Rotavirus detection and isolation from chickens with or without symptoms

Abstract

Rotaviruses have been identified as one of the main etiological agents of diarrhea and enteritis in mammals, including humans, and in avian species. Few studies have been published about enteric viruses in Brazilian poultry, including those related to rotavirus infection. Such studies demonstrate significant occurrence and the importance of enteric viruses in poultry presenting intestinal problems. Enteric viruses are the primary cause of injuries to the gut, allowing other agents, especially bacteria, to attach, to penetrate, and to replicate in the enteric tissue, leading to further damage. The aim of the present study was to detect rotavirus in the intestinal contents of layers and broilers by polyacrylamide gel electrophoresis (PAGE) and virus isolation in MA-104 cell culture. A total of 45.3% of all samples were positive to rotavirus; rotavirus frequencies were 48.7% in samples from flocks with diarrhea, 46.4% in flocks with delayed growth, and 30% in asymptomatic flocks. It was possible to isolate rotavirus in MA-104 cells from the nine rotavirus-positive randomly chosen samples. These results indicate that rotavirus may have an important role in pathogenesis of enteric disease

Molecular Epidemiologic Study on Rotavirus Infection in Human and Birds in Association With Gastroenteritis

“…A total of 251 diarrhoeic feces of birds 1 to 24 days of age were examined by PAGE -SS method, of which 33 birds (13.15%) had rotavirus infection, but higher rate of rotavirus infection in birds was recorded 45.3% by Villarreal et al, (2006). He reported that 48.7% rotavirus frequencies among samples from flocks with diarrhoea, 46.4% among flocks with delayed growth, and 30% among asymptomatic flocks.…”

Avian rotavirus enteritis – an updated review

“…Turkey poults, for example, have been often infected during their first week of life while broiler chickens were generally found infected at about four weeks of age . RVD, RVF and RVG have been seen exclusively in poultry (Urasawa et al 1992;Saif & Jiang 1994;Santos & Hoshino 2005;Kattoor et al 2013a;Kattoor et al 2013b) and may contribute to disease (McNulty et al 1984;McNulty 2003;Villarreal et al 2006). The D group has also been frequently detected in avian species.…”

Detection of rotavirus VP7 gene in helmeted guinea fowls and Japanese quails in Ogun state, Nigeria

“…It also causes decreased weight gain, increased morbidity, increased mortality and increased production costs due to poor feed conversions. Cumulatively, all these can lead to huge economic losses in poultry production Villarreal et al, 2006) and the potential economic resources of the poultry industry may not be fully utilized until the etiological agent of diseases are recognized and possibly controlled. Over 70% of Nigeria's population keeps different types of birds as backyard poultry, where they serve as source of protein and income to the owners (Adene & Oguntade, 2006).…”