2014
DOI: 10.1590/s1415-47572014005000016
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Abstract: Different types of water bodies, including lakes, streams, and coastal marine waters, are often susceptible to fecal contamination from a range of point and nonpoint sources, and have been evaluated using fecal indicator microorganisms. The most commonly used fecal indicator is Escherichia coli, but traditional cultivation methods do not allow discrimination of the source of pollution. The use of triplex PCR offers an approach that is fast and inexpensive, and here enabled the identification of phylogroups. Th… Show more

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Cited by 6 publications
(9 citation statements)
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“…All WTPs received domestic discharges, while Parque Novo Mundo and Barueri plants received also industrial discharges. Raw wastewater samples were collected in sterile bottles according to standard methods (APHA, 2010 ) and samples were analyzed using the membrane filter technique according to U.S. Environmental Protection Agency method 1603 (USEPA, 2002 ) as previously described by Stoppe et al ( 2014 ).…”
Section: Methodsmentioning
confidence: 99%
See 2 more Smart Citations
“…All WTPs received domestic discharges, while Parque Novo Mundo and Barueri plants received also industrial discharges. Raw wastewater samples were collected in sterile bottles according to standard methods (APHA, 2010 ) and samples were analyzed using the membrane filter technique according to U.S. Environmental Protection Agency method 1603 (USEPA, 2002 ) as previously described by Stoppe et al ( 2014 ).…”
Section: Methodsmentioning
confidence: 99%
“…Genomic DNA from E. coli strains was isolated with the Wizard Genomic DNA Purification Kit (Promega) according manufacturer's instructions. The phylogroup of E. coli strains was determined by triplex PCR as previously described by Clermont et al ( 2000 ) and Stoppe et al ( 2014 ).…”
Section: Methodsmentioning
confidence: 99%
See 1 more Smart Citation
“…The first alternative was the use of statistical methods -commonly applied for cluster identificationsuch as Correspondence Analysis (CA) and Unweight Par Group Method with Arithmetic mean (UPGMA). As described by [27], the CA did not recover any consistent grouping. The UPGMA clustering of the dissimilarities split the nodes into two groups, but these groups did not reflect features that are of interest for water studies, such as geographic location, source of pollution or abiotic factors.…”
mentioning
confidence: 70%
“…Phylogenetic subgroups (A 0 , A 1 , B1, B2 2 , B2 3 , D 1 and D 2 ) described by [11] and [12] were identified from Escherichia coli strains isolated from twelve water bodies with different pollution levels in the State of São Paulo as previously described by [27]. The phylogenetic subgroups were used as biomarkers in the rivers and reservoirs studied, which are Tietê River (TIET2050 and TIET3120), Paratei River (PTEI2900), Ipiranga River (IPIR0018), Pau de Bala Stream (PBAL0014), Aguapei River (AGUA2800), Jaguari Mirim River (JAMI2100), Tanque Grande Reservoir (TGDE0900), Billings Reservoir (BILL2801 and BILL2251) and Guarapiranga Reservoir (GUAR0502 and GUAR0601).…”
Section: Sample Descriptionmentioning
confidence: 99%