2011
DOI: 10.1590/s1415-47572011005000059
|View full text |Cite
|
Sign up to set email alerts
|

Abstract: Traditional phenotypic methods and commercial kits based on carbohydrate assimilation patterns are unable to consistently distinguish among isolates of Pichia guilliermondii, Debaryomyces hansenii and Candida palmioleophila. As result, these species are often misidentified. In this work, we established a reliable method for the identification/differentiation of these species. Our assay was validated by DNA sequencing of the polymorphic region used in a real-time PCR assay driven by species-specific probes targ… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
4

Citation Types

2
4
0

Year Published

2014
2014
2019
2019

Publication Types

Select...
5
1

Relationship

0
6

Authors

Journals

citations
Cited by 11 publications
(6 citation statements)
references
References 15 publications
2
4
0
Order By: Relevance
“…With regards to antifungal resistance, 3% of the isolates were resistant to amphotericin; which was consistent with the results of another study (6.9%). This resistance was due to prior extensive use of amphotericin B in immunocompromised patients, unlike in other studies that reported no amphotericin B resistance 2325. The present study showed that 27% of the isolates, mostly C. albicans and not C. tropicalis were resistant to itraconazole resistance; which agreed ith some of the previous studies 24,25.…”
Section: Discussionsupporting
confidence: 89%
See 1 more Smart Citation
“…With regards to antifungal resistance, 3% of the isolates were resistant to amphotericin; which was consistent with the results of another study (6.9%). This resistance was due to prior extensive use of amphotericin B in immunocompromised patients, unlike in other studies that reported no amphotericin B resistance 2325. The present study showed that 27% of the isolates, mostly C. albicans and not C. tropicalis were resistant to itraconazole resistance; which agreed ith some of the previous studies 24,25.…”
Section: Discussionsupporting
confidence: 89%
“…This resistance was due to prior extensive use of amphotericin B in immunocompromised patients, unlike in other studies that reported no amphotericin B resistance 2325. The present study showed that 27% of the isolates, mostly C. albicans and not C. tropicalis were resistant to itraconazole resistance; which agreed ith some of the previous studies 24,25. Caspofungin resistance rates remained low; the present study found that 1% of the isolates were resistant to caspofungin 26…”
Section: Discussionsupporting
confidence: 88%
“…These species were found in the present study as well. It is also difficult to phenotypically identify cryptic species within species complexes without the addition of molecular sequencing 8,20,34 methods. This was observed with the isolates of the Parapsilosis complex and those of C. dubliniensis and C. lusitaniae (Fig.…”
Section: Discussionmentioning
confidence: 99%
“…In [ 51 ], Desnos-Ollivier et al analyzed the dozens of isolates of different origin with above synonyms and their aim was to assess, using nucleotide sequences, whether phenotypic identification was correct. They concluded, like others [ 52 ], that sequencing of ITS (or D1/D2 region) is a good tool for differentiating the species more frequently confused with D. hansenii.…”
Section: Discussionmentioning
confidence: 56%