Patogenicidade In Vivo e In Vitro de Amostras de Escherichia Coli de Origem Aviária

Assis, Acb; Santos, B. M.

doi:10.1590/s1516-635x2001000200007

Cited by 3 publications

(3 citation statements)

References 12 publications

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“…The in vivo pathogenicity test was performed with an inoculum prepared from the culture of E. coli samples in brain-heart broth (BHI) (Oxoid, England). Inoculation was performed through air sac, using for each isolate seven one day-old chicks, from which ϐive were inoculated with 0.1ml of bacterial suspension at the approximate concentration of 10 7 colony-forming units (CFU/ mL) and two control, inoculated with 0.1ml of BHI broth (Assis & Santos 2001). Inoculated animals were observed every 12 hours for mortality up to 72 hours after inoculation.…”

Section: Isolation Identiϐication Escherichia Coli Pathogenicity Anmentioning

confidence: 99%

Research of Salmonella spp. and evaluation of pathogenicity, cytotoxicity of Escherichia coli isolates proceeding from sparrows (Passer domesticus)

et al. 2012

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Pesq. Vet. Bras. 32(9) The aim of this study was to research the occurrence of Salmonella spp. and Escherichia coli in feces samples of sparrows, as well as to identify the pathogenicity, cytotoxicity and sensitivity proϐile of the isolates to antimicrobial use. Two hundred and twenty eight sparrows were captured in eight farms. The in vitro pathogenicity test was performed by the isolates culture on congo red-magnesium oxalate Agar, whilst the in vivo pathogenicity test was performed in one day-old chicks. In order to study the cytotoxic effects of indicators, samples were inoculated into Vero cells. The results obtained for Escherichia coli isolation conϐirmed the presence of this microorganism in 30 (13.2%) of the evaluated samples. Out of those isolates, 10 (33.3%) presented the capacity of absorbing ongo red. As for in vivo pathogenicity a 68.0% of mortality rate of the evaluated samples was observed. Out of 20 isolates tested for cytotoxin production, none of them presented cytotoxic effect in the Vero cells. The Salmonella spp was isolated only in one sample (0.04%), and it was identiϐied as Salmonella enterica subspecies houtenae. Results obtained through this research indicate the need for new studies to identify other virulence factors of E. coli samples and to delineate the phylogenetic proϐile of the isolates in order to establish a relation with colibacillosis outbreaks in chickens and broilers in the studied region, as well as to analyze the critical points in the aviculture productive chain to identify the source of Salmonella enterica subspecies houtenae.

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Section: Isolation Identiϐication Escherichia Coli Pathogenicity Anmentioning

confidence: 99%

Research of Salmonella spp. and evaluation of pathogenicity, cytotoxicity of Escherichia coli isolates proceeding from sparrows (Passer domesticus)

et al. 2012

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“…As aves podem ser reservatórios de inúmeros agentes etiológicos de doenças importantes para o homem e para os animais, como Salmonella spp., Escherichia coli, Influenza spp., Aspergillus spp., entre outros (Assis & Santos, 2001, Lima et al, 2001, Silva & Duarte, 2002, Ibiapina et al, 2005. Alguns desses agentes patogênicos são oportunistas, ou seja, convivem bem com seus hospedeiros até que um elemento de imunocomprometimento do hospedeiro permita sua multiplicação e, consequentemente, lhes causem danos orgânicos irreparáveis.…”

Section: Introductionunclassified

Megabacteriose aviária: breve revisão

Silva

Lallo

Bentubo

2022

RSD

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Megabacteriose constitui designação genérica aplicada a uma importante doença infecciosa aviária, inicialmente diagnosticada em periquitos, canários e avestruzes, mas que nos últimos anos tem sido observada em outras espécies, como mamíferos. Apesar dos esforços da ciência, a classificação taxonômica do microrganismo causador da desordem ainda não está bem estabelecida. A existência de indivíduos assintomáticos sugere que os reservatórios naturais desempenham um importante papel na disseminação da infecção e contribui para a controvérsia a respeito do papel do microrganismo como um patógeno primário. Inúmeras técnicas têm sido experimentalmente testadas no sentido de produzir maior sensibilidade e especificidade para o diagnóstico da doença. Embora a proventriculite não seja exclusivamente causada por Macrorhabdus ornithogaster, ela costuma ser o sinal clínico mais comumente associado à megabacteriose. Aparentemente, os macrolídeos poliênicos tem sido empregados com sucesso no tratamento da doença. Contudo, novos estudos devem ser estimulados para que esses aspectos sejam devidamente esclarecidos promovendo, assim, consenso entre clínicos e laboratórios.

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“…Salmonella can also be related to damages to the intestinal health (Assis & Santos, 2001) or determine only sub-clinical infections (Gast & Benson, 1995) and insidiously propagate to other animal species and result in a contaminated product and a potential problem to public health. In this context, lactulosis appears as an alternative to growth promoters, which improves animal health, modifying the balance of the intestinal microbiota and avoids colonization of pathogens, such as S. Typhimurium.…”

Section: Introductionmentioning

confidence: 99%

Performance of broilers experimentally inoculated with Salmonella Typhimurium and fed diets with addition of lactulosis

et al. 2012

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-The objective of this experiment was to evaluate the influence of lactulose on performance as well as its ability to prevent colonization by Salmonella Typhimurium in broilers orally inoculated with this pathogen. The design adopted was completely randomized, with 630 one-day-old male chicks distributed into six treatments, with seven replications and 15 birds per experimental unit. The treatments comprised the following procedures: T1 (control group) -no S.Typhimurium inoculation or supply of lactulosis; T2 -only inoculation of S. Typhimurium; T3 -only lactulosis supply; T4 -supply of lactulosis and S. Typhimurium inoculation on the first day of life; T5 -supply of lactulosis 48 hours before S.Typhimurium inoculation; and T6 -supply of lactulosis 48 hours after inoculation of S. Typhimurium. Performance variables were evaluated on the seventh, 14th, 21st and 28th days of age; fragments of the duodenum and jejunum were collected and sent to histomorphometric assessment at 14 days of age, and S. Typhimurium excretion was verified in cloacal swabs on the 10th, 24th and 35th days of age. Performance data were analyzed by ANOVA and Tukey's test (5%) and fecal excretion data were assessed by non-parametric chi-square test. Better weight gain and feed conversion were observed in groups fed lactulosis with or without challenge of S. Typhimurium up to 21 days of age. Reduced duodenum villous height was verified on the 14th day in groups challenged with the pathogen. Reduction of S. Typhimurium fecal excretion was verified in broilers fed lactulosis from the first day of life on and 48 hours before receiving S. Typhimurium directly into the crop. Lactulosis increases broiler performance up to one week after its inoculation, influences duodenum villous height and reduces the fecal excretion of Salmonella Typhimurium.

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Patogenicidade In Vivo e In Vitro de Amostras de Escherichia Coli de Origem Aviária

Cited by 3 publications

References 12 publications

Research of Salmonella spp. and evaluation of pathogenicity, cytotoxicity of Escherichia coli isolates proceeding from sparrows (Passer domesticus)

Research of Salmonella spp. and evaluation of pathogenicity, cytotoxicity of Escherichia coli isolates proceeding from sparrows (Passer domesticus)

Megabacteriose aviária: breve revisão

Performance of broilers experimentally inoculated with Salmonella Typhimurium and fed diets with addition of lactulosis

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