Molecular characterization of MHC-DRB cDNA in water buffalo (Bubalus bubalis)

Naskar, S.; Deb, S. M.; Niranjan, S. K.; Kumar, Subodh; Sharma, Deepak; Sakaram, D.; Sharma, Arjava

doi:10.1590/s1415-47572012005000013

Cited by 3 publications

(2 citation statements)

References 14 publications

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“…in cattle ( Sharma et al, 2006; Huang et al, 2011 ). Maximum homology (99%) of 5′upstream region of buffalo TLR sequence with that of goat was observed to be in concordance with previous reports on TLR8 ( Dubey et al, 2012a,b ) and DRB * A ( Naskar et al, 2012 ) genes, wherein the water buffalo was observed to be phylogenetically more closer to goat than cattle. High homology of TLR8 region between riverine buffalo and goat suggests that TLR genes have evolved due to challenging by different types of species specific pathogens within populations of livestock species.…”

Section: Discussionsupporting

confidence: 90%

Genetic diversity within 5′upstream region of Toll-like receptor 8 gene reveals differentiation of riverine and swamp buffaloes

et al. 2013

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In this study the nucleotide diversity in the 5′untranslated region (UTR) of TLR8 gene in riverine as well as swamp buffaloes has been described. Analysis of the 5′UTR of TLR8 gene showed presence of two SNPs in this region, g.-139G>T and g.-128A>G. A PCR–RFLP assay designed for genotyping of g.-139G>T SNP across 667 samples from 2 buffalo populations revealed a striking difference in allele frequency distribution across the swamp and riverine buffaloes. The frequency of T allele was higher in swamp buffalo as compared to riverine buffalo, ranging from 0.71 to 1. The G allele on the other hand exhibited a higher frequency across all the Indian riverine buffalo breeds/populations. The principal component analysis revealed separate clusters for the riverine and swamp buffaloes, as expected; however, the riverine type Assamese buffalo population of eastern India formed a distinct cluster. Since most of the buffalo populations in the eastern region are swamp type, this demarcation may be related to the difference in immune response in riverine and swamp buffaloes. These preliminary results indicate that the genetic variation observed in 5′upstream region of TLR8 gene, which differentiates swamp and riverine buffalo, needs to be further explored for association with disease susceptibility in buffalo, an important dairy and meat animal of Southeast Asia.

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Section: Discussionsupporting

confidence: 90%

Genetic diversity within 5′upstream region of Toll-like receptor 8 gene reveals differentiation of riverine and swamp buffaloes

et al. 2013

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“…Under the DR region of the MHC complex, highly polymorphic β chain encoded by DRB genes is found (Zhao et al, 2011). DRA gene encodes α chain whereas β chain is coded by DRB gene (Naskar et al, 2012). Three BoLA (bovine leukocyte antigen) DRB genes (DRB1, 2 and 3) are reported in bovines but only DRB3 is functional (Singh et al, 2012).…”

Section: Structure and Function Of Drb3 Genementioning

confidence: 99%

Multifunctional DRB3, a MHC Class II Gene, as a Useful Biomarker in Small Ruminants: A Review

Paracha¹

2015

J. Inf. Mol. Biol.

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Major histocompatibility complex (MHC) is a large family of genes present in various vertebrates and is well known for the diversity of its alleles. These genes play an important role in the recognition of foreign antigens and mediating an immune response. MHC is classified as Class I, II and III molecules based on their molecular weights as well as differences in their cellular distribution and function. DRB3 gene is a member of MHC Class II and is known to show extensive polymorphism. This gene is being used as marker in phylogenetic and molecular genetics. This paper is aimed to review the role of DRB3 gene in goats.

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Association of SNP variants of MHC Class II DRB gene with thermo-physiological traits in tropical goats

Yakubu

Salako

Donato

et al. 2016

Trop Anim Health Prod

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Host defense in vertebrates depend on many secreted regulatory proteins such as major histocompatibility complex (MHC) class II which provide important regulatory and effector functions of T cells. Gene polymorphism in the second exon of Capra-DRB gene in three major Nigerian goat breeds [West African Dwarf (WAD), Red Sokoto (RS), and Sahel (SH)] was analyzed by restriction fragment length polymorphisms (RFLP). Four restriction enzymes, BsaHI, AluI, HaeIII, and SacII, were utilized. The association between the polymorphic sites and some heat tolerance traits were also investigated in a total of 70 WAD, 90 RS, and 50 SH goats. Fourteen different types of alleles identified in the Nigerian goats, four of which were found in the peptide coding region (A57G, Q89R, G104D, and T112I), indicate a high degree of polymorphism at the DRB locus in this species. An obvious excess (P < 0.01) of non-synonymous substitutions than synonymous (dN/dS) in this locus is a reflection of adaptive evolution and positive selection. The phylogenetic trees revealed largely species-wise clustering in DRB gene. BsaHI, AluI, HaeIII, and SacII genotype frequencies were in Hardy-Weinberg equilibrium (P > 0.05), except AluI in RS goats and HaeIII in WAD goats (P < 0.05). The expected heterozygosity (H), which is a measure of gene diversity in the goat populations, ranged from 0.16 to 0.50. Genotypes AA (BsaHI), GG, GC and CC (AluI) and GG, GA, AA (HaeIII) appeared better in terms of heat tolerance. The heat-tolerant ability of SH and RS goats to the hot and humid tropical environment of Nigeria seemed better than that of the WAD goats. Sex effect (P < 0.05) was mainly on pulse rate and heat stress index, while there were varying interaction effects on heat tolerance. Variation at the DRB locus may prove to be important in possible selection and breeding for genetic resistance to heat stress in the tropics.

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Molecular characterization of MHC-DRB cDNA in water buffalo (Bubalus bubalis)

Cited by 3 publications

References 14 publications

Genetic diversity within 5′upstream region of Toll-like receptor 8 gene reveals differentiation of riverine and swamp buffaloes

Genetic diversity within 5′upstream region of Toll-like receptor 8 gene reveals differentiation of riverine and swamp buffaloes

Multifunctional DRB3, a MHC Class II Gene, as a Useful Biomarker in Small Ruminants: A Review

Association of SNP variants of MHC Class II DRB gene with thermo-physiological traits in tropical goats

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