Effects of BST and high energy diet on gene expression in mammary parenchyma of dairy heifers

Lew, Betina Joyce; Oliveira, Mauro Dal Secco de; Júnior, José Esler de Freitas; Carvalho, Marina Vieira de; Rêgo, Aníbal Coutinho do; Rennó, Francisco Palma

doi:10.1590/s1516-35982013000700008

Cited by 2 publications

(5 citation statements)

References 32 publications

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“…Most IGF-1 molecules found in the circulation are bound with high affinity to one or more of the six known IGF-1 binding proteins (IGFBP-1–6) that modulate the bioavailability of IGF-1 in target tissues 39 . Previous studies have observed that rbST strongly influences the upregulation of IGFBP-5 in skeletal muscle of cattle 31 and downregulates IGFBP-3 in mammary tissue 32 . However, in this study, transcripts of neither IGFBP-3 nor IGFBP-5 were detected at any time point.…”

Section: Resultsmentioning

confidence: 90%

“…The final aim of this work was to propose a routine panel of genes whose combined transcription pattern would allow the development of a screening method to control the misuse of rbST in dairy farms via MSCs. Unlike other previous studies 27 , 31 , 32 , this work analysed the transcription patterns of rbST-related genes in a 8-month real-conditions experiment including 12 cycles of rbST administration and control animals. This approach permitted more accurate data to be obtained in order to differentiate between rbST-treated cows and control cows.…”

Section: Resultsmentioning

confidence: 99%

“…The expression of 18 genes (Table 1 ) in MSCs was evaluated by real-time PCR. The selection of those genes was mainly based in previous transcriptomic studies of cows treated with rbST and anabolic agents 26 , 27 , 31 , 32 . Of these 18 genes, three were used as endogenous controls to calculate the relative expression of the other 15 candidate genes.…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Tracing recombinant bovine somatotropin ab(use) through transcriptomics: the potential of bovine somatic cells in a multi-dose longitudinal study

Lamas

Regal

Vázquez

et al. 2019

Sci Rep

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In the European Union, the use of recombinant bovine somatotropin (rbST) in dairy cattle is forbidden. Monitoring rbST (ab)use by its direct detection in animal matrices still remains a challenging task. New monitoring methods based on indirect detection of the substance are necessary. A new transcriptomic system based on the use of high-throughput real-time PCR in combination with somatic cells was developed to control rbST administration in dairy animals. A total of nine cows, separated into control and rbST-treated groups, were included in the study. A subcutaneous injection containing 500 mg of rbST was administered to the treated group every 14 days, up to a total of 12 doses. Milk somatic cells (MSCs) were sampled from each animal at different time points throughout 8 months of study. It was possible to obtain the transcriptomic profile of 18 genes in MSCs of rbST-treated and control groups, and using univariate and multivariate statistical analysis control and treated animals were discriminated. The transcription of CCND1, IGF-1R, TNF and IL-1β genes resulted strongly influenced by rbST treatment. The combination of MSCs, transcriptomic tools and statistical analysis has allowed the selection of four genes as potential biomarkers that could be used in a transcriptomic panel for monitoring rbST administration in cows.

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Section: Resultsmentioning

confidence: 90%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Tracing recombinant bovine somatotropin ab(use) through transcriptomics: the potential of bovine somatic cells in a multi-dose longitudinal study

Lamas

Regal

Vázquez

et al. 2019

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Three genes were used as endogenous controls to calculate the relative expressions of the other 15 candidate genes. The selection of reference and target genes was based on results observed in previous transcriptomics studies, where the administration of rbST and anabolic agents to cattle was evaluated [ 13 , 14 , 15 , 16 , 17 , 31 ]. Gene-expression assays were carried out with a TaqMan ® OpenArray ® system (Applied BiosystemsTM, Thermo Fisher Scientific), involving a nanoliter high-throughput real-time PCR platform where 3072 reactions were performed simultaneously in the same OpenArray ® plate, and the primers and TaqMan ® probes were preloaded in the plates by the company.…”

Section: Methodsmentioning

confidence: 99%

“…Moreover, some studies used qPCR to develop a screening panel that could elucidate the use of growth promoters in cattle [ 13 , 14 ]. Some studies evaluated the gene-expression modifications caused by rbST in various tissues of dairy cattle [ 15 , 16 , 17 ]. However, none of these studies focused on the development of a screening panel to be used routinely in monitoring the illicit use of rbST in dairy farms.…”

Section: Introductionmentioning

confidence: 99%

Tracing Recombinant Bovine Somatotropin Ab(Use) Through Gene Expression in Blood, Hair Follicles, and Milk Somatic Cells: A Matrix Comparison

et al. 2018

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The use of recombinant bovine somatotropin (rbST) in dairy cattle is forbidden in the European Union. Due to the very low circulating concentration of rbST in treated animals, its direct detection is still a challenge. Therefore, the use of indirect methods to detect the ab(use) of rbST in dairy cattle appears as a good alternative. In the past few years, gene expression demonstrated its utility in screening the use of illicit substances in both humans and animals. In this study, a comparison of three types of matrices (milk somatic cells, blood, and hair follicles) was carried out to evaluate their potential use for routine control of rbST using 15 gene-expression profiles. A total of six rbST-treated cows and three control cows were included in the study. A subcutaneous injection containing 500 mg of rbST was administered to the treated group. Samples of the three matrices were collected before rbST administration, and at three and nine days after treatment. The quality of RNA extracted was higher in the blood and hair-follicle samples than in the milk somatic cells. In the three matrices, there were significant differences in the expression of some genes, with milk somatic cells and blood presenting the the best matrices. On this note, the cyclin D1 (CCND1), interleukin 1 beta (IL-1β), tumor necrosis factor (TNF), and insulin-like growth factor 1 receptor (IGF-1R) genes showed potential as biomarkers of rbST treatment. Therefore, blood, somatic cells, and follicle hair should be considered as promising sources of RNA, and can be used in gene-expression assays to routinely control the illicit use of rbST.

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Effects of BST and high energy diet on gene expression in mammary parenchyma of dairy heifers

Cited by 2 publications

References 32 publications

Tracing recombinant bovine somatotropin ab(use) through transcriptomics: the potential of bovine somatic cells in a multi-dose longitudinal study

Tracing recombinant bovine somatotropin ab(use) through transcriptomics: the potential of bovine somatic cells in a multi-dose longitudinal study

Tracing Recombinant Bovine Somatotropin Ab(Use) Through Gene Expression in Blood, Hair Follicles, and Milk Somatic Cells: A Matrix Comparison

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