Isolation and expeditious morphological, biochemical and kinetic characterization of propolis-tolerant ruminal bacteria

Abstract

-It was aimed in this work to evaluate bacterial strains tolerant to products based on propolis (LLOS) through the isolation, morphological and biochemical characterization techniques in diets with roughage:concentrate ratio 100:0 and 50:50. For roughage diets, the products LLOSC1 and LLOSB3 were evaluated, and for 50:50% diets, the products LLOSC1, LLOSD1, LLOSA2, and LLOSC3, which differed in alcoholic concentrations (1, 2 and 3) and propolis (A, B, C and D) concentrations. The ruminal liquid was anaerobically incubated at 39°C for 6 days in medium containing LLOS. After isolation, the strains were submitted to Gram staining and the bacterial growth was monitored by photospectrometer. It was evaluated the strain growth in the presence of the following subtracts: arabinose, cellulose, glucose, cellobiose, xylose, fructose, and lactose. In roughage diets, strains tolerant to LLOSC1 and LLOSB3 were similar to carbohydrates degradation, except lactose in which LLOSC1 was superior to strains tolerant to LLOSB3. For diets with 50:50 roughage:concentrate ratio, the products LLOSC3 and LLOSA2 stood out because they selected the highest number of strains able to degrade most of the tested carbohydrates. The results suggest that tolerance to propolis is higher in Gram-positive strains with several growth metabolic levels.

Tannin-tolerant bacteria from crossbred Holstein x Zebu cows

“…After elution with 50% acetone, two extractions with ethyl acetate using Rotavapor (Büchi Company, Flawil, Switzerland), and lyophilization (Freeze Dry System, Labconco Corporation, Kansas City, Missouri, USA), the purified condensed tannin powders were stored at 4 o C in the dark. Rumen contents were collected via cannulae, passed through sterile cheesecloth into sterile pre-warmed (40°C) thermos flasks, and taken in styrofoam boxes to the laboratory for bacterial isolation (Prado et al, 2010).…”

<b>Propolis extract and sodium monensin on ruminal fermentation and hematological parameters in sheep

“…Currently, consumers search for safe foods, derived from animals, which have not been fed antibiotics or growth promoters. Considering this situation, according to Ghisalberti (1979), propolis is a safe alternative given its pharmacological properties, including its antimicrobial, antimycotic and antiprotozoal activities (Prado et al, 2010b).…”

Propolis or cashew and castor oils effects on composition of Longissimus muscle of crossbred bulls finished in feedlot

“…When propolis is administered to animals its action is similar to ionophores, as observed through in vivo and in vitro experiments (Prado et al, 2010a). The potent bacteriostatic and bactericidal effects of propolis are the result of the combined actions of several such components.…”