“…Different hormonal combinations and acclimatization conditions were optimized when leaves or foliar segments were used as explants. The combination of 1.3 mg l −1 of benzyl amino purine (BAP) and 0.5 mg l −1 of NAA was found as the most efficient combination for direct regeneration of plantlets, and during acclimatization, the substrate containing coconut powder, Biosafra® (12 g l −1 ), limestone (1 g l −1 ) and vermiculite (1:1) was supplemented with MS salts (Arrigoni-Blank et al 2011). Multiple shoots were obtained from mature leaf explants of Pelargonium rapaceum (L.) L' Hérit.…”