Multiplicação in vitro de segmentos apicais caulinares de segurelha (Satureja hortensis L.)

Abstract

RESUMO:O presente estudo teve por objetivo avaliar o efeito de BAP, na presença e ausência de ANA, sobre a multiplicação in vitro de segmentos apicais caulinares de Satureja hortensis. Os explantes foram isolados de plântulas germinadas in vitro e cultivados em meio nutritivo MS. O delineamento experimental utilizado foi o inteiramente casualizado, em esquema fatorial 2x5, correspondendo às concentrações de ANA (0 e 1 µM) e de BAP (0; 5; 10; 15 e 20 µM), com seis repetições, cada uma composta por três explantes. Para a porcentagem de explantes com brotações houve efeito significativo para o fator BAP, aumentando à medida que cresce a concentração da citocinina. A variável "número de brotos por explante" apresentou interação entre os fatores, havendo a maior formação de brotos na presença de ANA na faixa de 10 -15 µM de BAP. Na presença da auxina, o maior valor ocorreu com 15 µM de BAP tendendo a diminuir independente da presença de ANA. O enraizamento dos segmentos apicais foi maior na presença de ANA e ausência de BAP, diminuindo com o aumento da citocinina. O número de folhas foi influenciado pela maior concentração de BAP sendo que a 15 µM foi observado o maior número, tendendo à queda com aumento da concentração. A concentração de 15 µM de BAP, independente de ANA, proporcionou os melhores resultados na multiplicação da espécie produzindo aumento de brotações e número de folhas, à exceção do enraizamento que foi influenciado pela auxina.Palavras-chave: cultura de tecidos, regulador de crescimento, plantas medicinais, Lamiaceae. ABSTRACT:In vitro multiplication of apical shoot segments of Summer Savory(Satureja hortensis L.).This study aimed to evaluate the effect of BAP in the presence and absence of NAA, on the in vitro multiplication of shoot apical segments of Satureja hortensis. The explants were isolated from seedlings germinated in vitro and cultured in nutrient medium MS. The experimental design was completely randomized in a 2x5 factorial arrangement, corresponding to the concentrations of NAA (0 and 1 µM) and BAP (0, 5, 10, 15 and 20 µM), with six replicates, each consisting of three explants. For the percentage of explants with shoots, there was no significant effect for the factor BAP, increasing as the concentration of BAP grows. The variable number of shoots per explant showed interaction between factors, with the highest shoot formation in the presence of NAA and BAP in the range of 10 -15 µM. In the presence of auxin, the highest value occurred with 15 µM BAP, tending to decline regardless of the presence of NAA. The rooting of the apical segments was higher in the presence of NAA and absence of BAP, decreasing with increasing cytokinin. Leaf number was influenced by the higher concentration of BAP, and the amount of 15 µMhadthe largest number, tending to decrease with increasing concentration. The concentration of 15 µM BAP, regardless of NAA, provided better results in the multiplication of the species, producing increased number of shoots and leaves, except for rooting, whichwas influenced ...

Shoot multiplication of two <em>Sequoia sempervirens </em> genotypes with addition of small concentrations of kinetin

“…It was noteworthy that increasing the concentration of NAA had an inhibitory role in the production of shoots on both genotypes (A228 and A140). This was also observed from a study with the medicinal plant Satureja hortensis L., in which increasing concentrations of NAA diminished the number of shoots (Navroski et al, 2014). On the contrary, NAA stimulated the emission of shoots by an interaction with BAP in clones of Eucalyptus globulus Labill.…”

In vitro multiplication of wild Manihot species with different naphthaleneacetic acid and benzylaminopurine concentrations

“…The highest NR value (7.54) was observed in plants treated with 0.025 mg L -1 NAA, in the absence of BAP (Table 6). A similar response was observed during the in vitro multiplication of Satureja hortensis L. stem segments (Navroski et al, 2014) in cultivation medium containing a low concentration of NAA and no BAP. The effect on NR provided evidence of the role of auxin in root development.…”

Lamiaceae Essential Oils, Phytochemical Profile, Antioxidant, and Biological Activities

“…Research on the genus Satureja L. reports that it is distributed in 30 species around the globe, and that it has beneficial properties for human health, such as in the treatment of pain caused by oxidative stress. Therefore, the essential oils from leaves and stems of Satureja spicigera L , Satureja cuneifólia L, and Satureja hortensis L. have compounds that work as antioxidants [ 82 , 83 ].…”