Assessment of the interaction between straw size and thawing rate and its impact on in vitro quality of post-thaw goat semen

Bezerra, Francisco Silvestre Brilhante; Castelo, Thibério de Souza; Santos, E.A.A.; Dantas, Tiago da Costa; Simão, Bruno Rodrigo; Silva, Alexandre Rodrigues

doi:10.1590/s1516-35982012000300016

Cited by 8 publications

(5 citation statements)

References 26 publications

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“…Nearly similar results in goat was detected by Bezerra et al (2012) who recorded optimal results for progressive motility were achieved when goat semen was frozen in 0.5 mL straws and thawed in water at 55 ºC for 7 sec. On the other hand, studies have demonstrated that a fast thawing rate results in better post-thaw quality when compared with slower thawing for stallions, rams (Watson, 1990), bulls (Pace et al, 1981), boars (Eriksson andRodriguez-Martinez, 2000).…”

Section: Discussionsupporting

confidence: 68%

Effect of Adding Equex STM Paste and BHT to Different Extenders on Viability, Plasma Membrane and Dna Integrity of German Shepherd Dog Spermatozoa During Freezing and Thawing Methods in Egypt

2015

Assiut Veterinary Medical Journal

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Many factors influencing semen quality through all steps of cryopreservation such as semen processing, the combination of extender, cooling rate during the freezing procedure and the thawing technique. The present study was conducted to evaluate the potential cryoprotective effect of Equex STM paste and / or butylated hydroxytoluene (BHT) occlusion in Tris and milk based extenders, freezing according to 2 methods, thawing at 2 regimes, and the interactions between these treatments, on the post-thaw semen quality. A total number of 80 ejaculates was collected from 15 German shepherd stud dogs. Semen samples was frozen in two different extenders, Tris (TCF) and milk (SMG) based extenders, with or without 0.5% Equex STM paste and / or 1mM BHT compared with commercial bovine tris based semen extender (BULLXcell ™ , IMV technologies, Franc) using 2 freezing protocols (4 and 6 cm) above LN 2 and 2 thawing rates (45 o C for 30 sec. and 55 o C for 5 sec.). Post-thaw motility, viability, plasma membrane integrity in terms of post-thaw live spermatozoa, acrosomal defects, acrosome reaction and rate of increase in activity of extracellular AST, ALT and ALP enzymes as well as assessment of sperm DNA integrity using comet assay were recorded. The results clarified that addition of Equex STM paste and / or BHT to the TCF and SMG extenders significantly (P<0.01) improved post-thaw motility, viability, plasma membrane integrity and reduced acrosomal defects, acrosomal reaction, release of extracellular AST, ALT and ALP enzymes and DNA damage. The best post-thaw semen qualities was observed when freezing in a Styrofoam box above 6 cm highest above LN 2 and thawing at 55 o C for 5 sec. the superior results were obtained with Tris based extenders than milk based extenders. It is concluded that fortification of 0.5% Equex STM paste and / or 1mM BHT in Tris based extenders freezing above 6 cm highest above LN 2 and thawing at 55 o C for 5 sec. can improve the post-thaw dog semen motility, viability and plasma membrane integrity. Also, the commercial bovine extender BULLXcell™ is useful for cryopreservation of dog semen.

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Section: Discussionsupporting

confidence: 68%

Effect of Adding Equex STM Paste and BHT to Different Extenders on Viability, Plasma Membrane and Dna Integrity of German Shepherd Dog Spermatozoa During Freezing and Thawing Methods in Egypt

2015

Assiut Veterinary Medical Journal

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“…Furthermore, previous rabbit studies have shown that the size of the straw seemed to have no effect on rabbit fertility or prolificacy (Mocé et al, 2010). It is worth mentioning that Johnson et al (1995) and Bezerra et al (2012) have documented the interaction between straw size and thawing rate, as well as their impact on post-thaw semen parameters and fertility. This means that each type of straw would require a different thawing temperature and, as a result, a different time duration (Mocé et al, 2010).…”

