Perfil de suscetibilidade de leveduras do gênero Candida isoladas de animais ao óleo essencial de Rosmarinus officinalis L

Abstract

Neste trabalho foram avaliadas a atividade antifúngica e composição química do óleo essencial de Rosmarinus officinalis (alecrim). O óleo essencial foi obtido, através da hidrodestilação, das partes aéreas da planta, o qual foi analisado por cromatografia gasosa com detectores de ionização em chama (GC/FID) e espectrometria de massas (GC/MS). Esse óleo foi testado em isolados de C. albicans da mucosa vaginal de fêmeas caninas, casos clínicos de candidíase em animais cepas padrões e espécies não-albicans, usando a técnica de microdiluição em caldo (CLSI-M27A2). A análise cromatográfica do óleo permitiu identificar como principais compostos cânfora, verbenona e 1,8-cineol. Os valores de CIM do óleo para os isolados padrões de C. albicans variaram entre 1,25 e 2,5 µL mL-1 e de 2,5 a 5,0 µL mL-1 para CFM, enquanto que para os isolados não-albicans observou-se CIM entre 1,25 e 5,0 µL mL-1 e CFM de 2,5 a 10,0 µL mL-1. C. albicans isolada de animais apresentou valores de 2,5 a >10,0 µL mL-1 para CIM e de 5,0 a >10,0 µL mL-1 para CFM. Os resultados demonstraram atividade fungicida e fungistática do óleo essencial de alecrim em isolados de Candida spp. provenientes de animais.

Effects of essential oils of Rosmarinus officinalis Linn. and Origanum vulgare Linn. from different origins on Sporothrix brasiliensis and Sporothrix schenckii complex

“…activity of the extracted oil with the same methodology used in our study and described the MIC of 11mg/mL in an only S. schenckii strain tested, and the plant did not show fungicidal activity. The fungistatic activity found by Luqman et al (2007) was similar to our study, but our results showed the effect of MFC 50 and MFC 90 against overall tested isolates at ≤2.25mg/mL and 36mg/mL for extracted oil, respectively, and 9mg/mL and 36mg/mL for commercial oils, demonstrating the good fungicidal activity of the essential oil of R. officinalis L. Other pathogenic fungi have been highly susceptible to rosemary oil, as Candida albicans (Bozin et al, 2007;Cleff et al, 2012), that was inhibited and eliminated by the plant oil at 5.5mg/mL and 11mg/mL, respectively, as well as Crytococcus neoformans (Luqman et al, 2011). The dermatophytes Epidermophyton floccosum, Trichophyton mentagrophytes, T. rubrum and Microsporum canis were susceptible in the MIC of 15 to 30.2μL/mL (Bozin et al, 2007) and T. rubrum and M. gypseum were inhibited at 1.38mg/mL and 2.75mg/mL, respectively (Luqman et al, 2007).…”

Antimicrobial activity of fixed oils found in Brazil nuts and sunflower seeds against microorganisms isolated from bovine subclinical mastitis

“…Regarding isolated microorganisms of Candida spp., the oils under analysis also had inhibitory activity in low concentrations; this fact is relevant since Candida represents the most commonly isolated pathogen from infections found in mammary glands of dairy cows, among all yeasts (Santos and Marin, 2005;Wunder, 2007). Besides, these results are also important due to the development of resistance against azolic agents which may cure candidiasis (caused by this yeast) in animals (Cleff et al, 2012).…”

Essential Oils and Their Bioactive Compounds in the Control of Gastrointestinal Nematodes of Small Ruminants

“…Os OE apresentam diversas atividades farmacológicas de interesse na medicina veterinária, como ação antibacteriana [67,86], antifúngica [15,66], coccidicida [41,79], carrapaticida [12,74] e anti-helmíntica [23,54,82].…”