Estabelecimento in vitro e micropropagação de maracujá silvestre (Passiflora foetida L.)

Soares, W.S.; Rêgo, Maílson Monteiro do; Rêgo, Elizanilda Ramalho do; Barroso, Priscila Alves; Nascimento, Karinne Siva do; Ferreira, K. T. C.

doi:10.1590/s1516-05722012000500002

Cited by 10 publications

(7 citation statements)

References 11 publications

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“…glaziovii do not follow this triphasic pattern and show radicle protrusion after 120 h of soaking in water. This difficulty during germination was previously observed by Soares et al (2012), who reported that P. foetida L. seeds might require months to germinate, most likely due to dormancy.…”

Section: Discussionsupporting

confidence: 63%

Morphometry, in vitro-ex vitro germination and tetrazolium testing of stinking passionflower [Passiflora foetida var. glaziovii Killip] (Passifloraceae) seeds

Costa¹,

Oliveira²,

Araújo³

et al. 2016

Aust J Crop Sci

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Passiflora foetida L. has broad utility as a medicinal and ornamental plant; however, information regarding varieties and the physiological potential of their seeds are scarce. Here, we aim to evaluate in vitro and ex vitro germination behaviour and to adapt tetrazolium methodology for P. foetida var. glaziovii Killip. The botanical identification and characterisation were performed through the observation of morphological characters and consultation of the relevant literature. Ex vitro germination was performed on rolled and flat Germitest paper at continuous (25 °C) and alternating (20 and 30 °C; night and day, respectively) temperatures. Seeds with the completely removed integument were used for in vitro germination and tetrazolium tests. The in vitro germination test was performed with MS medium using seeds conditioned under different light qualities (white, red, far-red or darkness) and at two temperature regimes (25 °C; 20 and 30 °C alternating temperature). For the tetrazolium test, seeds were soaked in different concentrations of tetrazolium salt (2.5, 5.0, 7.5 or 10 g L -1 ) at different temperatures (30, 35, 40 or 45 °C) for two hours in the dark. This is the first record of P. foetida var. glaziovii, a wild herbaceous vine with glandular trichomes on its leaves, bracts and stipules; fruit bacoid, elliptic, glabrous, in Espírito Santo State, Brazil. The seeds are small (approximately 4.87 mm long and 2.15 mm wide) and non-photoblastic, and in vitro germination must be performed using an alternating temperature (20 and 30 °C) regime. The best result of germination was achieved using a concentration-temperature combination of 10 g L -1 tetrazolium and 30 °C.

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Section: Discussionsupporting

confidence: 63%

Morphometry, in vitro-ex vitro germination and tetrazolium testing of stinking passionflower [Passiflora foetida var. glaziovii Killip] (Passifloraceae) seeds

Costa¹,

Oliveira²,

Araújo³

et al. 2016

Aust J Crop Sci

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“…Becerra et al (2004) compared juvenile plants P. edulis explants to explants from adult plants revitalized by extreme pruning and plants cultured in vitro and concluded that the age of the plants was the most important factor influencing the morphogenic response. For this reason, is generally prefered to obtain explants from seeds germinated in vitro when using different species of Passiflora (Rêgo et al, 2011;Carvalho et al, 2012;Soares et al, 2012).…”

Section: Resultsmentioning

confidence: 99%

In vitro culture of shoot apices from juvenile and adult yellow passion fruit plants

