Antibacterial efficacy of commercial disinfectants on dirt floor used in poultry breeder houses

Abstract

Dirt floors are used on most Brazilian poultry farms since the construction of concrete floors is very expensive. In vitro tests carried out to verify the effectiveness of disinfectants do not consider the adverse conditions found in poultry farms. Therefore, the present study aimed at evaluating the effect of six commercial disinfectants on the reduction of total and fecal coliforms on the dirt floor of breeder houses. The amount of disinfectant solution to be used per square meter was defined by counting total and fecal coliforms at different soil depths and by analyzing soil physical properties. Coliforms were detected at 0.5 cm, and one liter of disinfectant solution was sufficient for soil saturation at this depth. After that, the efficacy of six commercial products (caustic soda, hydrated lime, phenols 1 and 2, iodine, glutaraldehyde, and quaternary ammonium) in reducing the number of coliforms, after six hours of contact with the dirt floor, was assessed using the most probable number (MPN) method. Escherichia coli specimens isolated from the dirt floor were used to evaluate in vitro effectiveness of disinfectants. Products that yielded the best results in the MPN method were also effective in the in vitro tests. Among the tested disinfectants, hydrated lime was the most efficient, reducing the initial contamination by 2.9 log after six hours of contact with the dirt floor

Development of a method of protection of concrete floors of animal buildings from corrosion at the expense of using dry disinfectants

“…Poultry disinfection programs use hydrated lime to provide more light intensity for the birds and improve the appearance of the farm. However, according to this study, hydrated lime was more effective than currently available disinfectants [12]. Unfortunately the use of slaked lime is possible for walls, but not practical for use on concrete floors.…”

Evaluation of the efficiency of the most common commercial disinfectants against some pathogens isolated from New-valley poultry farms "in-vitro studies."

“…At time intervals, 5, 15, and 30 minutes, 1 ml was taken into tubes containing 9 ml. saline and the colony count was conducted by the pour plate technique (Pilotto et al, 2007). Each disinfectant was tested against the disinfectants at three different concentrations including the recommended concentrations as well as a higher and lower concentration (Table 1).…”