Microbial production of citric acid

Vandenberghe, Luciana Porto de Souza; Soccol, Carlos Ricardo; Pandey, Ashok; Lebeault, J. M.

doi:10.1590/s1516-89131999000300001

Cited by 109 publications

(63 citation statements)

References 60 publications

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“…However, carbon dioxide has been shown to have a positive effect on the synthesis of citric acid (Vandenberghe et al 1999). The high partial pressure of carbon dioxide impedes the liberation of spores of the filamentous fungi, thereby enhancing citric acid accumulation.…”

Section: Aerationmentioning

confidence: 99%

Overview of citric acid production from Aspergillus niger

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Oladele

Siew

et al. 2015

Frontiers in Life Science

199

111

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Citric acid has high economic potential owing to its numerous applications. It is mostly produced by microbial fermentation using Aspergillus niger. In view of surges in demand and growing markets, there is always a need for the discovery and development of better production techniques and solutions to improve production yields and the efficiency of product recovery. To support the enormous scale of production, it is necessary and important for the production process to be environmentally friendly by utilizing readily available and inexpensive agro-industrial waste products, while maintaining high production yields. This article reviews the biochemistry of citric acid formation, choices of citric-acid producing microorganisms and raw materials, fermentation strategies, the effects of various fermentation conditions, citric acid recovery options and the numerous applications of citric acid, based on information drawn from the literature over the past 10 years.

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Section: Aerationmentioning

confidence: 99%

Overview of citric acid production from Aspergillus niger

Show

Oladele

Siew

et al. 2015

Frontiers in Life Science

199

111

View full text Add to dashboard Cite

show abstract

“…Citric acid fermentation starts from spores and their germination requires pH>5.0. The absorption of ammonia by germinating spores causes release of protons, thus lowering the pH (near 2.5) and improving the production of citric acid [12][13][14]. But the pH of fermentation media was not reduced below 4.72 and 3.35 in the media containing 200 and 160 g/L sugar, respectively, during fermentation, therefore citric acid production was not increased over 19.96 and 34.58 g/L respectively.…”

Section: Resultsmentioning

confidence: 99%

Citric Acid Production from Nontreated Beet Molasses by a Novel Aspergillus niger Strain: Effects of pH, Sugar and Ingredients

Guc¹,

Erkmen²

2017

J Food Microbiol Saf Hyg

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Effects of factors on the citric acid production from non-treated beet molasses were studied in Aspergillus niger OE55. Maximum amount of citric acid (19.13 and 34.62 g/L) was achieved when the initial pH of fermentation medium was 6.0 from 200 g/L and 150 sugar respectively. Citric acid production and biomass formation continuously increased during fermentation period in the media initially containing 200 g/L sugar. Remaining sugar (from 3.20 to 6.03 g/L) was higher at the end of fermentation in the media initially containing 160 g/L sugar than 200 g/L sugar. Yield of citric acid after 4 days of fermentation were ranged from 0.16 to 0.28 g/g from 160 g/L sugar. The high phosphorus and nitrogen levels stimulated biomass formation and reduced citric acid production. The optimum incubation for maximal citric acid production varies both with the sugar concentration in non-treated molasses and fermentation conditions for the novel A. niger strain. pH of fermentation media cannot have reduced below 4.72 and 3.35 in the media containing 200 and 160 g/L sugar, respectively, during fermentation, therefore citric acid production was not increased over 19.96 and 34.58 g/L respectively. This would be due to the formation of nitrogenous and polysaccharide compounds from molasses. Strain development in the citric acid production from wild strains depends mainly on the fermentation conditions.

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“…This strategy also caused our engineered cell factory to produce more than 36 g/L of citric acid as the major bioproduct which was nearly stable during the 4-day bioreactor fermentation. The production of this byproduct, which is extensively used in pharmaceutical industries [108] raises more revenue and offsets the total production cost. This Y. lipolytica platform can be tailored to produce LCDCA of higher specificity with respect to chain length and saturation.…”

Section: Discussionmentioning

confidence: 99%

Combinatorial Engineering of Yarrowia lipolytica as a Promising Cell Biorefinery Platform for the de novo Production of Multi-Purpose Long Chain Dicarboxylic Acids

2017

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This proof-of-concept study establishes Yarrowia lipolytica (Y. lipolytica) as a whole cell factory for the de novo production of long chain dicarboxylic acid (LCDCA-16 and 18) using glycerol as the sole source of carbon. Modification of the fatty acid metabolism pathway enabled creating a pool of fatty acids in a β-oxidation deficient strain. We then selectively upregulated the native fatty acid ω-oxidation pathway for the enhanced terminal oxidation of the endogenous fatty acid precursors. Nitrogen-limiting conditions and leucine supplementation were employed to induce fatty acid biosynthesis in an engineered Leu -modified strain. Our genetic engineering strategy allowed a minimum production of 330 mg/L LCDCAs in shake flask. Scale up to a 1-L bioreactor increased the titer to 3.49 g/L. Our engineered yeast also produced citric acid as a major by-product at a titer of 39.2 g/L. These results provide basis for developing Y. lipolytica as a safe biorefinery platform for the sustainable production of high-value LCDCAs from non-oily feedstock.

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Microbial production of citric acid

Abstract: ABSTRACT

Cited by 109 publications

References 60 publications

Overview of citric acid production from Aspergillus niger

Overview of citric acid production from Aspergillus niger

Citric Acid Production from Nontreated Beet Molasses by a Novel Aspergillus niger Strain: Effects of pH, Sugar and Ingredients

Combinatorial Engineering of Yarrowia lipolytica as a Promising Cell Biorefinery Platform for the de novo Production of Multi-Purpose Long Chain Dicarboxylic Acids

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