2007
DOI: 10.1590/s1516-635x2007000300001
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Abstract: This experiment was designed with the objective of developing a simple, practical, and high repeatability technique for the simultaneous evaluation of the integrity of the plasmatic and acrosomal membranes, as well as funcional mitochondria of domestic fowl spermatozoa using an association of fluorescent probes. Four ejaculates (motility > 80% and abnormal morphology < 10%) from each of six Ross male broiler breeder (n=24) were diluted in TALP sperm medium (25x10(6) spermatozoa/mL) and split into two aliquots,… Show more

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Cited by 11 publications
(6 citation statements)
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“…Triple stain validation. For the purpose of optimizing the triple stain for dog sperm, semen samples of 7 dogs were collected and diluted in TALP sperm medium, in order to reach the final concentration of 25 million cells per mL [25]. Diluted samples were divided into two aliquots: sperm kept at 37 • C (considered as intact sperm) and sperm submitted to snap-freezing (considered as damaged sperm).…”
Section: Simultaneous Assessment Of Plasmatic Acrosomal and Mitochond...mentioning
confidence: 99%
“…Triple stain validation. For the purpose of optimizing the triple stain for dog sperm, semen samples of 7 dogs were collected and diluted in TALP sperm medium, in order to reach the final concentration of 25 million cells per mL [25]. Diluted samples were divided into two aliquots: sperm kept at 37 • C (considered as intact sperm) and sperm submitted to snap-freezing (considered as damaged sperm).…”
Section: Simultaneous Assessment Of Plasmatic Acrosomal and Mitochond...mentioning
confidence: 99%
“…It is possible to suggest that the application of this methodology in large scale can be maximized by the use of flow cytometry systems, providing higher accuracy and swiftness, as it is to read approximately 10,000 cells in a few seconds (Celeghini et al, 2007).…”
Section: Acrosomal Integrity (Fitc-psa)mentioning
confidence: 99%
“…Diluent and cryoprotectant choice for horse semen cryopreservation is under intense investigation [12,18,30,38], aiming to reduce toxicity and osmotic stress during cryopreservation [12,14,26]. Several parameters are used to measure horse semen cryotolerance, such as sperm motility [1,2], sperm membrane integrity and acrosome and mitochondria viability [8,30], zona pellucida binding assays [27,36], and incidence of DNA fragmentation [20,32]. Based on the facts above, the interplay between horse breed and cryopreservation protocol plays a major role on semen cryotolerance in horses.…”
Section: Introductionmentioning
confidence: 99%