Utilização de microssatélites e RAPD na caracterização molecular de acessos de Paspalum urvillei Steudel

Sawasato, Joaquim Taizo; Dall'Agnol, Miguel; Conceição, Daniele Priscila da; Tafernaberri, Vilmar; Klafke, Gabriel Baracy

doi:10.1590/s1516-35982008000800005

Cited by 8 publications

(7 citation statements)

References 11 publications

(14 reference statements)

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“…Although morphological analysis allows access to fewer characters, it represents effective variations in relation to the study interest agronomic characteristics, and is often more effective in comparison to plant breeding programs. Genetic variability assessment using microsatellite markers is considered an excellent tool in breeding programs, and is used in several other species, such as Paspalum urvillei, P. vaginatum, Zea mays, Oryza Sativa, Sorghum bicolor, among others (Wang et al, 2006, Sawasato et al, 2008. Molecular techniques combined with agronomic and morphological information contributes to breeding programs efficiency (Pereira et al, 2012, Cidade et al, 2013.…”

Section: Resultsmentioning

confidence: 99%

“…Information on genetic variability, seeking promising combinations, can be estimated through predictive methods from agronomic, morphological and molecular characteristics (Cavers et al, 2005, Cidade et al, 2013. Therefore, several characters simultaneous evaluation can be an efficient tool to characterize populations, identify superior genotypes and assist in directing plant breeding future crosses (Sawasato et al, 2008). Thus, the knowledge on genetic variability on expression of characters among Paspalum genus genotypes, analyzed in field and DNA levels, may provide subsidies for superior plants selection and new hybridization targeting with sex parents, in search of elite recombinants.…”

Section: Introductionmentioning

confidence: 99%

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Agronomic performance and interspecific hybrids selection of the genus Paspalum

Pereira¹,

Dall'Agnol

Simioni

et al. 2015

Científica

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The objective of the study was to analyze the genetic variability at agronomic and molecular level of apomictic genotypes of the genus Paspalum obtained by interspecific crosses of Paspalum plicatulum (sexual famale genitor) and Paspalum guenoarum (apomictic male genitor) to predict promising combinations with the sexual genitor using characters related to forage production. The study was conducted in two environments in 2010 and 2011 in Eldorado do Sul and Augusto Pestana, State of Rio Grande do Sul, Brazil. The experiment followed a randomized block design with four replicates at both locations. Seven interspecific hybrids, the two genitors (sexual "4c-4x" P. plicatulum and apomictic cultivate "Rojas" P. guenoarum) and two native genotypes of Paspalum guenoarum and cultivate Pensacola of P. notatum were used. Analysis done inagronomic and molecular levels were able to detect genetic variability among interspecific hybrids of Paspalum. Moreover, the results suggested that the hybrids H12, H13 and H20 are recommended for further hybridizations with female sexual parent obtaining elite recombinant.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Agronomic performance and interspecific hybrids selection of the genus Paspalum

Pereira¹,

Dall'Agnol

Simioni

et al. 2015

Científica

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“…Our phenotypic data allowed the detection of two structural groups. Sawasato et al (2008), using 7 SSR primers in a panel of 64 Brazilian accessions of P. urvillei, detected seven structure groups. This difference in the number of groups may be due to these authors using primers from Zea mays L., Sorghum bicolor, Trifolium repens, and Lolium multiflorum L. In addition, the limited number of primers generated 28 polymorphic bands.…”

Section: Discussionmentioning

confidence: 99%

Understanding the extent of phenotypic variability in accessions of Paspalum urvillei Steud. from the USDA NPGS

Ferreira¹,

Gwinner²,

Ferreira³

et al. 2020

Iheringia Ser Bot

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The objective of this study was to investigate the phenotypic variability in Paspalum urvillei Steud. related to twelve zootechnical traits to better understand the diversity of 41 accessions from the USDA GRIN platform from seven countries. First, we performed a structure inference based on the phenotypic data, identifying two structure groups, and the majority of Brazilian accessions belonged to the first group. Then, we performed principal component analysis and principal coordinate analysis, making Violin plots for each trait within each structure group. Next, we performed spatial clustering of the accessions and utilized an unweighted pair group method with arithmetic mean clustering. In brief, the Brazilian P. urvillei breeding programs may be successful by importing USDA germplasm resources from the second phenotypic structure group.Keywords: cluster analysis, germplasm bank, forage RESUMO -Entendendo a extensão da variabilidade fenotípica em acessos de Paspalum urvillei Steud. do banco de germoplasma do USDA NPGS. O objetivo deste estudo foi investigar a variabilidade fenotípica de Paspalum urvillei em relação a doze características zootécnicas, a fim de compreender melhor a diversidade de 41 acessos da plataforma USDA GRIN de sete países. Primeiro foi realizada uma inferência de estrutura baseada nos dados fenotípicos, identificando dois grupos estruturados, onde a maioria dos acessos brasileiros é inferida ao primeiro grupo. Em seguida, examinou-se a análise de componentes principais e análise de coordenadas principais, foram feitos gráficos de violino para cada característica dentro de cada grupo estruturado. Por fim, foi realizado o agrupamento espacial dos acessos e o método de médias não ponderadas de grupos pareados. Em suma, o programa brasileiro de melhoramento de P. urvillei pode alcançar sucesso importando acessos do USDA inferidos ao segundo grupo estruturado.Palavras-chave: análise de agrupamento, banco de germoplasma, forragem

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“…A total of 11 primers were tested based on studies performed with Lolium multiflorum L. [31], Paspalum vaginatum Sw. [30], Trifolium repens L. [32] and Paspalum urvillei St. [33] (Table 1). Amplified fragments were electrophoresed in 4% agarose gels containing 0.08 μL/mL ethidium bromide (10 mg/mL) and visualized on an ultraviolet light transilluminator (wavelength 260 nm).…”

Section: Methodsmentioning

confidence: 99%

Genetic Similarity Among Accessions of Paspalum notatum Flügge (Poaceae): a Potential to Parental Selection

Fachinetto

Dall'Agnol

Schneider-Canny

et al. 2021

Braz. arch. biol. technol.

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Paspalum notatum is an important forage grass contributing significantly to the coverage of the natural fields of Southern Brazil. Simple sequence repeat (SSR) markers were used to evaluate the genetic similarity of strains within a P. notatum collection. Genomic DNA was extracted in bulk from young leaves of five plants from each accession obtained from the USDA. In the molecular analysis, the eight SSR markers evaluated formed seven distinct groups, and two isolated genotypes, with an average similarity index of 0.29, ranging from zero to 0.83. All the loci were polymorphic and the polymorphism information content ranging from 0.41 to 0.69. The results evidenced a low genetic similarity, which can be explored via parental selection in a breeding program.

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Utilização de microssatélites e RAPD na caracterização molecular de acessos de Paspalum urvillei Steudel

Abstract: RESUMO -Este estudo foi realizado com o objetivo de verificar a diversidade genética na coleção de acessos de P. urvillei

Cited by 8 publications

References 11 publications

Agronomic performance and interspecific hybrids selection of the genus Paspalum

Agronomic performance and interspecific hybrids selection of the genus Paspalum

Understanding the extent of phenotypic variability in accessions of Paspalum urvillei Steud. from the USDA NPGS

Genetic Similarity Among Accessions of Paspalum notatum Flügge (Poaceae): a Potential to Parental Selection

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