Genetic monitoring of a Santa Ines herd using microsatellite markers near or linked to the sheep MHC

Petroli, César; Paiva, Samuel Rezende; Correa, Marilma Pachêco Chediak; McManus, Concepta

doi:10.1590/s1516-35982009000400012

Cited by 4 publications

(2 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A pilot study was carried out using the same 19 microsatellite loci as above in 191 animals of the conservation nucleus of Embrapa Tabuleiros Costeiros, Sergipe (Paiva et al, 2005b). Petroli et al (2009) using thirteen microsatellite loci on chromosome 20 (where the Sheep Major Histocompatibility Complex is found) identified a reduction in heterozygosity over the years in a control herd compared to the whole population. This may be due to the repeated use of the same sires in this herd.…”

Section: Genetic Management Of Herdsmentioning

confidence: 99%

Genetics and breeding of sheep in Brazil

2010

Self Cite

View full text Add to dashboard Cite

Studies in genetics and breeding of sheep in Brazil have increased significantly in recent years. These involve research in characterization, breeding and crossing sheep using new technologies available incorporating both classical quantitative and molecular genetics. Improvements in statistical techniques, computational resources as well as analysis of DNA and gaps in present knowledge and opportunities for possible research are pointed out. There is a need for greater interaction between various groups working in the country as well as interactions with other disciplines such as Geographical Information Systems, Statistics, Bioinformatics, as well as biological studies such as physiology and proteomics.

show abstract

Section: Genetic Management Of Herdsmentioning

confidence: 99%

Genetics and breeding of sheep in Brazil

2010

Self Cite

View full text Add to dashboard Cite

show abstract

“…A recent study of MHC gene-associated microsatellite loci including those of bovine, ovine, and goat breeds revealed convergent evolution, which was interpreted to be the result of similar selective pressures, acting in these different ruminant species [ 9 ]. Microsatellite loci associated with genes of the MHC complex can provide highly polymorphic molecular markers potentially associated with resistance against disease and can therefore be useful for conservation and breeding programs [ 19 , 28 , 31 ].…”

Section: Introductionmentioning

confidence: 99%

Genetic variability of six indigenous goat breeds using major histocompatibility complex-associated microsatellite markers

et al. 2011

View full text Add to dashboard Cite

The present study aimed at analyzing the genetic variability of indigenous goat breeds (Capra hircus) using the MHC-associated microsatellite markers BF1, BM1818, BM1258, DYMS1, and SMHCC1. The following breeds were included: Chinese Xuhuai, Indian Changthangi and Pashmina, Kenyan Small East African (SEA) and Galla, and Albanian Vendi. To examine genetic variability, the levels of heterozigosity, degrees of inbreeding, and genetic differences among the breeds were analyzed. The mean number of alleles ranged from nine in the Galla to 14.5 in the Vendi breed. The mean observed heterozygosity and mean expected heterozygosity varied from 0.483 in the Vendi to 0.577 in the Galla breed, and from 0.767 in the SEA to 0.879 in the Vendi breed, respectively. Significant loss of heterozygosity (p < 0.01) indicated that these loci were not in Hardy-Weinberg equilibrium. The mean FIS values ranged from 0.3299 in the SEA to 0.4605 in the Vendi breed with a mean value of 0.3623 in all breeds (p < 0.001). Analysis of molecular variance indicated that 7.14% and 4.74% genetic variation existed among the different breeds and geographic groups, whereas 92.86% and 95.26% existed in the breeds and the geographic groups, respectively (p < 0.001). The microsatellite marker analysis disclosed a high degree of genetic polymorphism. Loss of heterozygosity could be due to genetic drift and endogamy. The genetic variation among populations and geographic groups does not indicate a correlation of genetic differences with geographic distance.

show abstract

A comprehensive mapping of the structure and gene organisation in the sheep MHC class I region

et al. 2015

View full text Add to dashboard Cite

BackgroundThe major histocompatibility complex (MHC) is a chromosomal region that regulates immune responsiveness in vertebrates. This region is one of the most important for disease resistance because it has been associated with resistance or susceptibility to a wide variety of diseases and because the MHC often accounts for more of the variance than other loci. Selective breeding for disease resistance is becoming increasingly common in livestock industries, and it is important to determine how this will influence MHC polymorphism and resistance to diseases that are not targeted for selection. However, in sheep the order and sequence of the protein coding genes is controversial. Yet this information is needed to determine precisely how the MHC influences resistance and susceptibility to disease.MethodsCHORI bacterial artificial chromosomes (BACs) known to contain sequences from the sheep MHC class I region were sub-cloned, and the clones partially sequenced. The resulting sequences were analysed and re-assembled to identify gene content and organisation within each BAC. The low resolution MHC class I physical map was then compared to the cattle reference genome, the Chinese Merino sheep MHC map published by Gao, et al. (2010) and the recently available sheep reference genome.ResultsImmune related class I genes are clustered into 3 blocks; beta, kappa and a novel block not previously identified in other organisms. The revised map is more similar to Bovidae maps than the previous sheep maps and also includes several genes previously not annotated in the Chinese Merino BAC assembly and others not currently annotated in the sheep reference chromosome 20. In particular, the organisation of nonclassical MHC class I genes is similar to that present in the cattle MHC. Sequence analysis and prediction of amino acid sequences of MHC class I classical and nonclassical genes was performed and it was observed that the map contained one classical and eight nonclassical genes together with three possible pseudogenes.ConclusionsThe comprehensive physical map of the sheep MHC class I region enhances our understanding of the genetic architecture of the class I MHC region in sheep and will facilitate future studies of MHC function.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-015-1992-4) contains supplementary material, which is available to authorized users.

show abstract

Genetic monitoring of a Santa Ines herd using microsatellite markers near or linked to the sheep MHC

Cited by 4 publications

References 33 publications

Genetics and breeding of sheep in Brazil

Genetics and breeding of sheep in Brazil

Genetic variability of six indigenous goat breeds using major histocompatibility complex-associated microsatellite markers

A comprehensive mapping of the structure and gene organisation in the sheep MHC class I region

Contact Info

Product

Resources

About