Elisa evaluation of the levels of antibodies against Infectious Bronchitis Virus in laying hens using egg yolk as substrate

Abstract

The present work was carried out to compare Infectious Bronchitis Virus (IBV) antibody titers in serum and egg yolk samples from laying hens. Sixty paired blood and egg samples were collected from laying hens of two farms. Serum samples were frozen, while egg yolk samples were diluted (1:500) before freezing. Serum and yolk samples were tested for the presence of IBV antibodies by indirect ELISA (commercial kit) and titers were compared by a correlation test (alpha=0.05). There was a high correlation (r=0.62) between the two kinds of samples, which means that titers of IBV antibodies in the egg yolk and in serum samples are quite the same. Considering that blood collection causes deep stress that leads to economic losses, and since eggs are collected daily on the farm, results reported here are of importance to poultry production

Effect of hyperimmunized egg yolk on maternal immunity of Newcastle disease vaccine in broiler chicks

“…Vaccination for protecting chicken from ND is routinely practiced throughout the world, but the vaccination is ineffective with the high titer of maternal antibodies (2,3). In birds the maternal antibodies in the egg is manifested by three classes of antibodies in chicken namely IgY, IgA and IgM (4-6), IgY is present in the egg yolk whereas IgA and IgM are present in the egg white as a result of mucosal secretion in the oviduct, the transfer of IgY from the dam to her offspring takes place in a two step process in the first step IgY is taken up into the egg yolk by IgY receptors on the ovarian follicle from the dam's blood through the granulosa cells in the second step IgY is transferred from the egg yolk to the offspring via the embryonic circulation (4,(6)(7)(8). This type of immunoglobulin is act as passive immunity because these antibodies are derived from the dam and protect the offspring from various infectious disease (9).…”