and morphology of the cells

Mousinho

Correia

Silva

et al. 2008

Braz. arch. biol. technol.

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The aim of this study was to evaluate the influence of the proteic extract of R. communis on the cell physiology by the osmotic fragility, labeling of the blood elements with the 99mTc and cell morphology. To evaluate the osmotic fragility, the blood samples of the Wistar rats were incubated with the concentrations of R. communis and with the solutions of NaCl (0.4; 0.7; 0.9%). In the labeling of the blood elements procedure, the rat blood was treated with a solution of determining the rate of radioactivity (%ATI) in the plasma (P) and in the red blood cells (RBC). The soluble and insoluble fractions of the plasma were also evaluated. The cells morphology submitted to the extract was evaluated by the optical microscopy (x40

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Section: Introductionmentioning

confidence: 99%

Effect of the extract of Ricinus communis L. on the osmotic fragility, labeling of red blood cells with Technetium-99m and morphology of the cells

Mousinho

Correia

Silva

et al. 2008

Braz. arch. biol. technol.

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“…O Tc-99m possui características físicas aceitáveis, como meia-vida de 6 horas, emissão de fóton gama de 140 keV, impacto ambiental desprezível e baixa exposição da radiação para o paciente (Govindan et al, 1996;Dantas et al, 2005;Oliveira et al, 2000;Souza et al, 2005;Valença et al, 2005). Entretanto, vários fatores podem interferir na marcação e biodistribuição dos radionuclídio, e dentre eles estão os produtos naturais (Braga et al, 2000;Bernardo-Filho et al, 2005;Dantas et al, 2005).…”

Section: Introductionunclassified

Efeito da fração não-oleosa doRicinus communisna biodistribuição comTecnécio-99m em camundongos

Mousinho

Rocha

Pereira

et al. 2015

Rev. bras. plantas med.

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RESUMO:Ricinus communis é uma planta pertence à família Euforbiaceae. A partir de seus frutos é extraído o óleo de rícino e outra fração altamente tóxica (fração não-oleosa). Neste estudo, foi avaliado o efeito da fração não oleosa de R. communis (FNORC) na biodistribuição do Tc-99m em camundongos sadios e em animais transplantados com Sarcoma-180 (S180). Para avaliação da biodistribuição do Tc-99m, o percentual de atividade radioativa total injetada (ATI%) foi mensurada utilizando um contador gama. Os resultados mostraram que, em animais sadios, houve aumento da captação do Tc-99m após o tratamento com FNORC pelos rins, cérebro e estômago. Apenas houve diminuição da absorção deste radioisótopo nos músculos. Em animais com tumor, houve redução significativa da captação do Tc-99m no coração, pulmões e tumor, e foi observado o aumento da captação apenas nos rins. Foi observada também a diminuição da concentração das proteínas plasmáticas totais em animais com tumor após tratamento com FNORC e atividade antitumoral significativa. Diante desses dados, conclui-se que fração não oleosa de R. communis possui atividade antitumoral significativa, porém altera a biodistribuição do Tc-99m, fato este que pode resultar em falhas nos processos de diagnóstico por imagem que utilizem este radioisótopo como marcador. Palavras-chave:Ricinus communis, Tecnécio-99m, Biodistribuição, Proteínas totais, Atividade antitumoral. ABSTRACT: Effect of non-oily fraction of Ricinus communis on the bio distribution withTechnetium-99m in mice. Ricinus communis is a plant belonging to the Euforbiaceae family. From its fruits, it is extracted the castor oil and another highly toxic fraction (non-oily fraction). In this study, we evaluated the effect of the non-oily fraction of R. communis (NOFRC) on the bio distribution of Tc-99m in healthy mice and in animals transplanted with Sarcoma-180 (S180). To evaluate the bio distribution of theTc-99m, the percentage of the total injected radioactivity (% TIR) was measured through a gamma counter. There was an increase in the capitation of Tc-99m after the treatment with NOFRC in the kidneys, brain, and stomach of healthy animals. A decrease in the reception of this radionuclide was only found in the muscles. In animals with tumour, there was a significant reduction in the uptake of Tc-99m in the heart, lungs and tumour. An increase in the capitation was only observed in the kidneys. It was also observed a reduction in the total plasma protein concentration in animals with tumours after the treatment with NOFRC, besides significant antitumor activity. We concluded that there is antitumor activity in the NOFRC, but that it alters the biodistribution of Tc-99m, a fact which may result in failures in the diagnostic imaging process using this radioisotope as a marker.

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“…There are some studies about the effect of natural products on the labeling of blood constituents using technetium-99m (Tc-99m) (Lima et al, 2002;Oliveira et al, 1997;Braga et al, 2000;Oliveira et al, 2003;Dantas et al, 2005;Oliveira-Fernandes et al, 2005;Abreu et al, 2006;Neves et al, 2007;Rebello et al, 2007;Sinzinger et al, 2007). It has been reported that Fucus vesiculosus (Oliveira et al, 2003), Ginkgo biloba (Moreno et al, 2004), Psidium guajava (Abreu et al, 2006), Vellozia pusilla (Dantas et al, 2005) and Arctium lappa extracts are capable of altering the radiolabeling of blood constituents. The alteration of fixation of Tc-99m on the blood constituents promoted by some drugs has been associated with a possible oxidation of the stannous ion .…”

Section: Introductionmentioning

confidence: 99%

Uncaria tomentosa extract: evaluation of effects on the in vitro and in vivo labeling of blood constituents with technetium-99m

Moreno

Carvalho

Nascimento

et al. 2008

Braz. arch. biol. technol.

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In Vitro study of Vellozia pusilla pohl (Velloziaceae), a Brazilian plant species: antitumoral activity and labeling of blood elements

Cited by 4 publications

References 7 publications

Effect of the extract of Ricinus communis L. on the osmotic fragility, labeling of red blood cells with Technetium-99m and morphology of the cells

Effect of the extract of Ricinus communis L. on the osmotic fragility, labeling of red blood cells with Technetium-99m and morphology of the cells

Efeito da fração não-oleosa doRicinus communisna biodistribuição comTecnécio-99m em camundongos

Uncaria tomentosa extract: evaluation of effects on the in vitro and in vivo labeling of blood constituents with technetium-99m

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