“…Currently, the major researches on soybean proteomics included expression differences of proteins in leaves, roots, seeds and seed linage [13,14,15,16], the expression differences, in root, leaves, flower buds, seeds under different growth and stress conditions [17,18,19,20,21], the researches on low abundant proteins [22,23,24], the allergen protein studies [25,26,27], the analysis on proteins of transgenosis and normal soybeans [28], the quantitative analysis on proteomic profiles [29,30]. In all these works, the mainly used techniques are some separation technologies, such as 2-DE [1,13,15,19~23,25,28,30,31,32,33], SDS-PAGE [14,24,29,31], HPLC-MS [1,14,20,24], by which the overall expression spectra of proteome could be obtained.…”