Immunological evaluation of the intestinal mucosa of broiler chicks treated with Lactobacillus Spp. and challenged with Salmonella Enteritidis

Okamoto, Adriano Sakai; Filho, RL Andreatti; Lima, Edna Tereza de; Pereira, R. F.; Menconi, A.; Rocha, Ticiana Silva; Marietto-Gonçalves, GA

doi:10.1590/s1516-635x2007000400009

Cited by 11 publications

(7 citation statements)

References 6 publications

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“…Christensen et al, (2002) suggested that some of these effects were mediated by cytokines secreted by immune system cells stimulated with probiotic bacteria. On the other hand, Okamoto et al, (2007) demonstrated that this type of treatment had few beneficial effects for chicks, particularly during the first days of life.…”

Section: Results Listed In Tablementioning

confidence: 99%

A Trial to Prevent Salmonella Enteritidis Infection in Broiler Chickens Using Autogenous Bacterin Compared with Probiotic Preparation

El-Ghany¹,

El-Shafii²,

Hatem³

et al. 2012

JAS

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This study was carried out to investigate the efficacy of the locally prepared autogenous Salmonella Enteritidis (S. Enteritidis) bacterin as well as a probiotic preparation in the prevention of broiler chickens from S. Enteritidis infection. A total of three hundred and ten, one day-old Hubbard broiler chicks were used. At day old, ten chicks were sacrificed and examined bacteriologically to prove their freedom from S. Enteritidis infection. Three hundred birds were divided into four equal groups. Chickens in group (1) were kept as blank control negative non infected-non treated birds, while those of group (2) were challenged non treated birds. Group (3) was vaccinated intramuscularly by the autogenous bacterin at the first day of age in a dose of 0.2 ml/bird and boostered as a second dose at 10 days of age in a dose 0.5 ml/bird, however, group (4) was given a commercial probiotic preparation as 1gm/ 4 liter of the drinking water from the first day of age and continued for 5 successive days. All birds in groups 2, 3, and 4 were challenged orally by 0.5 ml containing 10 9 CFU/ml S. Enteritidis at 20 days of age. All the groups were kept under complete observation for three weeks for recording signs, moralities, gross lesions, shedding rate of S. Enteritidis, re-isolation of the organism, the performance as well as detection of the titer of antibodies serologically using microagglutination test and enzyme linked immunosorbant assay (ELISA) test. The results showed that the both the bacterin and the probiotic are equally effective in reducing signs, mortalities, gross lesions, the shedding rate and the re-isolation of S. Enteritidis and also increasing in the performance of chickens. The effect of the bacterin and the probiotic was significant (P≤0.05) when compared with the infected non treated chickens. Moreover, the serological investigation revealed an improvement in the titer of antibodies after vaccination and probiotic treatment. In conclusion, double doses of locally prepared autogenous S. Enteritidis bacterin and the probiotic preparation were effective and safe methods for prevention of S. Enteritidis infection in broiler chickens.

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Section: Results Listed In Tablementioning

confidence: 99%

A Trial to Prevent Salmonella Enteritidis Infection in Broiler Chickens Using Autogenous Bacterin Compared with Probiotic Preparation

El-Ghany¹,

El-Shafii²,

Hatem³

et al. 2012

JAS

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“…It was centrifuged at 3000 rpm for 10 min and the sediment was diluted with sterile phosphate buffer saline and adjusted to contain 10 9 CFU/mL using a McFerland matching tube to be used for challenging. According to the method of Timms [22], the challenge inoculum was prepared at 28 days of age, each bird in the challenged groups was infected orally with 0.5 mL suspension containing 10 9 CFU/mL SE [23]. The commercial balanced ration that met the broiler chicken requirements according to the National Research Council [24] was used as follows: starter (23% crude protein and metabolizable energy 3008 Kcal/kg), grower (21% crude protein and 3080 Kcal/kg diet) and finisher feed (19% crude protein and 3190 Kcal/kg diet) until 12, 26 and 42 days of age, respectively.…”

Section: The Challenge Bacteriummentioning

confidence: 99%

The Simultaneous Administration of a Probiotic or Prebiotic with Live Salmonella Vaccine Improves Growth Performance and Reduces Fecal Shedding of the Bacterium in Salmonella-Challenged Broilers

