Validation of a microsatellite panel for parentage testing of locally adapted and commercial goats in Brazil

Silva, Elizabete Cristina da; McManus, Concepta; Pía, María; Lima, Souza; Guimarães, Mattos de Paiva; Pimentel, Concepta Margaret McManus; Caetano, Alexandre Rodrigues; Paiva, Samuel Rezende

doi:10.1590/s1415-47572014000100010

Cited by 9 publications

(9 citation statements)

References 39 publications

(53 reference statements)

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“…It is an important finding that the CPE values obtained from the paternity test panels formed by six or more microsatellites in the present study are in agreement with the values obtained from paternity test panels formed by eleven microsatellites reported by Luikart et al (24) and ISAG (26). At the same time, these values were similar to CPE values obtained from paternity test panels consisted of sixteen (18), twelve (27), seventeen (28), and eight (17) microsatellite markers. On the other hand, these values were higher than the values obtained from paternity test panels reported by the MAPA (29).…”

Section: Discussionsupporting

confidence: 81%

“…The mean number of alleles (MNa), mean expected heterozygosity (MHe), and mean polymorphic information content (MPIC) values obtained from the generated paternity test panels were higher than the values in Brazilian goat breeds reported by da Da Silva et al (18).…”

Section: Discussioncontrasting

confidence: 67%

“…Obtained MNa and PIC values were significantly higher than those of some other Turkish goat breeds and other goat breeds raised in different countries (17,18), while these values were lower than those of goat breeds raised in North Africa (19). The findings related to allele numbers indicated a high level of allelic richness in the breed studied.…”

Section: Discussionmentioning

confidence: 61%

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Microsatellite panels for parentage testing of Kilis goats reared in Turkey

Keskin¹,

Yılmaz²,

Gündüz³

et al. 2019

Turk J Vet Anim Sci

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The aim of this study was to develop PCR-based suitable microsatellite marker panels for paternity testing and to define pedigree errors in Kilis goats. A total of 137 head of goats were used, consisting of 118 head of kids and 19 head of possible candidate sires. A total of 392 alleles were observed in 22 microsatellite markers. Allele numbers ranged between 12 (SRCRSP7) and 24 (BM1818, INRA0023, and SRCRSP15) with an average of 17.82. The mean value of the effective allele numbers was obtained as 9.44. The overall polymorphic information content value was quite high (0.88). The overall observed (Ho) and expected heterozygosity (He) values for all studied loci were 0.89 and 0.89, respectively. Paternity test panels for the Kilis goat population studied were created based on individual probability of exclusion of microsatellites in multiplex groups. Combined probability of exclusion (CPE) values for different panels ranged between 0.745 (Panel-1) and 0.9999 , while the combined identification probability (CPI) values were obtained in the range of 9.81 × 10 -3 (Panel-1) to 6.96 × 10 -21 . As a result of this study, it can be stated that panels with 0.999 CPE values can be used at the most reasonable cost and with high reliability in paternity tests to be performed in Kilis goats, which can be a reference for other populations.

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Section: Discussionsupporting

confidence: 81%

Section: Discussioncontrasting

confidence: 67%

Section: Discussionmentioning

confidence: 61%

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Microsatellite panels for parentage testing of Kilis goats reared in Turkey

Keskin¹,

Yılmaz²,

Gündüz³

et al. 2019

Turk J Vet Anim Sci

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“…For more than a decade, microsatellites have been one of the most popular types of markers used in the livestock sector for various purposes [45], e.g., the characterization and conservation of diversity [46,47], the reconstruction of the post-domestication evolutionary history of farm animals [48,49], parentage testing [50], mapping of quantitative trait loci (QTL) [51,52] or other causative mutations [53], and traceability of food products [26,54,55]. The average number of microsatellite loci used in livestock research varied between 15 and 30 [45], even if a lower number of highly informative loci have been adopted for specific purposes.…”

