Experimental infection by Salmonella enterica subsp enterica serovar kottbus in day-old broiler chickens

Abstract

We previously described Salmonella enterica serovar Heidelberg isolates harboring a chromosomal gene cluster similar to the glutathione S-transferase gene, a putative fosA gene conferring resistance to fosfomycin. Here, we show that this new gene, named fosA7, confers resistance to fosfomycin. The introduction of fosA7 into the fosfomycin-susceptible Salmonella enterica serovar Enteritidis resulted in a substantial increase in the fosfomycin MIC. This finding increases the awareness of antibiotic resistance in Salmonella Heidelberg from broilers as related to the food safety and public health.

Experimental infection of one-day-old chicks with Salmonella Serotypes Previously isolated from poultry facilities, wild birds, and swine

“…On 21 dpi, Salmonella serotypes were present in all 72 (100%) cecal content samples, but in the organs of only 16 (11.8%) birds. These results agree with the observations of Ribeiro et al (2005), who investigated S. Kottbus in one-day-old chicks.…”

“…to investigate the pathogenicity of this agent (Pinheiro et al, 2001;Oliveira et al, 2005;Ribeiro et al, 2005).…”

“…in specimens collected from poultry facilities, local wild birds, and swine reared on the same farm. After the collected bacteria were cultured, broiler chicks were inoculated with the isolated serotypes and evaluated for disease (Pinheiro et al, 2001;Oliveira et al, 2005;Ribeiro et al, 2005).…”

Examination of the Expression of Immunity Genes and Bacterial Profiles in the Caecum of Growing Chickens Infected with Salmonella Enteritidis and Fed a Phytobiotic

“…Infection of the birds was performed intramuscularly according to the standard procedure [69], with the aim to induce an immune response as normally expected by an intramuscular vaccination and shown to be efficient in other studies [70,71,72,73,74]. The challenge dose used in this experiment was in agreement with that employed in the ARVRIPS and in other studies [75,76,77]. The initiation of the inflammatory process in chickens after infection with a SE strain was confirmed based on the analysis of the content of nitrite and nitroso compounds in the blood [69,78].…”

The Role of Synbiotics (Commercial Product) as a Substitute for Antibiotic Growth Promotor (AGP) in the Performance and Blood Values of Cobb-strain Broilers Challenged with Salmonella enteritidis

“…Salmonella enteritidis can cause nutritional malabsorption, decreased growth performance, and inflammation in internal organs which lead significant economic losses in the poultry industry, in addition to contaminated poultry products and diseases carried by poultry feeds. Salmonella enteritidis has a predilection for intestinal villous epithelium, shows the presence of specific receptors, and, within 24 hours, will have reached the lamina propria and then the infiltration of inflammatory cells, thickening of the intestinal mucosa [29,30]. Some factors that influence the course of Salmonella infections include the chicken's immune response to bacterial infections, number of bacterial doses, and presence of immunosuppressive diseases [31].…”