Immunohistochemical study of basement membrane collagen IV in uterine cervix carcinoma

Abstract

We conclude that immunostaining for collagen IV may contribute to the diagnosis of stromal invasion by BM discontinuity.

Immunohistochemical evaluation of type IV collagen and laminin-332 γ2 chain expression in well-differentiated oral squamous cell carcinoma and oral verrucous carcinoma: a new recommended cut-off

“…The type IV collagen immunostaining pattern of all groups (A, B and C) have been summarized in Table 2 and histopathological findings of these groups have been shown in Figs 1–3. In this study the excluded cases showed negative type IV collagen immunostaining in internal controls, even after repeating the technique or using of alternative protocols (10, 15). Statistically, significant differences were observed in type IV collagen staining pattern among three groups ( P = 0.000).…”

“…Therefore, loss of BM may be associated with stromal invasion and metastatic process (9, 10, 15). For these reasons, there is an interest in the use of immunohistochemical study of BM components as an adjunct to routine light microscopy (10, 15). In the present study the staining pattern was more frequently continuous (72.72%) in epithelial hyperplasia with no dysplasia (group A).…”

“…However, we could not find significant differences in BM staining pattern between OVC and well‐differentiated OSCC. Discontinuity of BM type IV collagen has been reported in some extra oral carcinomas such as uterine cervix carcinoma, invasive vulvar carcinoma, invasive cervical carcinoma, and cystadenocarcinoma of ovary (15, 18, 19). Also, some previous studies in OSCC had same results with our study (11, 20, 21).…”

“… Neoplastic cells would secrete BM degrading enzymes such as type IV collagenase and plasminogen that play a key role in loss of BM continuity (7, 10, 15). Therefore, in our study it seems, neoplastic cells of OVC may have the ability to degrading enzyme secretion where as, these cells may not have migration potential which is important for invasion. Adjacent normal cells may secrete degrading enzymes after stimulation by tumor cells (15). Decrease or discrepancy in synthesis of BM components by neoplastic cells (7, 10, 15).…”

“…Therefore, in our study it seems, neoplastic cells of OVC may have the ability to degrading enzyme secretion where as, these cells may not have migration potential which is important for invasion. Adjacent normal cells may secrete degrading enzymes after stimulation by tumor cells (15). Decrease or discrepancy in synthesis of BM components by neoplastic cells (7, 10, 15). Decrease in capacity of neoplastic cells for repairing of BM defects produced by inflammatory cells (8, 15).…”