Section: Microsatellite Markers In the Livestock Sectormentioning

confidence: 99%

Use of Microsatellites to Study Agricultural Biodiversity and Food Traceability

Bernardi¹,

Colli²,

Ughini³

et al. 2016

Microsatellite Markers

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Molecular markers are useful tools for measuring the genetic diversity among agricultural species. In plants, microsatellites are still the most used markers for germplasm characterization, conservation, and traceability purposes, while in the livestock sector, although having represented the standard for at least two decades, they are still used only for minor farm animal species. In this work, together with a review on the use of microsatellites in livestock, we also illustrate the use of these markers for the characterization of agricultural diversity and food traceability through two case studies: (i) the analysis of genetic diversity in ancient fruit tree cultivars of apple (Malus × domestica Borkh.), pear (Pyrus communis L.), sweet cherry (Prunus avium L.), and sour cherry (Prunus cerasus L.) from Northern Italy and (ii) the molecular authentication of wheat food chain. In the former case, a high genetic variability as well as the presence of different ploidy levels were detected, while in the latter microsatellite markers were shown to be useful for traceability and product authentication along the whole food chain. Overall, the presented evidence confirms the versatility of microsatellites as markers for both agrobiodiversity characterization and food traceability in cultivated plants and farm animals.

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“…It is concluded that the protocol used for extracting was efficient of DNA for the species in question, necessitating further of new studies with markers microsatellite. KEYWORDS: Capra hircus, genetic diversity, molecular marker, genetic variability INTRODUÇÃO A caprinocultura é uma atividade de grande importância econômica e social no Brasil (SILVESTRE et al, 2015) e no mundo, devido a produção de leite e de corte, tornando os caprinos um dos principais animais de produção (SILVA et al, 2014a), havendo um crescente aumento quanto ao número de rebanhos e produtividade dos mesmos .…”

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Marcadores Microssatélites Para Caracterização Genética De Caprinos Da Raça Saanen Na Região Sul Do Estado Do Espírito Santo - Brasil

Vieira¹,

Alfaiate²,

Oliveira³

et al. 2015

Enciclopédia Biosfera

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RESUMOObjetivou-se utilizar marcadores microssatélites para caracterizar geneticamente os caprinos da raça Saanen da região sul do estado do Espírito Santo. O protocolo de extração de DNA utilizado nos bulbos pilosos foi o protocolo de lise, onde os materiais resultantes deste foram submetidos à amplificação via PCR. Foram testados trinta e um iniciadores com diferentes gradientes de temperatura. Os produtos finais da PCR foram submetidos à eletroforese, revelados e visualizados com auxílio de fotodocumentador. O protocolo de lise utilizado para extração do DNA foi eficiente. Contudo não houve amplificação de nenhum marcador microssatélite, indicando a não eficiência dos diferentes gradientes de temperatura testados, bem como, quanto aos iniciadores utilizados, sendo necessária nova triagem. Conclui-se que o protocolo para extração foi eficiente para a espécie em questão, necessitando-se ainda, de uma nova triagem dos iniciadores microssatélites. ABSTRACTThe objective of this study was to use microsatellite markers to characterize genetically the goats of breed Saanen in the south region of state Espírito Santo -Brazil. The DNA extraction protocol used in hair bulbs was the lysis protocol, where the materials resulting from this were submitted to amplification by PCR. Thirty-one primers with different temperature gradients were tested. The end products of the

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Validation of a microsatellite panel for parentage testing of locally adapted and commercial goats in Brazil

Cited by 9 publications

References 39 publications

Microsatellite panels for parentage testing of Kilis goats reared in Turkey

Microsatellite panels for parentage testing of Kilis goats reared in Turkey

Use of Microsatellites to Study Agricultural Biodiversity and Food Traceability

Marcadores Microssatélites Para Caracterização Genética De Caprinos Da Raça Saanen Na Região Sul Do Estado Do Espírito Santo - Brasil

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