El‐Shall

Awad

El‐Hack

et al. 2019

Animals

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Salmonellosis is one of the most important bacterial diseases in poultry, causing heavy economic losses, increased mortality and reduced production. The aim of this study was the comparative efficacy of a commercial probiotic and/or prebiotic with a live attenuated Salmonella Enteritidis (SE) vaccine on the protection of broiler chickens from SE challenge. The efficacy of probiotic or prebiotic products, as well as a live Salmonella Enteritidis (SE) vaccine at the 7th day of age, administered via drinking water, were evaluated for clinical protection and effects on growth performance of broiler chickens experimentally challenged with SE at the 28th day of age. The use of probiotic or prebiotic simultaneously with the live Salmonella vaccine can diminish the negative effect of live vaccine growth performance, reducing mortality rate, fecal shedding, and re-isolation of SE from liver, spleen, heart and cecum. The use of probiotic or prebiotic simultaneously with the application of the live Salmonella vaccine is a good practice to diminish the negative effect of the harmful bacteria and improve the growth performance of broilers. Thus, further studies may be carried out with layers and breeders.

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“…The inoculum was prepared according to the method of Timms et al (1990). At 21 days of age, each bird in the experimentally infected groups was inoculated orally with 0.5 ml/ containing 10 9 CFU/ml S. Enteritidis (Okamoto et al, 2007).…”

Section: Experimental Infectionmentioning

confidence: 99%

Salmonella Enteritidis in Broiler Chickens: Isolation, Antibiotic Resistance Phenotyping and Efficacy of Colistin on Control of Experimental Infection

2020

Int J Vet Sci

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Out of 400 examined samples 45 suspected Salmonella isolates (11.25%) were obtained 19 (9.5%) out of apparently healthy and 26 (13%) from diseased chickens. Intestinal samples had more isolates (29, 14.5%) more than liver (16, 8%). Identified S.Enteritidis from suspected salmonella was 16/45 (35.6%) with a rate of 8% out of the examined 400 samples, 6 (3.0%) out of apparently healthy and 10 (5.0%) from diseased chickens. Intestinal samples had more isolates (11, 5.5%) than liver (5, 2.5%). The Antibiotics susceptibility profile of S. Enteritidis isolates revealed 100% resistance to trimethoprim/sulfamethoxazole, followed by oxacillin (62.5%), 56.3% for each of ampicillin, clindamycin, enrofloxacin and doxycycline, 50% for chloramphenicol, 43.8% for streptomycin, 37.5% to cephalosporins and 18.8% for colistin. Tested S. Enteritidis isolates are classified into 11 profiles and are resistant to two - nine antibiotic classes with resistant index 0.2- 0.9. Only two isolates are NDR (12.5%), most of isolates 10/16 (62.5%) are MDR and 25% are EDR to 8-9 antibiotics. Clinical signs in experimentally infected chickens appeared at 2nd dpi, mortality started at the 4th to reach 27.5% in infected nontreated and 5% in colistin treated. Signs and lesions were markedly severe in infected nontreated than treated. S. Enteritidis was re-isolated from dead infected birds. S. Enteritidis intestinal count in sacrificed infected nontreated was higher than treated. Colistin treated group showed higher FCR, EEF and CV% (1.52, 402.8 and 6.12%) than infected non-treated (1.73, 222.6 and 14.83%). It could be concluded that S. Enteritidis is prevalent in broiler chicken flocks. Most of the isolates are MDR. Experimental infection of broiler with S. Enteritidis field isolates resulted in high mortality and the addition of colistin sulphate in drinking water controlled the infection and restores the productivity of infected broiler chickens.

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Immunological evaluation of the intestinal mucosa of broiler chicks treated with Lactobacillus Spp. and challenged with Salmonella Enteritidis

Cited by 11 publications

References 6 publications

A Trial to Prevent Salmonella Enteritidis Infection in Broiler Chickens Using Autogenous Bacterin Compared with Probiotic Preparation

A Trial to Prevent Salmonella Enteritidis Infection in Broiler Chickens Using Autogenous Bacterin Compared with Probiotic Preparation

The Simultaneous Administration of a Probiotic or Prebiotic with Live Salmonella Vaccine Improves Growth Performance and Reduces Fecal Shedding of the Bacterium in Salmonella-Challenged Broilers

Salmonella Enteritidis in Broiler Chickens: Isolation, Antibiotic Resistance Phenotyping and Efficacy of Colistin on Control of Experimental Infection

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