Junghans

Andrade

Simões

et al. 2014

Agraria

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The aim of the present study was to evaluate in vitro plant regeneration from the shoot apices of juvenile and adult passion fruit plants and provide a basis for future clonal cleanup of superior genotypes through the use of tissue culture. The studies were divided into three experiments to evaluate the effect of age and origin of the donor plant, the culture medium methodology and the size of the explants. The shoot apices were inoculated in Murashige & Skoog (MS) medium with 3% sucrose and three 6-benzylaminopurine (BAP) concentrations: 0, 1 or 2 mg L -1 . Next, the samples were transferred to a half-strength mineral salt medium (½ MS) and supplemented with 1 mg L -1 indolebutyric acid (IBA). Two cultivation methods were used: one with variations in the cultivation period in the MS medium (26 or 60 days) and the other with or without transfer to half-strength mineral salt medium (½ MS). The methodology by which explants are maintained in MS medium supplemented with 1 mg L -1 BAP for 26 days, followed by transfer to ½ MS medium supplemented with 1 mg L -1 IBA for 21 days of cultivation and then transfer to ½ MS medium for 14 days is more appropriate for regenerating plants from the shoot apices of adult Passiflora edulis (AGBP accession 38).Key words: growth regulators, Passiflora edulis, tissue culture Cultivo in vitro de ápices caulinares provenientes de plantas jovens e adultas de maracujazeiro amarelo RESUMO O objetivo deste trabalho foi avaliar a regeneração de plantas in vitro a partir de ápices caulinares provenientes de maracujazeiros jovens e adultos e fornecer subsídios para futuros trabalhos de limpeza clonal de genótipos superiores, via cultura de tecidos. Os estudos foram divididos em três experimentos visando avaliar o efeito da idade e a procedência da planta doadora, da metodologia do meio de cultivo e do tamanho dos explantes. Os ápices caulinares foram inoculados em meio de estabelecimento Murashige & Skoog (MS), acrescido de 3% de sacarose e com três concentrações de BAP: 0; 1 mg L -1 ou 2 mg L -1 . Em seguida, foram transferidos para o meio com metade das concentrações dos sais minerais (½MS) acrescida de 1 mg L -1 de AIB. Duas formas de cultivo foram utilizadas variando o período de cultivo no meio de estabelecimento (60 dias ou 26 dias) e a transferência ou não para o meio, com metade das concentrações dos sais minerais (½MS). A metodologia na qual os explantes são mantidos em meio MS acrescido de 1 mg L -1 de BAP durante 26 dias seguidos de transferência para o meio ½MS acrescido de 1 mg L -1 de AIB, por 21 dias de cultivo e, posteriormente, para o meio ½MS, por 14 dias, é mais apropriada para regenerar plantas a partir de ápices caulinares de plantas adultas de Passiflora edulis (acesso BGP 38).Palavras-chave: reguladores de crescimento, Passiflora edulis, cultura de tecidos

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“…at the intermediate callus phase, instability may occur in the genotype of the obtained plantlets, becoming an obstacle to large-scale propagation. Furthermore, less organogenic calli formation favours the largescale production of plantlets, in view of the greater somaclonal stability of plant material grown in vitro (SOARES et al, 2012).…”

Section: Figures 4a and 4b Show The Percentage Values For The Formation Of Organogenic Calli (Foc)mentioning

confidence: 99%

The in vitro multiplication of anthurium cv. Eidibel

Lima

Campos

Carvalho

2020

AGRO@MBIENTE ON-LINE

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Anthurium is one of the most marketed species in international floriculture. The availability of sufficient plants of phytosanitary quality is a limiting factor in their commercial cultivation. Micropropagation is the most common technique used in producing plantlets on a large scale. The aim of this study was to determine the most suitable relationship between growth medium, BAP concentration and photoperiod for increasing the in vitro multiplication rate of the ‘Eidibel’ cultivar. A completely randomised design was used in a 2 x 9 x 2 factorial scheme with 20 replications. The treatments comprised combinations of two types of growth medium (MS and Pierik), nine concentrations of BAP (0.0; 1.11; 2.22; 3.33; 4.44; 5.55; 6.66; 7.77 and 8.88 μM), and two photoperiods (12 and 16 h). The number of shoots per explant (NSE) and percentage of explants forming organogenic calli (FOC) and roots (FR) were evaluated after 60 days. The highest values for NSE, 3.66 and 4.69, were obtained in the MS and Pierik growth media, containing 8.88 μM and 4.85 μM BAP respectively. The NSE was higher under the 12-hour regime in the MS medium, and equal under the two photoperiods in the Pierik medium. The lower the BAP concentration, the greater the percentage of root formation and the smaller the percentage of organogenic calli. The Pierik medium is recommended for the production of micropropagated ‘Eidibel’ plantlets, with the addition of 4.85 μM BAP, under a photoperiod of 12 h.

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Estabelecimento in vitro e micropropagação de maracujá silvestre (Passiflora foetida L.)

Cited by 10 publications

References 11 publications

Morphometry, in vitro-ex vitro germination and tetrazolium testing of stinking passionflower [Passiflora foetida var. glaziovii Killip] (Passifloraceae) seeds

Morphometry, in vitro-ex vitro germination and tetrazolium testing of stinking passionflower [Passiflora foetida var. glaziovii Killip] (Passifloraceae) seeds

In vitro culture of shoot apices from juvenile and adult yellow passion fruit plants

The in vitro multiplication of anthurium cv. Eidibel

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