Newcastle disease virus vaccine strains: immunogenicity is not influenced by ICPI

Ma, Orsi; Júnior, Luciano Doretto; Reischak, Dilmara; Silva, Lha da; Spilki, Fernando Rosado; Buzinaro, MG; Cw, Arns

doi:10.1590/s1516-635x2009000200009

Cited by 18 publications

(10 citation statements)

References 7 publications

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“…The ICPI of CBU2179, CBU2249, and CBU2374 was 0.14, 0.33, and 0.39, respectively. ICPI values for VG/GA and B1 from previous studies were 0.03 and 0.2, respectively (Allan et al, 1978, Orsi et al, 2009. Also, no statistical differences in the ciliostasis index were found among the groups, except for the negative control, from which the index was lowest.…”

Section: Resultsmentioning

confidence: 70%

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Characterization of avian paramyxovirus type 1 from migratory wild birds in chickens

Shim

Won³

et al. 2011

Avian Pathology

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Newcastle disease virus (NDV) is one of the most important infectious agents in the poultry industry, and vaccines against it have been widely used for prevention and control. Live vaccines, which can replicate in the respiratory and digestive systems, have been especially needed in areas with outbreaks of viscerotropic velogenic Newcastle disease. Towards the goal of searching for a new live vaccine candidate, avian paramyxovirus type 1 (APMV-1) was isolated from the faeces of wild birds. Three APMV-1 strains thus isolated were characterized in terms of phylogeny, pathogenicity, immunogenicity and tissue tropism, and on the basis of these analyses were classified as lentogenic genotype I NDV. CBU2179, one of the three APMV-1 strains, was selected and was evaluated in terms of its efficacy and safety in specific pathogen-free chickens and commercial broilers. The manufactured trial vaccine from this strain, also called CBU2179, induced similar immune responses to those of VG/GA and B1 commercial vaccines, and provided 100% protection against challenge from viscerotropic velogenic NDV, KJW/49 strain (the official challenge strain in Korea). Also, the CBU2179 virus was re-isolated and persisted as long as or longer than other vaccine strains in both the respiratory and alimentary tracts. Therefore, the CBU2179 strain may represent a good candidate for a live Newcastle disease vaccine to protect chickens against viscerotropic velogenic NDV.

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Section: Resultsmentioning

confidence: 70%

“…There were no statistically significant differences in MDT test results among the strains. a Data from Orsi et al (2009). b Data from Allan et al (1978) and Alexander & Allan (1974).…”

Section: Discussionmentioning

confidence: 99%

Characterization of avian paramyxovirus type 1 from migratory wild birds in chickens

Shim

Won³

et al. 2011

Avian Pathology

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“…The highest dilution in which all embryos die was calculated as mean lethal dose [ 7 ]. Viral virulence was categorized by three classifications: Embryo mortality <60 h (Velogenic), 60-90 h (Mesogenic), and > 90 h (Lentogenic) [ 3 ].…”

Section: Methodsmentioning

confidence: 99%

“…The incidence of ND that attacks poultry shows manifestations of gastrointestinal, respiratory, and neurological disorders that cause deaths of up to 100% depending on the viral pathotype [ 1 , 2 ]. ND virus is highly contagious and causes huge economic losses in the poultry industry due to the decrease in production and quality of eggs and chicken performance [ 3 ]. Virulent of NDV strains are endemic to poultry in most of Asia, Africa, and some northern and southern states of America.…”

Section: Introductionmentioning

confidence: 99%

Effect of Newcastle disease virus level of infection on embryonic length, embryonic death, and protein profile changes

Qosimah¹,

Murwani²,

Sudjarwo³

et al. 2018

Vet World

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Background and Aim:Newcastle disease virus (NDV) is an obligate intracellular parasite. Virus can only live on living cells. The embryonated chicken eggs (ECEs) are one of the growth media of virus that is a cheap, easy to do, and accurate for showing patterns of virus change in the host. Higher virus titers indicate the higher number of viruses and more virulent to infect host. This research aimed to investigate the effect of different level of NDV titer infection in ECEs on protein profile, embryonic length, mortality, and pathological change.Materials and Methods:The study used a completely randomized design of six treatments and seven replications. The treatments were different level of NDV titer infection in allantoic fluid (AF) of 9-11 days ECEs, i.e., P1=20, P2=26, P3=27, P4=28, P5=29, and P6=210 hemagglutination unit (HAU). All samples were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Data were analyzed using one-way ANOVA with p=0.05 for length of the embryo and descriptive analysis for embryo mortality, pathology change, and protein band.Results:The result showed that protein profile of NDV-infected ECEs of all different levels is more complex than protein profile of no NDV-infected ECEs. NDV infected of all different levels showed longer size embryo, higher mortality embryo at the first 2 days, and higher occurrence of hemorrhagic in all part of bodies of embryo than those of no NDV infected.Conclusion:It was concluded that NDV infection of all different level decreased health conditions of chicken embryo of ECEs of 9-11 days old. Different level of NDV infection of ECEs of 9-11 days old showed no significantly different embryo profiles. However, all of the NDV-infected embryos were shorter, death on the 2nd day, and suffered more hemorrhage on all body surfaces than uninfected NDV embryos.

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“…The mortality rate of BP-caKII and K2 was measured by mean death time (MDT), and absolute lethal dose (ALD). MDT was measured as previously reported [ 16 , 17 , 18 ]. Briefly, BP-caKII and K2 were diluted within the range of 10 2 to 10 4 EID 50 /100 µL and 100 µL was injected into 10-day-old SPF embryonated eggs via the allantoic cavity.…”

Section: Methodsmentioning

confidence: 99%

Pathobiological and Genomic Characterization of a Cold-Adapted Infectious Bronchitis Virus (BP-caKII)

Hong

Lee

et al. 2018

Viruses

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We established a cold-adapted infectious bronchitis virus (BP-caKII) by passaging a field virus through specific pathogen-free embryonated eggs 20 times at 32 °C. We characterized its growth kinetics and pathogenicity in embryonated eggs, and its tropism and persistence in different tissues from chickens; then, we evaluated pathogenicity by using a new premature reproductive tract pathogenicity model. Furthermore, we determined the complete genomic sequence of BP-caKII to understand the genetic changes related to cold adaptation. According to our results, BP-caKII clustered with the KII genotype viruses K2 and KM91, and showed less pathogenicity than K2, a live attenuated vaccine strain. BP-caKII showed delayed viremia, resulting in its delayed dissemination to the kidneys and cecal tonsils compared to K2 and KM91, the latter of which is a pathogenic field strain. A comparative genomics study revealed similar nucleotide sequences between BP-caKII, K2 and KM91 but clearly showed different mutations among them. BP-caKII shared several mutations with K2 (nsp13, 14, 15 and 16) following embryo adaptation but acquired multiple additional mutations in nonstructural proteins (nsp3, 4 and 12), spike proteins and nucleocapsid proteins following cold adaptation. Thus, the establishment of BP-caKII and the identified mutations in this study may provide insight into the genetic background of embryo and cold adaptations, and the attenuation of coronaviruses.

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Newcastle disease virus vaccine strains: immunogenicity is not influenced by ICPI

Cited by 18 publications

References 7 publications

Characterization of avian paramyxovirus type 1 from migratory wild birds in chickens

Characterization of avian paramyxovirus type 1 from migratory wild birds in chickens

Effect of Newcastle disease virus level of infection on embryonic length, embryonic death, and protein profile changes

Pathobiological and Genomic Characterization of a Cold-Adapted Infectious Bronchitis Virus (BP-caKII)

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