Efeito fungitóxico in vitro do óleo resina e do óleo essencial de copaíba (Copaifera multijuga Hayne)

Abstract

Federal do Pará. Av. Augusto Correa, s/n-Guamá. Belém, Pará, Brasil. RESUMO: Óleo de Copaifera multijuga Hayne, in natura e as frações foram avaliadas quanto às atividades fungitóxicas in vitro, frente a cinco espécies de fungos filamentosos do gênero Aspergillus e três espécies de leveduras do gênero Candida. Concentrações de óleo resina e de óleo essencial na faixa de 0,08 mg mL -1 a 1,6 mg mL -1 foram usadas para as análises qualitativa e quantitativas. As amostras foram dispostas sobre discos de papel de 5 mm de diâmetro e distribuídos sobre o meio Saboraud em placas de Petri, inoculadas com esporos dos microorganismos e incubadas a 28ºC durante 10 dias. Utilizou-se solução com 1,6 mg mL -1 de nitrato de miconazol como controle positivo. Os resultados qualitativos mostraram que o óleo resina apresentou boa atividade fungistática, porém uma das frações do óleo essencial se mostrou altamente efetiva contra Candida parapsilosis IOC-2882, Aspergillus flavus IOC-3874 e A tamarii IOC-187 com halos de inibição de 16,0±1,4 mm, 19,5±2,1 mm e 12,5±3,5 mm, respectivamente. Já a avaliação quantitativa mostrou que 0,3 mg mL -1 do óleo resina inibiu o crescimento de A. flavus e C. parapsilosis, enquanto que 0,08 mg mL -1 da fração do óleo essencial atingiu esta mesma atividade. Palavras-chave: Copaifera multijuga, óleo essencial, atividade antifúngico, Aspergillus, Candida ABTRACT:In vitro fungitoxic effect of the oil-resin and the essential oil of copaiba (Copaifera multijuga Hayne). Copaifera multijuga Hayne oil, crude and fractions, was evaluated for its in vitro fungitoxic activities against five species of filamentous fungi belonging to the genus Aspergillus and three yeast species of the genus Candida. Oil-resin and essential oil concentrations ranging from 0.008 mg mL -1 to 1.6 mg mL -1 were used in qualitative and quantitative analyses. Samples were placed onto 5mm-diameter paper disks and distributed on Saboraud culture medium in Petri dishes inoculated with conidia and incubated at 28ºC during 10 days. A solution with 1.6 mg mL -1 of miconazole nitrate was used as positive control. Qualitative results indicated that the oil-resin presented good fungistatic activity; however, one of the essential oil fractions was highly effective against Candida parapsilosis IOC-2882, Aspergillus flavus IOC-3874 and A. tamarii IOC-187, with inhibition halos of 16.0±1.4 mm, 19.5±2.1 mm and 12.5±3.5 mm, respectively. The quantitative evaluation indicated that 0.3 mg mL -1 of the oil-resin inhibited Aspergillus flavus and Candida parapsilosis growth, whereas 0.08 mg mL -1 of the essential oil fraction reached this same activity.

Chemistry and Biological Activities of Terpenoids from Copaiba (Copaifera spp.) Oleoresins

“…The fungicidal activity fungicida of C. multijuga oleoresin in natura , and a volatile fraction obtained of the hydrodistillation of this oleoresin, were evaluated in vitro against filamentous fungi ( Aspergillus ) and yeast ( Candida ). Samples were compared with the antibiotic drug Miconazole nitrate (MIC = 0.1–0.5 µg/mL), the volatile fraction being more active (MIC = 0.08–0.5 µg/mL) [ 41 , 42 ].…”

Observations on the therapeutic practices of riverine communities of the Unini River, AM, Brazil

“…Similar uses were described for copaiba in surveys conducted in the Amazon region by Branch and Silva (1983), Amorozo and Gé ly (1988), Balé e (1994), Pinto and Maduro (2003), Shanley and Rosa (2005) and Rodrigues (2006). Some studies have evaluated the anti-inflammatory effects of its essential oil (Kobayashi et al, 2011), the antifungal effect of its essential oil and resin (Deus et al, 2009) and the antiinflammatory and antinociceptive activity of the oleoresin (Gomes et al, 2010). The use of the bark from the paracanauba and uxi-liso plants for the treatment of genitourinary problems was described in ethnopharmacological studies performed in the Amazon region by Branch and Silva (1983) and by us, Rodrigues (2006), while studies on the pharmacological action and contraceptive properties of these two species were not found in the literature.…”

Avaliação alelopática de extratos etanólicos de Copaifera sabulicola sobre o desenvolvimento inicial de Lactuca sativa, Lycopersicum esculentum e Zea mays

“…Os efeitos alelopáticos decorrente dos extratos de C. sabulicola provavelmente são indícios da ocorrência de metabólitos secundários com função aleloquímica, concordando com os relatos na literatura da presença princípios ativos em algumas espécies deste gênero ( EIGA-J NIOR; PINTO, 2002;PEREIRA et al, 2008;DEUS et al, 2009;LEANDRO et al, 2012).…”