Deposição de ácido linoléico conjugado (CLA) em tilápias-do-nilo

Abstract

RESUMO -Objetivou-se avaliar a deposição de ácido linoléico conjugado (CLA) em filés de tilápia-do-nilo (Oreochromis niloticus). Foram utilizados peixes com 121,08 ± 8,48 g, distribuídos em quatro caixas de cimento amianto (1,0 m 3 cada uma) e arraçoados até saciedade aparente, duas vezes ao dia, durante 49 dias. Como alimento, utilizou-se ração comercial extrusada com 28% de PB e 3.000 kcal de energia digestível/kg, acrescida de 2% do produto comercial Luta-CLA ® (BASF -Brasil). No início do experimento, cinco peixes foram coletados aleatoriamente como amostra controle (sem CLA). A cada sete dias, dois peixes de cada caixa foram coletados, pesados e filetados para determinação da taxa de deposição de CLA nos filés. As amostras de filés foram estocadas a -24 o C para análise do teor de lipídios totais e do perfil de ácidos graxos. A taxa de deposição de CLA foi determinada pela função de Gompertz (y = a . exp{-b . exp[-kt]}). O meio-tempo (T) para deposição de 50% do CLA foi estimado pela equação T = ln2/k, em que: T representa o meio-tempo; ln , o logaritmo Neperiano e k, a unidade de tempo que fornece a "velocidade" de deposição do CLA no tecido muscular da tilápia-do-nilo. A deposição máxima de CLA nos lipídios totais do filé de tilápias-do-nilo estimada pela função de Gompertz foi de 1,61 g/100 g de lipídios do filé. O meio-tempo de deposição de CLA nos lipídios do filé ocorre com 6,86 dias. Palavras-chave: ácidos graxos, ácido linoléico conjugado, Oreochromis niloticus Deposition of conjugated linoleic acid (CLA) in Nile tilapiaABSTRACT -This study was carried out to determine the deposition of conjugated linoleic acid (CLA) in Nile tilapia Oreochromis niloticus fillet. Fish averaging 121.08 ± 8.48 g were distributed in four cement amianthus tank (1.0 m 3 each) and hand fed to apparent satiation, two times a day for 49 days. As feed, an extruded commercial diet with 28% CP and 3000 kcal of digestible energy was utilized. A commercial CLA product (Luta-CLA ® , BASF) was added at 2% of the diet. At the beginning of the experiment, five fish were randomly collected as a control sample (without CLA). Weekly, two fish of each tank were collected, weighed and filleted for measurement of CLA deposition ratio in the fillets. The fillet samples were stored at -24 o C for total lipid and analyses of fatty acids profiles. The deposition rate of CLA was determined by Gompertz function (y = a . exp{-b . exp[-kt]}). Half time (T) to have 50% deposition of CLA was calculated by the equation T = ln2/k, where T is half time; ln is Napierian logarithm and constant k the time unit, which provides the "velocity" in the deposition of CLA in the Nile tilapia fillets. The maximum CLA deposition in the fish fillet lipids, estimated by the Gompertz function, was 1.61 g/100 g fillets lipids. Half time of CLA deposition in the fillet lipids occur within 6.86 days.Key Words: conjugated linoleic acid, fatty acids, Oreochromis niloticus IntroduçãoEntre os setores da produção animal, a aqüicultura é o que se expande mais rapidamente. A t...

Incorporation of conjugated linoleic acid (CLA) and α-linolenic acid (LNA) in pacu fillets

“…The DHA concentrations in the fish fillets found at the end of the experiment were lower (P<0.05) in all diets in relation to the value determined initially (Table 6), different from the results of Zanqui et al (2013) and Santos et al (2007a), who did not observe reduction in the DHA levels in fish fillets fed diets containing CLA.…”

Manipulation of the fatty acids composition of poultry meat and giblets by dietary inclusion of two oil sources and conjugated linoleic acid

“…Addition of CLA in the diet resulted in an increase (P<0.05) in CLA deposition in analyzed tissues (Table 3), which agrees with previous studies (Badinga et al, 2003;Kim et al, 2007;Santos et al, 2007). The highest value for deposition of CLA was detected in the heart tissue and the lowest value for CLA was observed in breast meat (Table 3).…”

Dietary conjugated linoleic acid (CLA) for finishing Nile tilapia

“…The level of CLA in dairy products and meat of ruminants is very low, between 3 and 6 mg g −1 of lipids, so we need to increase the presence of CLA on other products, to be able to achieve the desired amount of CLA consumption. Temporal dietary CLA deposition in fish muscle (Santos et al. 2007a; Ramos et al.…”

“…2007b). More recently, Santos et al. (2007a) demonstrated that Nile tilapia can deposit more than 26 mg g −1 of CLA in the lipids.…”