Detection of flavonoids in Alpinia purpurata (Vieill.) K. Schum. leaves using high-performance liquid chromatography

Abstract

ABSTRACT:The species Alpinia purpurata is scarcely cited as to ethnopharmacology and phytochemistry. This study aimed to analyze bioactive compounds through high-performance liquid chromatography (HPLC). Hydroalcoholic crude extract was obtained from A. purpurata dried leaves. Folin-Ciocalteau method was used to quantify total phenols, using gallic acid as standard. The obtained result was 15.6 mg GAE g -1 . The crude extract was partitioned with the solvents ethyl acetate and butanol, followed by thin-layer chromatography (TLC) and HPLC. The flavonoids kaempferol-3-O-glucuronide and rutin were detected at a higher concentration in ethyl acetate and butanolic extracts. The butanolic extract contains the highest flavonoid percentage (94.3%). A. purpurata presents important flavonoids of therapeutic use, already verified for A. zerumbet. This is the first study verifying the presence of flavonoids in A. purpurata extracts.Key words: TLC, polyphenols, medicinal plants, rutin, Zingiberaceae RESUMO: Detecção de flavonóides em folhas de Alpinia purpurata (Vieill.) K. Schum. por cromatografia líquida de alta eficiência. A espécie Alpinia purpurata apresenta poucas citações referentes a etnofarmacologia e fitoquímica. Este estudo propõe a análise de substâncias bioativas através da técnica de cromatografia líquida de alta eficiência (CLAE). O extrato bruto hidroalcóolico foi obtido a partir de folhas secas de A. purpurata. A quantificação de fenóis totais foi realizada pelo método de Folin-Ciocalteau, usando ácido gálico como padrão. Como resultado, foi verificado 15,6 mg EAG g -1 . O extrato bruto foi particionado com os solventes acetato de etila e butanol e depois analisado por cromatografia em camada delgada e CLAE. Nos extratos acetato de etila e butanólico foi detectada a presença dos flavonóides kaempferol-3-O-glicuronídeo e rutina, em maior concentração. O extrato butanólico contém a maior porcentagem de flavonóides (94,3%). Esta espécie possui flavonóides importantes no uso terapêutico, já antes verificados para a espécie A. zerumbet. Este é o primeiro trabalho que verifica a presença de flavonóides em extratos de A. purpurata.

Improvement of Bioactivity with Nanoparticle Fabrication: Cytotoxic Test of Ethanol, N-Hexane and Ethyl Acetate Extract from Red Galangal Rhizome (Alpinia purpurata (Vieill.) K. Schum) in Bulk and Nanoparticle size using BSLT Method

“…Pemanfaatan zat-zat kimia yang terkandung di dalam tumbuhan sangat penting, karena senyawa bahan alam yang aktif farmakologik yang digunakan sebagai obat umumnya efek sampingnya tidak ada atau ringan dibandingkan dengan obat dari senyawa sintetik. Selain itu sumber daya alam sebagai bahan awal mudah diperoleh dan diperbaharui [ [7], alkaloid [8], diarilheptanoid, steroids, kardioglikosida, minyak dan lemak, tannin, karbohidrat [9].…”

Metabolite Profiles of Arsenic Tolerant Plants Regenerated from Stem Calli of Andrographis paniculata (Burm.f.) Nees using HPLC and 1D 1H NMR

“…The samples were subsequently stored in separate sample bottles for further study. The modified method of Victório et al (2009) was applied for the extraction of plant material. The samples (2 gm each) were extracted with 20 ml methanol at room temperature for 24-h with occasional shaking.…”

Therapeutic value of the genus Alpinia, Zingiberaceae

“…Some flavonoids, such as cardarmomin and alpinetin (1), have already been identified in rhizomes and seeds of the species A. zerumbet and A. katsumadai (Krishna, 1973;Itokawa, 1981;He et al, 2005). Rutin, isoquercitrin, catechin, epicatechin, kaempferol (2), kaempferol-3- (Victório et al, 2007;Victório et al, 2009b;Victório et al 2009c;2009d;2010a). The flavonoid galangin was identified for the species A. officinarum (Tao et al, 2006).…”