Section: Discussionmentioning

confidence: 94%

Cryopreservation of rabbit semen: impacts of permeable and non-permeable mixture of cryoprotectant, male group individuality, freezing rate, semen package size and antioxidant bovine serum albumin on rabbit semen freezability

et al. 2022

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In the present study, three experiments were designed to identify the most appropriate technique for freezing rabbit semen. Experiment 1 aimed to determine the optimal levels of dimethyl sulfoxide (DMSO) contents in freezing medium and their effects on individual bucks. Semen ejaculates for each buck (n=15 bucks) were mixed and split into three portions for extension with a freezing medium containing varying concentrations of DMSO (0.75, 1.0, and 1.4 M). Diluted semen samples were packaged in 0.25 mL straws and suspended above liquid nitrogen (LN) for 10 min, then dipped in LN. A few days after freezing, post-thaw semen evaluation was assessed, and according to the results, six bucks and an extender containing 0.75 M of DMSO were used for experiments 2 and 3. In experiment 2, the pooled semen from 6 bucks was divided into two portions for packaging in two straw sizes (0.25 and 0.50 mL). Each straw size was divided into five groups and suspended at different heights above LN (2, 4, 6, 8, and 10 cm) for 10 minutes before being preserved in LN. In experiment 3, the pooled semen was divided into four portions for dilution with freezing medium containing different concentrations of bovine serum albumin (BSA; 0, 2.5, 5.0, and 7.5 mg/mL). Semen samples were packaged in a 0.50 mL straw and suspended 10 min, 4 cm above LN for freezing. Pre-freezing and post-thawing, semen samples were evaluated for semen quality. Results showed that the extender containing 0.75 M DMSO had higher significant values for post-thaw sperm motility, longevity, acrosome integrity and sperm plasma membrane permeability. Bucks’ individuality had significant effects on post-thaw motility, acrosome and sperm plasma membrane integrity. A significant interaction was recorded between DMSO concentrations and bucks’ individuality on sperm longevity. Semen package sizes had no significant effects on the evaluated parameters. Semen was frozen at 2 and 4 cm above LN had significantly better post-thaw quality. BSA at concentrations 5 and 7.5 mg/mL improved recovery rates of acrosome integrity and sperm membrane permeability. DMSO 0.75 M and freezing 4 cm above LN seem to be more adequate for rabbit semen cryopreservation. The appropriate level of DMSO differs between bucks, as the post-thaw sperm longevity is affected. BSA enhanced acrosome and sperm membrane integrity. Results obtained will need further investigation to be confirmed in the field.

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“…In reference to intermediate thawing temperature (50 °C), this did not show better results than the control sample (37 °C, 20 s), maybe because the rate obtained with this temperature avoids an adequate reduction of the size of the crystals during the recrystallization, causing severe damage to sperm [ 44 ]. The movement pattern observed in the samples thawed at 50 °C was marked by high values of velocities and ALH, parameters related to the sperm hyperactivation [ 45 ], which might be linked to a lower thawing velocity when pass through ‘warm shock’ phase, which might imply a higher calcium influx into the sperm cell and consequent hyperactivation [ 46 ].…”

Section: Discussionmentioning

confidence: 99%

Optimization of the Thawing Protocol for Iberian Boar Sperm

Tomás-Almenar¹,

Mercado²

2022

Animals

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Thawing protocols have been barely studied, and their modifications may lead to a substantial improvement in post-thawing sperm quality, which could be of great relevance to existing sperm banks, such as those for Iberian pig breeds with varieties in danger of extinction. For that, the study aimed to evaluate different thawing rates and to evaluate modifications in the composition of the thawing extender (basic pH to 8–8.2, incorporation of cyclodextrins loaded with cholesterol [CLC] and the incorporation of ion chelators [EDTA and EGTA]). After thawing, overall sperm motility and kinematic parameters, acrosome status and sperm membrane integrity were evaluated. The most optimal results were obtained with the thawing rate reaching 70 °C for 8 s with the inclusion of 12.5 mg of CLC/500 × 106 spermatozoa in the thawing extender, which showed an improvement compared to the control at 70 °C. In conclusion, to adapt the thawing conditions may be relevant, especially for endangered species or breeds such as some varieties of Iberian pig, since this process could also be used in samples cryopreserved in gene banks.

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Assessment of the interaction between straw size and thawing rate and its impact on in vitro quality of post-thaw goat semen

Cited by 8 publications

References 26 publications

Effect of Adding Equex STM Paste and BHT to Different Extenders on Viability, Plasma Membrane and Dna Integrity of German Shepherd Dog Spermatozoa During Freezing and Thawing Methods in Egypt

Effect of Adding Equex STM Paste and BHT to Different Extenders on Viability, Plasma Membrane and Dna Integrity of German Shepherd Dog Spermatozoa During Freezing and Thawing Methods in Egypt

Cryopreservation of rabbit semen: impacts of permeable and non-permeable mixture of cryoprotectant, male group individuality, freezing rate, semen package size and antioxidant bovine serum albumin on rabbit semen freezability

Optimization of the Thawing Protocol for Iberian Boar Sperm

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