A polymorphic microsatellite from the Squalius alburnoides complex (Osteichthyes, Cyprinidae) cloned by serendipity can be useful in genetic analysis of polyploids

Boto, Luis; Cunha, Carolina; Doadrio, Ignacio

doi:10.1590/s1415-47572011005000023

Cited by 2 publications

(2 citation statements)

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“…The A genome that is present in all S. alburnoides has distinctly different microsatellite sizes from those found in P or C genomes [Boto et al, 2011;Cunha et al, 2011], which suggests that recombinations do not occur between homeologous chromosomes. However, Rampin et al [2012], using GISH, documented a possible incident of genome exchange between P and A chromosomes.…”

Section: Polyploid European Fish In the Squalius Alburnoides Complexmentioning

confidence: 96%

Genetic and Genomic Interactions of Animals with Different Ploidy Levels

Bogart¹,

Bi²

2013

Cytogenet Genome Res

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Polyploid animals have independently evolved from diploids in diverse taxa across the tree of life. We review a few polyploid animal species or biotypes where recently developed molecular and cytogenetic methods have significantly improved our understanding of their genetics, reproduction and evolution. Mitochondrial sequences that target the maternal ancestor of a polyploid show that polyploids may have single (e.g. unisexual salamanders in the genus Ambystoma) or multiple (e.g. parthenogenetic polyploid lizards in the genus Aspidoscelis) origins. Microsatellites are nuclear markers that can be used to analyze genetic recombinations, reproductive modes (e.g. Ambystoma) and recombination events (e.g. polyploid frogs such as Pelophylax esculentus). Hom(e)ologous chromosomes and rare intergenomic exchanges in allopolyploids have been distinguished by applying genome-specific fluorescent probes to chromosome spreads. Polyploids arise, and are maintained, through perturbations of the ‘normal' meiotic program that would include pre-meiotic chromosome replication and genomic integrity of homologs. When possible, asexual, unisexual and bisexual polyploid species or biotypes interact with diploid relatives, and genes are passed from diploid to polyploid gene pools, which increase genetic diversity and ultimately evolutionary flexibility in the polyploid. When diploid relatives do not exist, polyploids can interact with another polyploid (e.g. species of African Clawed Frogs in the genus Xenopus). Some polyploid fish (e.g. salmonids) and frogs (Xenopus) represent independent lineages whose ancestors experienced whole genome duplication events. Some tetraploid frogs (P. esculentus) and fish (Squaliusalburnoides) may be in the process of becoming independent species, but diploid and triploid forms of these ‘species' continue to genetically interact with the comparatively few tetraploid populations. Genetic and genomic interaction between polyploids and diploids is a complex and dynamic process that likely plays a crucial role for the evolution and persistence of polyploid animals. See also other articles in this themed issue.

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Section: Polyploid European Fish In the Squalius Alburnoides Complexmentioning

confidence: 96%

Genetic and Genomic Interactions of Animals with Different Ploidy Levels

Bogart¹,

Bi²

2013

Cytogenet Genome Res

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“…Microsatellites markers have been developed for several Cyprinidae species, including Squalius aradensis, S. cephalus, Leuciscus souffia, L. idus, and L. leuciscus (Mesquita et al 2003;Barinova et al 2004;Larno et al 2005;Vyskocilova et al 2007;Muenzel et al 2008;Dubut et al 2009;Boto et al 2011). Furthermore previous studies pointed out the usefulness of cross-species amplification of microsatellites in Cyprinidae to establish markers for population genetics studies (Muenzel et al 2008;Dubut et al 2010).…”

Section: Discussionmentioning

confidence: 99%

Isolation of microsatellite markers inSqualius lucumonis(Bianco, 1983) and cross-species amplification within the family Cyprinidae and other freshwater fish species

Gigliarelli¹,

Puletti²,

Giannetto³

et al. 2012

Italian Journal of Zoology

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The Etruscan chub Squalius lucumonis (Bianco, 1983) is an endemic endangered fish inhabiting the lakes and rivers of Central Italy. A microsatellite-enriched genomic library was constructed in order to develop microsatellite loci and eight polymorphic loci were isolated from this species to assess the level of genetic diversity in fifty individuals of S. lucumonis collected in the Umbria Region. The number of alleles ranged from four (Sluc12) to 18 (Sluc4). Observed and expected heterozygosity per locus ranged from 0.05 (Sluc12) to 0.80 (Sluc4; Sluc7 and Sluc11) and from 0.22 (Sluc12) to 0.90 (Sluc4), respectively. Furthermore, these loci were tested on ten other Cyprinidae species and four freshwater fish species belonging to other families. Successful cross-priming amplifications were obtained for several species of Cyprinidae, whereas no positive results were obtained for other species. The availability of the reported microsatellite loci will facilitate population structure investigations of these species aiming to phylogeographical approaches and conservation strategies.

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A polymorphic microsatellite from the Squalius alburnoides complex (Osteichthyes, Cyprinidae) cloned by serendipity can be useful in genetic analysis of polyploids

Cited by 2 publications

References 40 publications

Genetic and Genomic Interactions of Animals with Different Ploidy Levels

Genetic and Genomic Interactions of Animals with Different Ploidy Levels

Isolation of microsatellite markers inSqualius lucumonis(Bianco, 1983) and cross-species amplification within the family Cyprinidae and other freshwater fish species

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