Effect of salt leaching on PCL and PLGA(50/50) resorbable scaffolds

Abstract

The use of porous bioresorbable scaffolds in the field of tissue engineering represents an alternative for the treatment of lesions and losses of biological tissues. This work evaluates the leaching salt effect of two different processes and polymers. Dense and porous scaffolds were prepared with poly(epson-caprolactone) (PCL) and poly(D,L-lactic acid-co-glycolic acid) (50/50) (PLGA50) by casting and melting compression process. Sodium citrate with particles sizes of 180-250 µm of diameter was used as porogen. The dense and porous samples were immersed in distilled water for 30 hours and evaluated for pH and mass variations, by scanning electronic microscopy (SEM), differential scanning calorimetric (DSC) and thermogravimetric analysis (TGA). The results of the analyses showed that the inclusion of the salt and leaching process did not affect the properties of the scaffold, indicating that the method is useful to make porous scaffolds to be potentially used in tissue engineering.

Analysis of the growth pattern of Vero cells cultured on dense and porous poly (L-Lactic Acid) scaffolds

“…Numerous materials can be used as scaffolds, but temporary scaffolds are of special interest since support should be present during tissue repair in order to promote guided tissue growth. Among the various characteristics of bioresorbable materials, porous morphology is important because the presence of pores permits cell penetration and tissue ingrowth 9 . The objective of the present study was to evaluate how variations in the pore diameter of PLLA scaffolds changes the growth and proliferation pattern of cells cultured on these scaffolds.…”

Repurposing biodegradable tissue engineering scaffolds for localized chemotherapeutic delivery

“…Traditionally, porous biodegradable polymeric sponges have primarily been utilized as scaffolds for tissue remodeling in various tissue engineering applications (Barbanti, Carvalho Zavaglia, & de Rezende Duek, ; Cho, Kim, You, & Cho, ; Huang, Zhu, Tu, & Wan, ; Murphy, Dennis, Kileny, & Mooney, ; Santamaría et al, ; Yang, Chen, Shen, & Tan, ). The premise is that if cells are seeded in the porous scaffold or sponge along with the appropriate growth factors, that they will be able to proliferate and remodel the damaged tissue as the scaffold degrades.…”

“…To fabricate porous biodegradable tissue engineering scaffolds, two techniques are typically employed, solvent‐quenching and salt‐leaching (Barbanti et al, ; Cho et al, ; Huang et al, ; Loh & Choong, ; Murphy et al, ; Yang et al, ). For solvent‐quenching, the polymer is first dissolved in a solvent with a relatively high freezing point (e.g., 1,4‐dioxane) and the polymer/solvent solution is immediately frozen at different temperatures (e.g., −20°C, −80°C, or in liquid nitrogen) prior to lyophilization.…”

In vitro and in vivo studies of rhBMP2‐coated PS/PCL fibrous scaffolds for bone regeneration

“…There are plenty of methods to fabricate porous scaffolds for bone tissue engineering such as 3D printing,4 hydrogel,7 salt leaching,8 etc. Among these methods, electrospinning is the simplest method that can easily create a porous scaffold by random or aligned arrangement of nano‐or micro‐fibers.…”