Induction of cassava somatic embryogenesis in liquid medium associated to floating membrane rafts

Takahashi, Elizabete Keiko; Kobayashi, Adilson Kenji; Vieira, Luiz Gonzaga Esteves

doi:10.1590/s1516-89132000000100005

Cited by 2 publications

(2 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Modernas metodologias da engenharia genética estão sendo utilizadas pelos pesquisadores para produzir plantas resistentes a pragas, enfermidades, herbicidas e estresses abióticos (PUONTI KAERLAS et al, 1999;RAEMAKERS et al, 1996;VAEZ, 2007;ZHANG et al, 2005). Para que novas cultivares possam ser obtidas em laboratório, um dos pré-requisitos é que existam sistemas in vitro que possibilitem a regeneração das plantas mediante a organogênese e embriogênese somática (MATSUMOTO et al, 1991;FALOCI;MROGINSKI, 2001;TAKAHASHI;KOBAYASHI;VIEIRA, 2000;VIDAL, 2009).…”

Section: O Melhoramento De Mandiocaunclassified

“…Em mandioca, embriões somáticos têm sido induzidos a partir de cotilédones, ápices caulinares e folhas jovens clonadas in vitro (FEITOSA et al, 2007;MATSUMOTO et al, 1991;FALOCI;MROGINSKI, 2001;STAMP;HENSHAW 1987;SZABADOS et al, 1987;TAKAHASHI;KOBAYASHI;VIEIRA, 2000;VIDAL, 2009). Entretanto, os protocolos de embriogênese somática em mandioca ainda não estão bem estabelecidos.…”

Section: Cultura De Tecidos Vegetaisunclassified

See 1 more Smart Citation

Indução de poliploidia em mandioca

Silva¹

View full text Add to dashboard Cite

The induction of polyploidy in cassava The polyploidy played an important role in the improvement of many species of plants, especially in cereals including intergeneric hybrids. The process of polyploidy usually produces new and desirable phenotypes or provides support for crosses of plants in breeding programs. This research work reports the result of polyploidy induction in two cassava cultivars ('Porquinho' and 'Vassourinha') by different colchicine concentrations (0.05 %, 0.10 % and 0.15 %) in culture medium exposed for 48 or 96 hours. Regenerated plants were examined cytologically by flow cytometry and chromosome counting. Additionally, analysis of the size and frequency of stomata were performed and the results showed that can be used for screening of the treated plants. Treatment with 0.1% colchicine in the culture medium for 96 hours showed a higher number of tetraploid plants produced about the number of regenerated plants in both cultivars evaluated. No significant differences among colchicine doses and duration times treatment were found in the number of tetraploids found in each treatment. However, we observed a significant difference in tetraploid production between the cultivars analyzed, indicating that there may be a genetic response to induction of chromosome doubling with colchicine.

show abstract

Section: O Melhoramento De Mandiocaunclassified

Section: Cultura De Tecidos Vegetaisunclassified

Indução de poliploidia em mandioca

Silva¹

View full text Add to dashboard Cite

show abstract

Somatic embryogenesis in two Nigerian cassava cultivars (Sandpaper and TMS 60444)

Ubalua¹,

Mbanaso²

2014

J. Evol. Biol. Res.

View full text Add to dashboard Cite

The embryogenic ability of two cassava cultivars (Sandpaper and TMS 60444) was investigated by culturing their young leaf lobes on an induction medium (DKW2 50P). The explants formed organized embryogenic structures regardless of the concentration of picloram in the medium. Organized embryogenic structures induction was enhanced by increasing the concentration of picloram in the induction medium for both cultivars. The optimum level of picloram at which maximum stable frequencies (53.1±17.6 and 51.5±14.6 for Sandpaper and TMS 60444 respectively) of organized embryogenic structures were obtained was 5 mg/l. Friable embryogenic callus were further produced in GD2 50P medium supplemented with 500 µM tyrosine. The produced friable embryogenic calluses are prime target tissues for genetic transformation and plantlet regeneration.

show abstract

Induction of cassava somatic embryogenesis in liquid medium associated to floating membrane rafts

Abstract: ABSTRACT

Cited by 2 publications

References 19 publications

Indução de poliploidia em mandioca

Indução de poliploidia em mandioca

Somatic embryogenesis in two Nigerian cassava cultivars (Sandpaper and TMS 60444)

Contact Info

Product

Resources

About