Comparisons between continuous and batch processing to produce clavulanic acid by Streptomyces clavuligerus

Neto, Álvaro de Baptista; Teodoro, Juliana Conceição; Filho, L. C. M. Cassiano; Badino, Alberto Colli; Hokka, Carlos O.

doi:10.1590/s1516-89132005000400012

Cited by 10 publications

(6 citation statements)

References 5 publications

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“…There is a growing body of literature related to the comparison between airlift and conventional bioreactors for different applications [12][13][14][15][16][17]. Regarding clavulanic acid production, various studies can be found in the literature related to the improvements in the composition of culture media [6,8,[18][19][20], bioreactor operation mode [3,5,7,[21][22][23] and the influence of oxygen supply and agitation condition on CA production by S. clavuligerus in conventional bench scale bioreactor [4]. However, to the best of our knowledge no articles which compare the performances of conventional and non-conventional pneumatic bioreactors on CA production can be found.…”

Section: Introductionmentioning

confidence: 99%

Shear conditions in clavulanic acid production by Streptomyces clavuligerus in stirred tank and airlift bioreactors

Cerri

Badino

2012

Bioprocess Biosyst Eng

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In biochemical processes involving filamentous microorganisms, the high shear rate may damage suspended cells leading to viability loss and cell disruption. In this work, the influence of the shear conditions in clavulanic acid (CA) production by Streptomyces clavuligerus was evaluated in a 4-dm(3) conventional stirred tank (STB) and in 6-dm(3) concentric-tube airlift (ALB) bioreactors. Batch cultivations were performed in a STB at 600 and 800 rpm and 0.5 vvm (cultivations B1 and B2) and in ALB at 3.0 and 4.1 vvm (cultivations A1 and A2) to define two initial oxygen transfer conditions in both bioreactors. The average shear rate ([Formula: see text]) of the cultivations was estimated using correlations of recent literature based on experimental data of rheological properties of the broth (consistency index, K, and flow index, n) and operating conditions, impeller speed (N) for STB and superficial gas velocity in the riser (UGR) for ALB. In the same oxygen transfer condition, the [Formula: see text] values for ALB were higher than those obtained in STB. The maximum [Formula: see text] presented a strong correlation with a maximum consistency index (K (max)) of the broth. Close values of maximum CA production were obtained in cultivations A1 and A2 (454 and 442 mg L(-1)) with similar maximum [Formula: see text] values of 4,247 and 4,225 s(-1). In cultivations B1 and B2, the maximum CA production of 269 and 402 mg L(-1) were reached with a maximum [Formula: see text] of 904 and 1,786 s(-1). The results show that high values of average shear rate increase the CA production regardless of the oxygen transfer condition and bioreactor model.

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Section: Introductionmentioning

confidence: 99%

Shear conditions in clavulanic acid production by Streptomyces clavuligerus in stirred tank and airlift bioreactors

Cerri

Badino

2012

Bioprocess Biosyst Eng

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“…One of the advantages of this method is the high recovery yield by this process. Also, as reported by Baptista Neto et al [6] in a study of clavulanic acid production, microfiltration can be used to separate the microorganism in continuous cultures with cell recycling.…”

Section: Introductionmentioning

confidence: 89%

Preliminary Studies for Cephamycin C Purification Technique

Neto

Bustamante

Luiz

et al. 2011

Appl Biochem Biotechnol

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A study was made for purification of cephamycin C from fermentation of Streptomyces clavuligerus. Initially, the culture broth was clarified by microfiltration and ultrafiltration, after which the resulting permeates were subjected to nonspecific adsorption and ion-exchange chromatography on resin columns. The antibiotic activity was measured by the biological method at each stage by assaying its activity against the Escherichia coli ESS, super sensitive to β-lactam antibiotic. The purification processes were assessed in relation to the variables affecting each step. The purification efficiency by nonspecific adsorption was monitored by UV spectrophotometry, while the ion-exchange adsorption fractions were assessed by NMR spectroscopy. Some of the fractions obtained during purification were also analyzed by mass spectrometry (LC/MS and LC/MS/MS) to identify the cephamycin C molecule. These preliminary results proved the process feasibility.

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“…Carbono Glicerol (aceite de palma y oliva), glucosa, almidón. [4,[8][9][10][11][12] Nitrógeno Ornitina, prolina, ácido glutámico, proteína aislada de soya, harina de soya, peptona, sulfato de amonio, glutamato monosódico, asparagina. [8,11,13] Oxígeno Oxígeno.…”

Section: Fuente Nutrientes Referenciasunclassified

“…[4,[8][9][10][11][12] Nitrógeno Ornitina, prolina, ácido glutámico, proteína aislada de soya, harina de soya, peptona, sulfato de amonio, glutamato monosódico, asparagina. [8,11,13] Oxígeno Oxígeno. [5,7,13] Elementos traza Cloruro de sodio, sulfato de magnesio, sulfato de manganeso, cloruro de manganeso, carbonato de calcio, sulfato de cobre, cloruro de zinc.…”

Section: Fuente Nutrientes Referenciasunclassified

“…[5,7,13] Elementos traza Cloruro de sodio, sulfato de magnesio, sulfato de manganeso, cloruro de manganeso, carbonato de calcio, sulfato de cobre, cloruro de zinc. [4,12,13] Soluciones buffer y antiespumantes Siliconado (Antifoam A), solución buffer (MOPS) [7][8][9] Reguladores de pH Hidróxido de sodio, ácido clorhídrico y ácido sulfúrico. [7,8] La diferente combinación o uso de nutrientes (en cantidad y/o naturaleza), sumado a las diversas condiciones de operación, dan lugar a diferentes rendimientos hacia AC que dificultan la comparación objetiva de los procesos y, por lo tanto, definir a partir de la literatura unas "mejores condiciones" para llevarlo a cabo.…”

Section: Fuente Nutrientes Referenciasunclassified

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Simulación del proceso de producción de ácido clavulánico

Betancur¹,

Arias²,

Zapata³

2023

Entre cienc. ing.

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El Ácido Clavulánico es un metabolito ampliamente utilizado con distintas combinaciones de antibióticos para contrarrestar diferentes enfermedades infecciosas. Este metabolito no se produce en Colombia a pesar de tener una gran demanda a nivel nacional, aparentemente debido a sus altos costos de producción y a la poca información que se tiene del proceso global. En esta investigación se usan diferentes reportes de la literatura para presentar un esquema generalizado de la producción de este metabolito y, con ayuda del software Aspen plus, la implementación de un modelo para simular el proceso de producción, incluyendo etapas de pretratamiento, producción y postratamiento o purificación. El modelo resulta de gran utilidad para analizar costos o evaluar eventuales cambios en el proceso de producción, constituyendo una herramienta útil para el estudio y entendimiento del proceso, así como para la toma de decisiones frente a su eventual implementación.

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Comparisons between continuous and batch processing to produce clavulanic acid by Streptomyces clavuligerus

Abstract: ABSTRACT

Cited by 10 publications

References 5 publications

Shear conditions in clavulanic acid production by Streptomyces clavuligerus in stirred tank and airlift bioreactors

Shear conditions in clavulanic acid production by Streptomyces clavuligerus in stirred tank and airlift bioreactors

Preliminary Studies for Cephamycin C Purification Technique

Simulación del proceso de producción de ácido clavulánico

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